Thermo Scientific™

Tango™ CCR1-bla U2OS DA Assay Kit

Catalog number:  K1793

Related applications:

Pharma & Biopharma

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The Tango™ CCR1-bla U2OS DA cells contain the human Chemokine (C-C Motif) Receptor 1 (CCR1) linked to a TEV protease site and a Gal4-VP16 transcription factor stably integrated into the Tango™ GPCR-bla U2OS parental cell line. This parental cell line stably expresses a beta-arrestin⁄TEV protease fusion protein and the beta-lactamase (bla) reporter gene under the control of a UAS response element.

The Tango™ CCR1-bla U2OS DA cells have been functionally validated for Z' factor and EC50 concentrations of established ligands. In addition, Tango™ CCR1-bla U2OS DA cells have been tested for assay performance under variable conditions. These data are found in the Validation & Assay Performance Summary for each product. Division Arrested cells are irreversibly division arrested using a low-dose treatment of Mitomycin C, and have no apparent toxicity or change in cellular signal transduction.
For Research Use Only. Not for use in diagnostic procedures.


Accession Number: NM_001295
Assay Entry: Cell-based beta-arrestin recruitment
Cell Line: U2OS
Cell State: Division-Arrested Cells
Cytoplasmic Tail: Tail from GRPR GPCR
Detection Method: Fluorescent
Druggable Target: GPCRs
Gene Symbol: CCR1
Population Status: Single Cell Clone Origin
Product Line: GeneBLAzer®
Protein Family: GPCR (G-Protein Coupled Receptors)
Readout: End Point
Reporter Gene: BLA (Beta-Lactamase)
System Type: Tango™
Target Entry: CCR1

Contents & storage

K1793 Tango™ CCR1-bla U2OS DA Assay Kit
Each kit contains sufficient quantities of division arrested (DA) cells and substrate to assay 1 x 384 well plate. (Other materials are required separately; please refer to the protocol).
• Tango™ CCR1-bla U2OS DA cells
• LiveBLAzer™-FRET B⁄G Loading Kit, 70µg
• Solution D
• Tango™ CCR1-bla U2OS DA Assay Protocol
• LiveBLAzer™-FRET B⁄G Loading Protocol
• Certificate of Analysis
The Tango™ CCR1-bla U2OS DA cells are shipped on dry ice and should be stored in liquid nitrogen immediately upon receipt.