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PA1-40301 detects E-Selectin from human, chimpanzee, rhesus monkey, cynomolgous monkey and baboon samples.
PA1-40301 reacts with the Lectin Domain of E-selectin, CD62-e, formerly designated Endothelial Leucocyte Adhesion Molecule-1 (ELAM-1). PA1-40301 reacts with human endothelial cells activated with TNF, IL-1 or endotoxin. PA1-40301 was found to react also with cells transfected with the ELAM-1 gene. The antibody inhibits the adhesion of granulocytes both neutrophilic and eosinophilic.
PA1-40301 has been successfully used in ELISA, immunohistochemistry (frozen) and Western blot applications. It is recommended that users test the reagent and determin their own optimal dilutions: the typical starting working dilution is 1:10.
The PA1-40301 imunogen is human E-Selectin.
Leukocytes adhere to the blood vessel endothelium during extravasation in postcapillary venules of lymph nodes. In addition, leukocyte adhesion occurs in the capillaries and small venules at any site in the body after onset of inflammation. This response is immediate and involves a cascade of adhesion receptors. At the endothelial surface members of the selectin and immunoglobulin superfamilies participate in this cascade. Selectins are C-type cell surface lectins that play a role in leukocyte adhesion to the blood vessel wall endothelium. E-selectin (CD62E) is an endothelial cell specific selectin that is expressed only after activation with proinflammatory cytokines. In vitro experiments have shown that IL-1, TNFalpha and bacterial wall components like lipopolysaccharides induce the transcription of E-selectin in a NFkB dependent signaling cascade. E-selectin has been associated with blood vessel endothelium in diverse inflammatory situations.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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