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The Mouse Luteinizing Hormone (LH) ELISA quantitates LH in serum, plasma, and other biological fluids
The Mouse Luteinizing Hormone (LH) solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the binding of the second (detector) antibody to the target on a different epitope from the capture antibody. An antibody conjugated with enzyme binds the formed sandwich. After incubation and washing steps to rid the microplate of unbound substances, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation:
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
LH is a member of the glycoprotein hormone family, Glycoprotein hormones are heterodimers consisting of a common alpha subunit and an unique beta subunit which confers biological specificity. LH is expressed in the pituitary gland and promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids. The genes for the beta chains of chorionic gonadotropin and for luteinizing hormone are contiguous on chromosome 19q13.3. Mutations in this gene are associated with hypogonadism which is characterized by infertility and pseudohermaphroditism.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Gene aliases : aGSU, alpha-GSU, alphaGSU, alphaSU, CG-alpha, Cga, FSHA, GPHA1, GPHalpha, HCG, LH, LH-B, LHA, LSH-B, LSH-beta, Tsha
Gene ID : (Mouse) 12640, (Mouse) 16866
Gene symbol : Cga, Lhb
Protein Aliases : Anterior pituitary glycoprotein hormones common subunit alpha, Follicle-stimulating hormone alpha chain, Follitropin alpha chain, FSH-alpha, Glycoprotein hormones alpha chain, leutropin, LSH-alpha, Luteinizing hormone alpha chain, luteinizing hormone subunit beta, Lutropin alpha chain, lutropin beta chain, lutropin subunit beta, Thyroid-stimulating hormone alpha chain, Thyrotropin alpha chain, TSH-alpha
UniProt ID (Mouse) P01216
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