Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
The Human Fibroblast Growth Factor 1 (Hu FGF1/FGFα) ELISA quantitates Hu FGF1 in human serum, plasma, or cell culture medium. The assay will exclusively recognize both natural and recombinant Hu FGF1.
Principle of the method
The Human FGF1 solid-phase sandwich ELISA (enzyme-linked immunosorbent assay) is designed to measure the amount of the target bound between a matched antibody pair. A target-specific antibody has been pre-coated in the wells of the supplied microplate. Samples, standards, or controls are then added into these wells and bind to the immobilized (capture) antibody. The sandwich is formed by the addition of the second (detector) antibody, a substrate solution is added that reacts with the enzyme-antibody-target complex to produce measurable signal. The intensity of this signal is directly proportional to the concentration of target present in the original specimen.
Rigorous validation
Each manufactured lot of this ELISA kit is quality tested for criteria such as sensitivity, specificity, precision, and lot-to-lot consistency. See manual for more information on validation.
FGF1 is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Gene aliases : AFGF, ECGF, ECGF-beta, ECGFA, ECGFB, FGF-1, FGF-alpha, FGF1, FGFA, GLIO703, HBGF-1, HBGF1
Gene ID : (Human) 2246
Gene symbol : FGF1
Protein Aliases : Acidic fibroblast growth factor, aFGF, beta-endothelial cell growth factor, ECGF, Endothelial cell growth factor, endothelial cell growth factor, alpha, beta, FGF-1, Fibroblast growth factor 1, fibroblast growth factor 1 (acidic), HBGF-1, Heparin-binding growth factor 1
UniProt ID (Human) P05230
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