Shop All Cell Culture Supplements

Glucose Solution Gibco™

Gibco® Glucose Solution contains D-glucose at a concentration of 200 g/L and is used as a serum-free medium supplement suitable for cell culture. Glucose Solution can be added to a variety of Gibco® cell culture media including but not limited to Dulbecco’s Modified Eagle Medium (DMEM), RPMI 1640, DMEM/F-12, IMDM, and Minimum Essential Medium. Each lot of Glucose Solution is tested for sterility, osmolality, pH, and endotoxin level. We offer a variety of media to which Glucose Solution can be added. Find the right medium using the media selector tool.

cGMP Manufacturing and Quality System
Glucose Solution is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

Resurge™ CD3 Supplement, without glucose and L-glutamine Gibco™

Resurge CD3 Supplement, without glucose and L-glutamine, is an animal origin-free (AOF) protein-free chemically defined supplement. It can be used in batch and fed batch mode in cell culture processes. All components are chemically defined with known molecular formulae and structures and meet our stringent Animal Origin-Free (AOF) specification.

Resurge CD3 Supplement is formulated without glucose, L-glutamine, and sodium bicarbonate. Due to the balanced nutritional nature of this supplement, rich in amino acids, it has also been used in bacterial applications.

Features:
• Animal origin-free
• Chemically defined
• Protein-free
• Rich in amino acids

Applications of the Resurge CD3 Supplement, without glucose and L-glutamine, include:
• Can be used in batch and fed batch mode in cell culture processes
• Use in bacterial applications

Bovine Pituitary Extract (BPE) Gibco™

The protein concentration of each lot of Bovine Pituitary Extract is determined by the Bradford method. Each vial of BPE contains not less than 25 mg of protein.

Transferrin, Bovine (APO form), lyophilized Gibco™

Iron <50 µg/g protein.

Suggested range for supplementation: 0.5 to 50 µg/ml.

Origin:
New Zealand

L-Glutamine (200 mM)

Gibco® L-glutamine is an amino acid required for cell culture. L-glutamine participates in the formation of purine and pyrimidine nucleotides, amino sugars, glutathione, L-glutamate and other amino acids, as well as in protein synthesis and glucose production. L-Glutamine, unlike most other amino acids, is not stable in solution. The rate at which this degradation proceeds is a function of time, temperature and pH. We also offer Gibco® GlutaMAX™ supplement as a stable alternative to L-glutamine.

Gibco® L-glutamine 200 mM Solution is a stock solution ready-to-use as a cell culture supplement. The optimal use concentration is dependent upon the cell type and medium used to culture the cells, but generally falls in the range of 2 – 6 mM.

Dual-site cGMP Manufacturing and Quality System
Gibco® L-glutamine is manufactured at a cGMP compliant facility, located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical Gibco® L-glutamine product made in our Grand Island facility (25030-164). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.

Autolyzed Yeast Extract (15% solution) Gibco™

Gibco® Autolyzed Yeast Extract, 15% Solution is a cell culture supplement formulated as 150 g Yeast Extract Powder (YEP) per liter of distilled water. YEP is a concentrated extract of soluble amino acids, carbohydrates, vitamins and minerals produced from ultra-filtered Baker's yeast.

Product Use
For Research Use Only. Not for use in diagnostic procedures.

cGMP Manufacturing and Quality System
Gibco® Autolyzed Yeast Extract 15% Solution is manufactured at a cGMP compliant facility, located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.

FunctionMAX™ TiterEnhancer, AGT™ additive Gibco™

FunctionMAX™ TiterEnhancer uses a new technology to combine key nutrients in a highly concentrated (150 g/L), pH-neutral, chemically-defined, AOF (animal origin-free) functional additive that is designed to reinforce existing feeds and amplify the productivity of standard high cell density fed-batch platforms. FunctionMAX™ TiterEnhancer is available in both a liquid format and this AGT™ dry format and is stable for over 1 year. FunctionMAX™ TiterEnhancer offers:

• Up to double prior fed-batch titers
• Highly concentrated ingredients
• Easy to use pH-neutral format
• Specialized delivery of ingredients found in base media—nothing exotic
• Use that is economical

When to Choose FunctionMAX™ TiterEnhancer:

• If you have a drop off in specific productivity late in a high density fed-batch culture, FunctionMAX™ TiterEnhancer may be able to improve your titers
• If you have an extremely low peak cell density fed-batch culture, you may want to consult with PD-Direct® Services for advice on cell adaptation and/or fed-batch optimization
• If you have no drop off in specific productivity late in a high density fed-batch culture and still need higher titers, you may want to consult with PD-Direct® Services for advice on fed-batch optimization

Up to Double Prior Fed-batch Titers
We have demonstrated 35–120% product titer improvements when FunctionMAX™ TiterEnhancer was added on top of existing processes or as a partial feed replacement (Figures 1–3), resulting in a lower total proportion of feed addition. These increases were achieved primarily by maintaining specific productivity throughout each fed-batch process. Improvements were seen immediately and without process optimization. We have also shown that low-volume addition of FunctionMAX™ TiterEnhancer was sufficient to improve titers for several processes using different commercially available media and feeds, with those having higher cell densities seeing the most benefit. This additive is not designed to completely replace other feeding supplements that provide additional ingredients not found in FunctionMAX™ TiterEnhancer.

Highly Concentrated Ingredients
The technology used to deliver this functional additive promises to transform recombinant drug bioprocessing by raising key ingredient concentrations to levels that are out of reach using conventional approaches. A concentration of 150 g/L is achieved with FunctionMAX™ TiterEnhancer (compared to 20–25 g/L for most basal media), and it has been shown to be stable in dry or liquid format for over 1 year.

Easy-to-Use pH-neutral Format
Reconstitution of the AGT™ dry format is surprisingly simple (add water and stir), with all components going into solution. There is no pH adjustment required, and being pH neutral it is safer in handling and use than extreme pH alternatives. As a pH neutral additive, this product will help avoid bioreactor complications often seen with extreme pH supplements. Further, the liquid is very stable—so much so that the liquid format version is made from the same AGT™ dry format material that is also available for purchase. The liquid is provided for small-scale trials and the AGT™ dry format for seamless scale-up in use.

Specialized Delivery of Ingredients Found in Base Media—Nothing Exotic
FunctionMAX™ TiterEnhancer does not introduce new ingredients to your fed-batch process; it simply provides the same key ingredients found in basal media that the cells need late in culture to maintain their productivity. Further, the technology used to combine these ingredients does not introduce any additional ingredients. Nor does it contain any growth factors or undefined components like hydrolysates. A DMF (drug master file) reference can be requested for this product for assurance of regulatory authorities that there are no substances or ingredients in the formulation that might create regulatory concerns.

Economies in Use
FunctionMAX™ TiterEnhancer is designed to deliver highly concentrated and high value addition to your fed-batch process. Consider that it provides 7-fold the ingredient concentration of a basal medium and can provide up to a doubling of titer. Further, in normal use at about 10% of working volume, the actual price per liter of use is similar to that of basal media.

Large Vessel Endothelial Supplement (LVES) Gibco™

Gibco® Large Vessel Endothelial Supplement (LVES) is a 50X, complete, ready-to-use media supplement optimized for use with Gibco® Medium 200 and Medium 200PRF for culture of primary human umbilical vein endothelial cells (HUVEC), human aortic endothelial cells (HAEC), and human pulmonary artery endothelial cells (HPAEC). This blended supplement has been designed to deliver optimal cell growth and to enable superior cell performance for angiogenesis applications, such as cell migration and tube formation. LVES contains fetal bovine serum, hydrocortisone, human epidermal growth factor, basic fibroblast growth factor, heparin, and ascorbic acid.

• Perform tube formation, cell invasion, cell migration, and cell proliferation functional assays
• Achieve superior growth rates of endothelial cells compared to low serum growth supplement (LSGS)
• Expand primary large vessel endothelial cells without genomic alteration

Verified Performance in Key Angiogenesis Applications
Large Vessel Endothelial Supplement is designed to deliver optimal performance in tube formation, cell invasion, cell migration, and cell proliferation assays when used in conjunction with Medium 200, human umbilical vein endothelial cells, and Geltrex® LDEV-Free Reduced Growth Factor Basement Membrane Matrix. These items and a detailed protocol are available in a convenient Angiogenesis Starter Kit or they can be purchased separately.

Maximum Primary Cell Growth with One Blended Supplement
Large Vessel Endothelial Supplement is a complete, ready-to-use blend of growth supplements optimized to deliver>4 x 104 HUVEC per cm2 when used with Medium 200 or Medium 200PRF. Cell phenotype is retained and verified positive for von Willebrand factor (vWf) and CD31, negative for a-actin, and positive for dil-Ac-LDL uptake via ICC.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

Resurge™ CD1 Supplement, without glucose and L-glutamine Gibco™

Resurge CD1 Supplement, without glucose and L-glutamine, is a chemically defined supplement formulation to complement a variety of base media. This supplement was developed using several host cell lines and in various culture systems, including shaker flasks and bench-scale bioreactors. Resurge CD1 Supplement, without glucose and L-glutamine, was developed for use in CHO mammalian culture systems and can be used either as a media supplement or a culture system feed.

B-27™ Supplement (50X), minus vitamin A Gibco™

A variation of the most cited neuronal cell culture supplement, B-27 Supplement Minus Vitamin A is a customized version of the original B-27 Supplement without vitamin A (retinyl acetate). B-27 Supplement Minus Vitamin A is a serum-free supplement ideal for the cultivation of neural progenitor and stem cells, either as neurospheres in suspension or in adherent monolayer culture, without inducing differentiation. B-27 Supplement Minus Vitamin A is provided as a 50X liquid and is intended for use with Neurobasal Medium or Neurobasal-A Medium for neuronal cell culture without the need for an astrocyte feeder layer.

B-27 Supplement is suitable for use in most neuronal cell applications. For other applications, we provide alternative formulations of B-27 Supplement and Neurobasal media.

For established protocols using B-27 Supplement, access our Gibco Neurobiology Protocol Handbook.

View publications using Gibco neurobiology products at our Gibco CellCite.

FunctionMAX™ TiterEnhancer Gibco™

FunctionMAX™ TiterEnhancer uses a new technology to combine key nutrients in a highly concentrated (150 g/L), pH-neutral, chemically-defined, AOF (animal origin-free) functional additive that is designed to reinforce existing feeds and amplify the productivity of standard high cell density fed-batch platforms. FunctionMAX™ TiterEnhancer is available in both this liquid format and an AGT™ dry format and is stable for over 1 year. FunctionMAX™ TiterEnhancer offers:

• Up to double prior fed-batch titers
• Highly concentrated ingredients
• Easy to use pH-neutral format
• Specialized delivery of ingredients found in base media—nothing exotic
• Use that is economical

When to Choose FunctionMAX™ TiterEnhancer:

• If you have a drop off in specific productivity late in a high density fed-batch culture, FunctionMAX™ TiterEnhancer may be able to improve your titers
• If you have an extremely low peak cell density fed-batch culture, you may want to consult with PD-Direct® Services for advice on cell adaptation and/or fed-batch optimization
• If you have no drop off in specific productivity late in a high density fed-batch culture and still need higher titers, you may want to consult with PD-Direct® Services for advice on fed-batch optimization

Up to Double Prior Fed-batch Titers
We have demonstrated 35–120% product titer improvements when FunctionMAX™ TiterEnhancer was added on top of existing processes or as a partial feed replacement (Figures 1–3), resulting in a lower total proportion of feed addition. These increases were achieved primarily by maintaining specific productivity throughout each fed-batch process. Improvements were seen immediately and without process optimization. We have also shown that low-volume addition of FunctionMAX™ TiterEnhancer was sufficient to improve titers for several processes using different commercially available media and feeds, with those having higher cell densities seeing the most benefit. This additive is not designed to completely replace other feeding supplements that provide additional ingredients not found in FunctionMAX™ TiterEnhancer.

Highly Concentrated Ingredients
The technology used to deliver this functional additive promises to transform recombinant drug bioprocessing by raising key ingredient concentrations to levels that are out of reach using conventional approaches. A concentration of 150 g/L is achieved with FunctionMAX™ TiterEnhancer (compared to 20–25 g/L for most basal media), and it has been shown to be stable in dry or liquid format for over 1 year.

Easy-to-Use pH-neutral Format
Reconstitution of the AGT™ dry format is surprisingly simple (add water and stir), with all components going into solution. There is no pH adjustment required, and being pH neutral it is safer in handling and use than extreme pH alternatives. As a pH neutral additive, this product will help avoid bioreactor complications often seen with extreme pH supplements. Further, the liquid is very stable—so much so that the liquid format version is made from the same AGT™ dry format material that is also available for purchase. The liquid is provided for small-scale trials and the AGT™ dry format for seamless scale-up in use.

Specialized Delivery of Ingredients Found in Base Media—Nothing Exotic
FunctionMAX™ TiterEnhancer does not introduce new ingredients to your fed-batch process; it simply provides the same key ingredients found in basal media that the cells need late in culture to maintain their productivity. Further, the technology used to combine these ingredients does not introduce any additional ingredients. Nor does it contain any growth factors or undefined components like hydrolysates. A DMF (drug master file) reference can be requested for this product for assurance of regulatory authorities that there are no substances or ingredients in the formulation that might create regulatory concerns.

Economies in Use
FunctionMAX™ TiterEnhancer is designed to deliver highly concentrated and high value addition to your fed-batch process. Consider that it provides 7-fold the ingredient concentration of a basal medium and can provide up to a doubling of titer. Further, in normal use at about 10% of working volume, the actual price per liter of use is similar to that of basal media.

L-Glutamine (200 mM) Gibco™

L-Glutamine is an amino acid that is required for cell culture. L-Glutamine participates in the formation of purine and pyrimidine nucleotides, amino sugars, glutathione, L-glutamate, and other amino acids, as well as in protein synthesis and glucose production. Unlike most other amino acids, L-glutamine is not stable in solution. The rate at which degradation proceeds is a function of time, temperature, and pH. Gibco® L-glutamine is a ready-to-use 200 mM stock solution that acts as a cell culture supplement. The optimal concentration is dependent upon the cell type and medium used to culture the cells, but generally falls in the range of 2–6 mM.

We also offer Gibco® GlutaMAX™ Supplement as a stable alternative to L-glutamine.

Dual-site cGMP Manufacturing and Quality System
Gibco® L-glutamine is manufactured at a cGMP compliant facility located in Paisley, Scotland, UK. The facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard. For supply chain continuity, we offer an identical Gibco® L-glutamine product made in our Grand Island facility (25030-081). This facility is registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standards.

Feed Kit A+ Gibco™

The Feed Kit A+ provides two chemically defined supplements, GlygcanTune™ A+ Total Feed and EfficientFeed™ A+ Supplement, along with fed-batch supplementation strategies designed to optimize cell growth and protein production while offering the ability to target and fine tune glycosylation profiles. GlycanTune A+ Total Feed is based on the chemically-defined EfficientFeed A+ Supplement and is specifically designed for fed-batch cultures of multiple cell lines. When used alone or with EfficientFeed+ supplement, this single-part total feeding supplement can shift glycan profiles from heavily G0F to mostly G1F and G2F glycans. GlycanTune A+ Total Feed provides an easy-to-reconstitute, pH-neutral solution that can be super-concentrated for delivery of key nutrients at smaller volumes.

Dialing in a glycan profile
The manual included in the kit (and downloadable below) provides complete protocols for testing GlycanTune A+ Total Feed with EfficientFeed A+ Supplement or another feed to obtain a set of conditions similar to those shown in the charts below. The first chart depicts protocol conditions for establishing a glycan shift pattern and the second shows typical results from a shifting experiment to help you hone in on the best feeding strategy to achieve a desired galactosylation profile.

Simplified handling to reduce risk in scale-up
The ability to scale up using AGT dry format provides easier handling and storage in manufacturing and other bioprocess settings. Fewer parts and reconstitution steps mean fewer opportunities for operator error. Further, the ability to store this feed, even when super-concentrated, for the length of a bioreactor run at room temperature in the dark may lessen the chance of contamination by reducing the number of connections to the bioreactor to one or two. Customization of other feeding formulations is possible should that option suit your needs better after trying this kit. Contact your local Field Application Scientist through your Account Manager to learn more about our customization capabilities.

Resurge™ CD4 Supplement, without glucose and L-glutamine Gibco™

Resurge CD4 Supplement, without glucose and L-glutamine, is a chemically defined supplement formulation to complement a variety of base media. This supplement was developed using several host cell lines and in various culture systems, including shaker flasks and bench-scale bioreactors. Resurge CD4 Supplement, without glucose and L-glutamine, was developed for use in CHO mammalian culture systems and can be used either as a media supplement or a culture system feed.

GlycanTune™ C+ Total Feed Gibco™

GlycanTune™ C+ Total Feed is an AGT™, or Advanced Granulation Technology™, feeding supplement specifically designed for a fed-batch culture of multiple cell lines. When used, this dry-format, single-part total feeding supplement can shift glycan profiles from heavily G0F to mostly G1F and G2F glycans. The product is an animal origin–free, chemically-defined formulation that contains no proteins, hydrolysates, or components of unknown composition. GlycanTune C+ Total Feed provides an easy-to-reconstitute, pH-neutral solution that can be concentrated from the suggested 87.7 g/L (1X) up to 175.4 g/L (2X). As a result, key nutrients can be delivered in smaller volumes.

Which GlycanTune Total Feed to choose?

Consider GlycanTune C+ Total Feed when:
• You have used Gibco™ EfficientFeed™ C+ (Plus) AGT Supplement in the past and wish to maintain the benefits of simpler reconstitution and super-concentration in delivering key components alongside improved glycan profile
• You are currently using another feed and wish to shift glycan profile more toward G1 and G2
• You are using a cell line or base medium where Dynamis™ Medium may be considered an option

Consider GlycanTune A+ Total Feed when:
• You have used Gibco EfficientFeed A+ (Plus) AGT Supplement in the past and wish to maintain the benefits of simpler reconstitution and super-concentration in delivering key components alongside improved glycan profile
• You are currently using another feed and wish to shift glycan profile more toward G1 and G2
• You are using a cell line or base medium where CD OptiCHO™ Medium may be considered an option

Consider GlycanTune B+ Total Feed when:
• You have used Gibco EfficientFeed B+ (Plus) AGT Supplement in the past and wish to maintain the benefits of simpler reconstitution and super-concentration in delivering key components alongside improved glycan profile
• You are currently using another feed and wish to shift glycan profile more toward G1 and G2
• You are using a cell line or base medium where CD CHO Medium may be considered an option

Dialing in a glycan profile
By following the protocol recommended to test with GlycanTune Total Feed, it is possible to shift the glycan protein expression profiles from primarily G0 to primarily G1 and G2.

Increasing titers
As a complete feed, GlycanTune total feeds have been shown to provide multiple-fold increases in titers at 1X concentration versus batch culture; as such, they are an excellent starting point for process development when a shift in glycan profile is desired. Flexibility to further increase titers is enhanced with the ability to super-concentrate the product by reconstituting with less water to deliver up to 175.4 g/L of fed nutrients (see figure).

Maximizing bioreactor utilization
Have you ever wished you could have more working volume at the start of a bioreactor run to get more protein produced, but needed too much feeding volume space to accomplish your optimal goal? Have you ever thought that room for a little more feeding might get you to a new level of titer from a process and maybe even reduce the number of bioreactor runs that might be needed per year? In either of these cases, it is important to have the flexibility to super-concentrate your feeding supplements so you get less product dilution, more space for an additional feeding, and/or more working volume to start your run (see figure). In fact, you may gain about 20% more bioreactor utilization by exploring the unique ability to super-concentrate this GlycanTune C+ product.

Simplified handling to reduce risk in scale-up
The ability to scale up using AGT dry format provides easier handling and storage in manufacturing and other bioprocess settings. Fewer parts and reconstitution steps mean fewer opportunities for operator error. Further, the ability to store this feed, even when super-concentrated, for the length of a bioreactor run at room temperature in the dark may allow less chance of contamination by reducing the number of connections to the bioreactor to one or two—depending on whether GlycanTune Total Feed can be used alone or after a change from your current feed.

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