Shop All Gateway Donor Vectors - pDONR

Gateway™ pDONR™/Zeo Vector (Invitrogen™)

Gateway® Technology enables rapid cloning of one or more genes into virtually any protein expression system. Once you have an entry clone, you can recombine your gene of interest into a variety of expression vectors adapted for use in Gateway® Technology. The PCR product is directionally cloned with efficiencies typically >99%. The pDONR™/Zeo vector haa a pUC origin for high plasmid yields and universal M13 sequencing sites for ease of use.

PCR Cloning System with Gateway™ Technology with pDONR™/Zeo & OmniMAX™2 Competent Cells (Invitrogen™)

Gateway® Technology enables rapid cloning of one or more genes into virtually any protein expression system. Once you have an entry clone, you can recombine your gene of interest into a variety of expression vectors adapted for use in Gateway® Technology. The PCR Cloning System with Gateway® Technology is designed to create an entry clone following PCR with attB-modified custom primers (Figure 1). The PCR product is directionally cloned with efficiencies typically >99%. The PCR Cloning System with Gateway® Technology offers:

• Rapid, efficient, directional cloning of PCR products
• Versatility to use any amplification enzyme
• Cloning without the need for restriction endonucleases or ligase
• Selection of entry clones with kanamycin or Zeocin™

In addition, the pDONR™221 and pDONR™/Zeo vectors have a pUC origin for high plasmid yields and universal M13 sequencing sites for ease of use.

PCR Cloning System with Gateway™ Technology with pDONR™221 & OmniMAX™2 Competent Cells (Invitrogen™)

Gateway® Technology enables rapid cloning of one or more genes into virtually any protein expression system. Once you have an entry clone, you can recombine your gene of interest into a variety of expression vectors adapted for use in Gateway® Technology. The PCR Cloning System with Gateway® Technology is designed to create an entry clone following PCR with attB-modified custom primers (Figure 1). The PCR product is directionally cloned with efficiencies typically >99%. The PCR Cloning System with Gateway® Technology offers:

• Rapid, efficient, directional cloning of PCR products
• Versatility to use any amplification enzyme
• Cloning without the need for restriction endonucleases or ligase
• Selection of entry clones with kanamycin or Zeocin™

In addition, the pDONR™221 and pDONR™/Zeo vectors have a pUC origin for high plasmid yields and universal M13 sequencing sites for ease of use.

Gateway™ pDONR™221 Vector (Invitrogen™)

Gateway® Technology enables rapid cloning of one or more genes into virtually any protein expression system. Once you have an entry clone, you can recombine your gene of interest into a variety of expression vectors adapted for use in Gateway® Technology. The PCR product is directionally cloned with efficiencies typically >99%. The pDONR™221 vector has a pUC origin for high plasmid yields and universal M13 sequencing sites for ease of use.