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TaqMan™ Genotyping Master Mix (Applied Biosystems™)

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Alternative Product: Try TaqPath ProAmp Master Mix, our newest and highest performing master mix for genotyping and copy number variation for accurate results, even from samples containing PCR inhibitors. With TaqPath ProAmp Master Mix, we’ve taken the best of TaqMan Genotyping Master Mix and added additional capabilities for your genotyping and copy number variation experiments.

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TaqMan® Genotyping Master Mix is optimized for end-point fluorescence detection in SNP genotyping applications.

• Distinct clusters and high call rates for unambiguous allelic discrimination
• Validated with TaqMan® SNP Genotyping Assays
• Excellent pre- and post-PCR stability for high throughput setup and analysis

May be used for a variety of genotyping applications, including:

• Candidate gene studies
• Drug-target validation
• Disease association studies
• Population genetics
• Linkage mapping
• Agricultural applications

Reaction size is based on a 50 µl reaction volume; please see below for other reaction sizes.

Exceptional Performance from an Optimized Mix (2X)
Accurate genotype assignments result from preferential binding of the allele-specific probe to the matching target. TaqMan® Genotyping Master Mix enables specific binding of the probe to achieve exceptional cluster resolution. Key features include:

• AmpliTaq Gold® DNA Polymerase, UP (Ultra Pure) – Automatic hot start enzyme designed to be active during thermal cycling and inactive at room temperature for easy reaction setup.
• Optimized mix components provide excellent specificity for discrimination between alleles
• Passive internal reference based on proprietary ROX™ dye for increased precision on Applied Biosystems® real-time PCR instruments
• Single thermal cycling condition for consistent results with TaqMan® Assays

Validated with TaqMan® Genotyping Assays and Instruments
TaqMan® Genotyping Master Mix has been tested across all types of TaqMan® SNP Genotyping Assays: TaqMan® Drug Metabolism Genotyping Assays, TaqMan® SNP Genotyping Assays, and Custom TaqMan® SNP Genotyping Assays. In addition, the master mix is validated with the Applied Biosystems® thermal cyclers and real-time PCR systems.

High-Throughput Setup and Analysis
The combination of several low-cost thermal cyclers for PCR and a single real-time PCR instrument for allelic discrimination make high throughput SNP genotyping manageable. TaqMan® Genotyping Master Mix further improves high-throughput setup and analysis with excellent room temperature stability both before and after PCR. This provides the flexibility required to run long experiments, unattended overnight or over a weekend.

Reliable Discrimination with Challenging Targets
Amplicon design constraints make SNP detection challenging, particularly when targets contain an abundance of GC- or AT-rich regions. The optimized components of TaqMan® Genotyping Master Mix result in tight, well-separated clusters and more accurate allele-calls compared to other mixes, even for challenging targets.

Setting the Industry Standard for Quality
Extensive analytical and functional tests are performed for each manufactured lot of TaqMan® Genotyping Master Mix, including one TaqMan® SNP Genotyping Assay and one TaqMan® Drug Metabolism Genotyping Assay. Results are compiled in an informative Certificate of Analysis, which is accessible through the Applied Biosystems® Web site (search for 'Certificate of Analysis').

Number of Genotyping Reactions at a Given Reaction Size:

4371355, TaqMan® Genotyping Master Mix, 1-Pack (1 x 10 mL)
• 50 µL Reactions: 400
• 20 µL Reactions: 1000
• 10 µL Reactions: 2,000
• 5 µL Reactions: 4,000

4381656, TaqMan® Genotyping Master Mix, 2-Pack (2 x 10 mL)
• 50 µL Reactions: 800
• 20 µL Reactions: 2,000
• 10 µL Reactions: 4,000
• 5 µL Reactions: 8,000

4371357, TaqMan® Genotyping Master Mix, (1 x 50 mL)
• 50 µL Reactions: 2000
• 20 µL Reactions: 5,000
• 10 µL Reactions: 10,000
• 5 µL Reactions: 20,000

4381657, TaqMan® Genotyping Master Mix, (2 x 50 mL)
• 50 µL Reactions: 4000
• 20 µL Reactions: 10,000
• 10 µL Reactions: 20,000
• 5 µL Reactions: 40,000

Note: See user's manual or package insert for limited label license, and trademark information. For Research Use Only. Not for use in diagnostics procedures.

QuantStudio™ 12K Flex OpenArray™ Practice Kit (Applied Biosystems™)

The QuantStudio™ 12K Flex OpenArray® Practice Kit is designed to introduce newcomers to the OpenArray® block workflow, as well as to provide instrument verification. The kit contains 6 practice loading arrays plus reagents. The practice kit also includes these accessories: 6 lids and plugs, 6 immersion fluid syringes, 2 carriers, 1 box of Accufill™tips, and 384-well plates.

TaqMan™ Sample-to-SNP™ Kit (20 mL sample prep, 10 mL PCR) (Applied Biosystems™)

The Applied Biosystems® TaqMan® Sample-to-SNP™ Kit allows you to go from Biological Sample to Genotyping Results in less than one hour. The TaqMan® Sample-to-SNP™ Kit combines DNA extraction and TaqMan® chemistry-based genotyping in a streamlined protocol to provide results from any sample in less than one hour.

• DNA Extract All Reagents kits provide PCR-ready DNA from a wide variety of sample types ranging from blood to buccal swabs to plant tissues in 5 min
• The TaqMan®™ GTXpress™ Master Mix provides robust PCR amplification of extracted DNA in less than 50 min
• Compatible with both Fast and Standard mode thermal cycling conditions
• Pre-mixed components stored at 2°–8°C significantly reduce assay setup time
• Validated with all types of Applied Biosystems® TaqMan® Genotyping Assays
• Extended PCR cycling for poorly amplified or sample-limited reactions

The kit includes reagents sufficient for 400 sample preparation reactions at 50 µL/ reaction and 4,000 PCR reactions at 5 µL for each reaction.

Please note:
The DNA Extract All reagents are not available for individual sale. They are only available as a part of the TaqMan® Sample-to-SNP™ Kit.

TaqMan™ Sample-to-SNP™ Kit (20 mL sample prep, 50 mL PCR) (Applied Biosystems™)

The Applied Biosystems® TaqMan® Sample-to-SNP™ Kit allows you to go from Biological Sample to Genotyping Results in less than one hour. The TaqMan® Sample-to-SNP™ Kit combines DNA extraction and TaqMan® chemistry-based genotyping in a streamlined protocol to provide results from any sample in less than one hour.

• DNA Extract All Reagents kits provide PCR-ready DNA from a wide variety of sample types ranging from blood to buccal swabs to plant tissues in 5 min
• The TaqMan®™ GTXpress™ Master Mix provides robust PCR amplification of extracted DNA in less than 50 min
• Compatible with both Fast and Standard mode thermal cycling conditions
• Pre-mixed components stored at 2°–8°C significantly reduce assay setup time
• Validated with all types of Applied Biosystems® TaqMan® Genotyping Assays
• Extended PCR cycling for poorly amplified or sample-limited reactions

The kit includes reagents sufficient for 400 sample preparation reactions at 50 µL/ reaction and 20,000 PCR reactions at 5 µL for each reaction.

Please note:
The DNA Extract All reagents are not available for individual sale. They are only available as a part of the TaqMan® Sample-to-SNP™ Kit.

MeltDoctor™ HRM Reagent Kit (Applied Biosystems™)

Applied Biosystems® MeltDoctor™ HRM Reagent Kit provides individually packaged PCR components and the MeltDoctor™ HRM Dye required for high resolution melting analysis. High Resolution Melting (HRM) analysis is a post-PCR analysis method used to identify genetic variation in nucleic acid sequences.
This kit includes:
• AmpliTaq Gold® 360 DNA Polymerase which, when combined with AmpliTaq Gold® 360 Buffer and 360 GC Enhancer, amplifies a vast range of DNA sequence contexts. AmpliTaq Gold® 360 DNA Polymerase delivers 360° coverage for a full range of targets.
• GeneAmp® dNTP Blend
• MeltDoctor™ HRM Dye, a stabilized form of the fluorescent SYTO® 9 double-stranded nucleic acid stain developed by Molecular Probes

Luminaris Color HRM qPCR Master Mix (Thermo Scientific™)

Thermo Scientific Luminaris Color HRM Master Mix is a convenient, ready-to-use master mix designed for the accurate detection of DNA sequence variations using High Resolution Melt (HRM) analysis method. The Master Mix complements Thermo Scientific PikoReal Real-Time System and software to provide a complete solution for HRM analysis.

The Luminaris Color HRM Master Mix contains Hot Start Taq DNA polymerase in an optimized buffer system with EvaGreen™ fluorescent dye for robust and reliable performance in PCR and HRM. The chemically modified Hot Start Taq DNA polymerase has ultra low residual activity until thermally activated ensuring PCR specificity and sensitivity. The DNA binding dye EvaGreen™ enables discrimination of even the most challenging sequence differences (such as Type IV SNP) without PCR inhibition and with no apparent sequence preference. The Luminaris Color HRM Master Mix is supplemented with an inert blue dye and a separate Yellow Sample Buffer that contains a yellow dye. Mixing both components in a reaction turns the solution green. This provides a visual aid when pipetting and decreases the risk of errors during reaction setup, especially when using white reaction vessels.

Highlights

• High performance on most real-time instruments with HRM capabilities
• Dual-color system for easy reaction setup
• Superior discrimination of all four SNP classes
• High sensitivity—accurate results from 30 copies of genomic DNA
• Exceptional stability of fully assembled reaction—for high-throughput applications
• Optimized for PikoReal Real-Time System and software

Applications

• SNP genotyping
• Mutation discovery (gene scanning)
• Heterozygosity screening
• Bacterial genotyping
• CpG methylation analysis

Includes
• Luminaris Color HRM Master Mix (2X) includes chemically modified Hot Start Taq DNA Polymerase, EvaGreen™ fluorescent dye, dNTPs and MgCl2 in an optimized PCR buffer with a blue dye.
• 40x Yellow Sample Buffer with a yellow dye.
• Nuclease-free water.

Related Products
Luminaris Color HRM qPCR Master Mix

SNaPshot™ Multiplex Kit (Applied Biosystems™)

The SNaPshot® Multiplex System is a primer extension-based method that enables multiplexing up to 10 SNPs (single nucleotide polymorphisms). Use this system to screen and confirm SNPs, assess DNA methylation, fingerprint BACs, and screen samples for susceptibility to scrapie.

• Perform multiplexed, single-base extension of up to 10 SNPs.
• Interrogate multiple SNPs regardless of chromosome position.
• Separate SNP loci that differ by a single base pair.
• Perform robust interrogation of multiple SNPs with only a small amount of amplified template.
• Decrease expenses with this moderately priced system.
• Reduce the complexity of your experiments with this well-tested, easy-to-understand single-base extension technique.

Screen and Confirm SNPs
The SNaPshot® Multiplex System is the perfect tool for SNP screening and validation. The kit offers a one-tube single-base extension⁄termination reagent to label DNA fragments. First, run DNA fragments on any Applied Biosystems® capillary electrophoresis instrument with the GeneScan™-120 LIZ® Size Standard to determine the size of labeled fragments. Then use GeneMapper® Software to analyze the data and generate allele calls. The SNaPshot® Multiplex Kit allows multiplexing during single-base extension of up to 10 primer-template combinations in a single tube-single capillary format.

Assess DNA Methylation
The study of methylation⁄epigenetics is emerging as an important component of cancer research. In a typical assay to detect methylation, bisulfite treatment of DNA deaminates non-methylated cytosine and converts it to uracil while methylated cytosine remains unreactive. The subsequent step of PCR amplification coverts uracil bases to thymine. Use SNaPshot® to quantitatively detect the base differences in treated and untreated samples to learn the methylation status of your samples.

Fingerprint BACs
New BAC libraries require a rapid, efficient method for characterization (also called fingerprinting) and assembly of clones into contigs (contiguous consensus sequences), which are then arrayed into physical maps of the chromosome. Use the SNaPshot® Multiplex Kit to fingerprint clones by labeling BAC fragments after digestion with restriction endonucleases. The labeled fragments can then be separated and detected on any Applied Biosystems® capillary electrophoresis instrument. Sizing information from GeneMapper® Software v3.5 or higher is imported into subsequent editing and contig assembly programs. The high-quality results you get from the SNaPshot® Multiplex Kit provide an easy-to-use and cost-effective solution for high-throughput BAC fingerprinting.

Screen Samples for Susceptibility to Scrapie
Scrapie, like other prion diseases are caused by abnormally-folded isoforms of host-encoded proteins. The single base-pair sensitivity of the SNaPshot® system enables you to screen samples accurately for codon differences in prion genes. Use the SNaPshot® Multiplex System to screen for SNPs in the genes that code for these proteins. For instance, polymorphisms at codons 136, 154 and 171 of the PrP gene in sheep and goats can lead to abnormally folded isoforms of the protein product to result in scrapie.

For Research Use Only. Not for use in diagnostics procedures.

TaqMan™ GTXpress™ Master Mix (Applied Biosystems™)

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Alternative Product: Try TaqPath ProAmp Master Mix, our newest and highest performing genotyping master mix for accurate results, even from samples containing PCR inhibitors. With TaqPath ProAmp Master Mix, we’ve taken the best of TaqMan GTXpress Master Mix and added additional capabilities for your genotyping experiments.

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TaqMan® GTXpress™ Master Mix delivers accurate genotyping results with robust performance in less than 50 min. Applied Biosystems® TaqMan® GTXpress™ Master Mix (2X) combines AmpliTaq® Fast DNA Polymerase, UP, dNTPs, Tracking Dye, and ROX™ dye in a convenient single vial format. The 1 mL pack includes reagents sufficient to perform 400 PCR reactions (5 µL/reaction).
• Compatible with both Fast and Standard mode thermal cycling conditions
• Pre-mixed components stored at 2°–8°C significantly reduce assay setup time
• Validated with all types of Applied Biosystems® TaqMan® Genotyping Assays
• Extended PCR cycling can be used for poorly amplified or sample-limited reactions The TaqMan® GTXpress™ Master Mix is also sold as part of TaqMan® Sample-to-SNP™ Kit, which provides real-time PCR-ready DNA in 5 min from a wide variety of samples, ranging from blood to buccal swabs to plant tissues. For more information on the TaqMan® Sample-to-SNP™ Kit, refer to the Applied Biosystems® TaqMan® Sample-to-SNP™ Kit Protocol (PN. 4402136).

Bovine Genotypes Panel 3.1 Kit (Thermo Scientific™)

Thermo Scientific Bovine Genotypes Panel 3.1 Kit is offered for cattle parentage testing and identification using Short Tandem Repeat (STR) DNA loci.

The Bovine Genotypes Panel 3.1 allows co-amplification of the 18 microsatellites in a single multiplex PCR reaction. One primer from each primer pair is end-labeled with a fluorescent dye. After PCR, the fragments are separated and detected in a single electrophoresis injection, using an automated electrophoresis instrument (see Figure 2). In addition, the kit includes bovine control DNA for verification of acceptable PCR and electrophoresis conditions. Reagents and protocols of the Bovine Genotypes Panel 3.1 have been optimized to deliver similar peak sizes (amplification yields) for each loci. Allele callings obtained with this kit represent the true alleles of an individual, instead of 'plus-A peaks' or 'split peaks' typically interpreted when using e.g. a conventional Taq DNA polymerase. The kit employs Phusion Hot Start DNA Polymerase that has a proofreading activity and eliminates the "plus-A peaks".

Thermo Scientific Bovine Genotypes Panel 3.1 encompasses all the 12 STR loci recommended by the International Society for Animal Genetics (ISAG) for routine use in parentage testing and identification, including TGLA227, BM2113, TGLA53, ETH10, SPS115, TGLA126, TGLA122, INRA23, ETH3, ETH225, BM1824, and BM1818. In addition, the kit includes the following six microsatellites, which are among the list of loci recommended by the Food and Agriculture Organization of the United Nations (FAO) for genetic studies of domestic animals: SPS113, RM067, CSRM60, MGTG4B, CSSM66, and ILSTS006. Bovine Genotypes Panel 3.1 is a combination of Panels 1.2 and 2.2, thus enabling the analysis of both panels in a single reaction.

Highlights

• Various kit choices that include STR loci recommended by ISAG
• Single multiplex reaction - less pipetting steps
• Optimized reagents
• Control DNA provided for verification of PCR and electrophoresis conditions
• Allele calling represents true alleles (no plus-A peaks)

This product has been developed in collaboration with Maxxam Analytics Inc.

Bovine Genotypes Panel 2.2 Kit (Thermo Scientific™)

Thermo Scientific Bovine Genotypes Panel 2.2 Kit is offered for cattle parentage testing and identification using Short Tandem Repeat (STR) DNA loci.

The Bovine Genotypes Panel 2.2 allows co-amplification of the 6 microsatellites in a single multiplex PCR reaction. One primer from each primer pair is end-labeled with a fluorescent dye. After PCR, the fragments are separated and detected in a single electrophoresis injection, using an automated electrophoresis instrument (see Figure 2). In addition, the kit includes bovine control DNA for verification of acceptable PCR and electrophoresis conditions. Reagents and protocols of the Bovine Genotypes Panel 2.2 have been optimized to deliver similar peak sizes (amplification yields) for each loci. Allele callings obtained with this kit represent the true alleles of an individual, instead of 'plus-A peaks' or 'split peaks' typically interpreted when using e.g. a conventional Taq DNA polymerase. The kit employs Phusion Hot Start DNA Polymerase that has a proofreading activity and eliminates the "plus-A peaks".

Thermo Scientific Bovine Genotypes Panel 2.2 encompasses the following six STR loci: SPS113, RM067, CSRM60, MGTG4B, CSSM66, and ILSTS006. These are among the list of loci recommended by the International Society for Animal Genetics (ISAG) and the Food and Agriculture Organization of the United Nations (FAO) for genetic studies of domestic animals.

The Bovine Genotypes Panel 2.2 is intended to be used as a complement to the Bovine Genotypes Panel 1.2 when more STR loci are required for resolving a parentage case. The microsatellite alleles of the Bovine Genotypes Panel 2.2 kit can be separated and detected in the same single electrophoresis injection with the microsatellite alleles of the Bovine Genotypes Panel 1.2 kit (see Figure 2).

Highlights

• Various kit choices that include STR loci recommended by ISAG
• Single multiplex reaction - less pipetting steps
• Optimized reagents
• Control DNA provided for verification of PCR and electrophoresis conditions
• Allele calling represents true alleles (no plus-A peaks)

This product has been developed in collaboration with Maxxam Analytics Inc.

MeltDoctor™ HRM Master Mix (Applied Biosystems™)

Applied Biosystems® MeltDoctor™ HRM Master Mix contains all components (excluding template and primers) formulated for superior HRM performance across a wide range of genomic targets. High Resolution Melting (HRM) analysis is a post-PCR analysis method used to identify genetic variation in nucleic acid sequences. Unlike some competitive mixes, the MeltDoctor™ HRM Master Mix does not require additional mixing prior to use, and was developed and optimized solely for HRM applications.
• Low background fluorescence
• High brightness in the presence of double-stranded DNA
• Minimal temperature shift of DNA melting due to dye binding
• Thermal stability to tolerate PCR cycling conditions
• No inhibition of polymerase activity, resulting in high PCR efficiency
• A dNTP blend including dUTP, which minimizes carryover contamination by allowing amplicon degradation by uracil DNA glycosylase (UDG) in subsequent PCR reactions

Perform Mutation Scanning
HRM analysis can be used to scan for mutations in target genes for the identification of variant samples prior to sequencing analysis. As a mutation scanning technique, HRM offers significant advantages over conventional methods such as Denaturing High- Performance Liquid Chromatography (DHPLC) and Denaturing Gradient Gel Electrophoresis (DGGE). Specifically, the advantages of HRM for mutation scanning include:
• Low reagent consumption, with little waste: HRM requires only a 20 µL PCR reaction for analysis of each sample, eliminating the need for HPLC solvents or DGGE gels
• Simple, fast workflow: no additional instrumentation is required after PCR amplification. A high-resolution melt step can be simply added to the end of the PCR profile for immediate analysis
• Fast optimization: unlike DHPLC, thermal optimization is not required
• Low sample consumption: following HRM analysis, the PCR product can be used directly in a Sanger sequencing reaction

Quality Components
• Magnesium salts and other buffer components, precisely formulated to obtain optimal HRM results.
• AmpliTaq Gold® 360 DNA Polymerase, a highly purified DNA polymerase that provides hot-start performance, minimizing non-specific product formation and enabling reactions to be set up at room temperature
• MeltDoctor™ HRM Dye, a stabilized form of the fluorescent SYTO® 9 double-stranded nucleic acid stain developed by Molecular Probes.

TaqMan™ Sample-to-SNP™ Kit (5 mL sample prep, 1 mL PCR) (Applied Biosystems™)

The Applied Biosystems® TaqMan® Sample-to-SNP™ Kit allows you to go from Biological Sample to Genotyping Results in less than one hour. The TaqMan® Sample-to-SNP™ Kit combines DNA extraction and TaqMan® chemistry-based genotyping in a streamlined protocol to provide results from any sample in less than one hour.

• DNA Extract All Reagents kits provide PCR-ready DNA from a wide variety of sample types ranging from blood to buccal swabs to plant tissues in 5 min
• The TaqMan®™ GTXpress™ Master Mix provides robust PCR amplification of extracted DNA in less than 50 min
• Compatible with both Fast and Standard mode thermal cycling conditions
• Pre-mixed components stored at 2°–8°C significantly reduce assay setup time
• Validated with all types of Applied Biosystems® TaqMan® Genotyping Assays
• Extended PCR cycling for poorly amplified or sample-limited reactions

The kit includes reagents sufficient for 100 sample preparation reactions at 50 µL/ reaction and 400 PCR reactions at 5 µL for each reaction.

Please note:
The DNA Extract All reagents are not available for individual sale. They are only available as a part of the TaqMan® Sample-to-SNP™ Kit.

Canine Genotypes Panel 1.1 Kit (Thermo Scientific™)

Thermo Scientific Canine Genotypes Panel 1.1 is a reagents kit for canine parentage testings and identification using Short Tandem Repeat (STR) DNA loci. Due to their high level of informativeness and Mendelian inheritance, microsatellites have become the markers of choice for parentage testing, identification and forensics.

The Canine Genotypes Panel 1.1 provides all the necessary reagents for amplification of the 19 microsatellite loci. The kit contains master mix and primer mix allows co-amplification of the microsatellites in a single multiplex PCR reaction. Canine control DNA is provided to verify acceptable PCR and electrophoresis conditions. Reagents and protocols of the Canine Genotypes Panel 1.1 kit have been optimized to deliver similar peak sizes (amplification yields) for each loci. Allele callings obtained with this kit represent the true alleles of an individual, instead of 'plus-A peaks' or 'split peaks' typically interpreted when using e.g. a conventional Taq DNA polymerase. The kit employs Phusion Hot Start DNA Polymerase that has a proofreading activity and eliminates the "plus-A peaks". One primer from each primer pair is end-labelled with a fluorescent dye. After PCR, the fragments are separated and detected in a single electrophoresis injection, using an automated capillary electrophoresis instrument.

Canine Genotypes Panel 1.1 encompasses the following 19 loci: AHTk211, CXX279, REN169O18, INU055, REN54P11, INRA21, AHT137, REN169D01, AHTh260, AHTk253, INU005, INU030, FH2848, AHT121, FH2054, REN162C04, AHTh171, REN247M23 and Amelogenin (sex determining locus). These markers are included in the 'core panel' of loci recommended by the Applied Genetics Committee of Companying Animals of the International Society for Animal Genetics (ISAG).

Highlights

• Single multiplex PCR of 19 STR loci
• Master mix and Primer mix provided for easy pipetting
• Control DNA provided for verification of PCR and electrophoresis conditions
• Allele calls represents true alleles (no plus-A peaks)

Bovine Genotypes Panel 1.2 Kit (Thermo Scientific™)

Thermo Scientific Bovine Genotypes Panel 1.2 kit is offered for cattle parentage testing and identification using Short Tandem Repeat (STR) DNA loci.

The Bovine Genotypes Panel 1.2 allows co-amplification of the 12 microsatellites in a single multiplex PCR reaction. One primer from each primer pair is end-labeled with a fluorescent dye. After PCR, the fragments are separated and detected in a single electrophoresis injection, using an automated electrophoresis instrument (see Figure 1). In addition, the kit includes bovine control DNA for verification of acceptable PCR and electrophoresis conditions. Reagents and protocols of the Bovine Genotypes Panel 1.2 have been optimized to deliver similar peak sizes (amplification yields) for each loci. Allele callings obtained with this kit represent the true alleles of an individual, instead of 'plus-A peaks' or 'split peaks' typically interpreted when using e.g. a conventional Taq DNA polymerase. The kit employs Phusion Hot Start DNA Polymerase that has a proofreading activity and eliminates the "plus-A peaks".

Thermo Scientific Bovine Genotypes Panel 1.2 encompasses all the 12 STR loci recommended by the International Society for Animal Genetics (ISAG) for routine use in bovine parentage testing and identification, including: TGLA227, BM2113, TGLA53, ETH10, SPS115, TGLA126, TGLA122, INRA23, BM1818, ETH3, ETH225, and BM1824. The primer sequences employed by the kit are identical to those recommended by the Cattle Molecular Markers and Parentage Testing standing committee of ISAG, and they have been validated in extensive laboratory comparison tests.

Highlights

• Various kit choices that include STR loci recommended by ISAG
• Single multiplex reaction - less pipetting steps
• Optimized reagents
• Control DNA provided for verification of PCR and electrophoresis conditions
• Allele calling represents true alleles (no plus-A peaks)

OncoScan™ CNV Plus Reagent Kit (Applied Biosystems™)

The OncoScan CNV Plus Reagent Kit is part of the OncoScan CNV Plus Assay and so is used in conjunction with OncoScan CNV Plus arrays. The OncoScan CNV Plus Reagent Kit contains somatic mutation probe mix, copy number probe mix and controls, gap fill and 1st stage PCR reagents, 2nd stage PCR and post PCR processing reagents, stain reagents, and wash buffers.