Shop All Genotyping Kits

OncoScan™ CNV Plus Reagent Kit Applied Biosystems™

The OncoScan CNV Plus Reagent Kit is part of the OncoScan CNV Plus Assay and so is used in conjunction with OncoScan CNV Plus arrays. The OncoScan CNV Plus Reagent Kit contains somatic mutation probe mix, copy number probe mix and controls, gap fill and 1st stage PCR reagents, 2nd stage PCR and post PCR processing reagents, stain reagents, and wash buffers.

TaqMan™ Sample-to-SNP™ Kit (200 mL sample prep, 10 mL PCR) Applied Biosystems™

The Applied Biosystems® TaqMan® Sample-to-SNP™ Kit allows you to go from Biological Sample to Genotyping Results in less than one hr. The TaqMan® Sample-to-SNP™ Kit combines DNA extraction and TaqMan® chemistry-based genotyping in a streamlined protocol to provide results from any sample in less than one hour.

• DNA Extract All Reagents kits provide PCR-ready DNA from a wide variety of sample types ranging from blood to buccal swabs to plant tissues in 5 min
• The TaqMan®™ GTXpress™ Master Mix provides robust PCR amplification of extracted DNA in less than 50 min
• Compatible with both Fast and Standard mode thermal cycling conditions
• Pre-mixed components stored at 2°–8°C significantly reduce assay setup time
• Validated with all types of Applied Biosystems® TaqMan® Genotyping Assays
• Extended PCR cycling for poorly amplified or sample-limited reactions

The kit includes reagents sufficient for 4,000 sample preparation reactions at 50 µL/ reaction and 4,000 PCR reactions at 5 µL for each reaction.

Please note:
The DNA Extract All reagents are not available for individual sale. They are only available as a part of the TaqMan® Sample-to-SNP™ Kit.

TaqMan™ Sample-to-SNP™ Kit (20 mL sample prep, 10 mL PCR) Applied Biosystems™

The Applied Biosystems® TaqMan® Sample-to-SNP™ Kit allows you to go from Biological Sample to Genotyping Results in less than one hour. The TaqMan® Sample-to-SNP™ Kit combines DNA extraction and TaqMan® chemistry-based genotyping in a streamlined protocol to provide results from any sample in less than one hour.

• DNA Extract All Reagents kits provide PCR-ready DNA from a wide variety of sample types ranging from blood to buccal swabs to plant tissues in 5 min
• The TaqMan®™ GTXpress™ Master Mix provides robust PCR amplification of extracted DNA in less than 50 min
• Compatible with both Fast and Standard mode thermal cycling conditions
• Pre-mixed components stored at 2°–8°C significantly reduce assay setup time
• Validated with all types of Applied Biosystems® TaqMan® Genotyping Assays
• Extended PCR cycling for poorly amplified or sample-limited reactions

The kit includes reagents sufficient for 400 sample preparation reactions at 50 µL/ reaction and 4,000 PCR reactions at 5 µL for each reaction.

Please note:
The DNA Extract All reagents are not available for individual sale. They are only available as a part of the TaqMan® Sample-to-SNP™ Kit.

TaqMan™ Genotyping Master Mix Applied Biosystems™

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Alternative Product: Try TaqPath ProAmp Master Mix, our newest and highest performing master mix for genotyping and copy number variation for accurate results, even from samples containing PCR inhibitors. With TaqPath ProAmp Master Mix, we’ve taken the best of TaqMan Genotyping Master Mix and added additional capabilities for your genotyping and copy number variation experiments.

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TaqMan® Genotyping Master Mix is optimized for end-point fluorescence detection in SNP genotyping applications.

• Distinct clusters and high call rates for unambiguous allelic discrimination
• Validated with TaqMan® SNP Genotyping Assays
• Excellent pre- and post-PCR stability for high throughput setup and analysis

May be used for a variety of genotyping applications, including:

• Candidate gene studies
• Drug-target validation
• Disease association studies
• Population genetics
• Linkage mapping
• Agricultural applications

Reaction size is based on a 50 µl reaction volume; please see below for other reaction sizes.

Exceptional Performance from an Optimized Mix (2X)
Accurate genotype assignments result from preferential binding of the allele-specific probe to the matching target. TaqMan® Genotyping Master Mix enables specific binding of the probe to achieve exceptional cluster resolution. Key features include:

• AmpliTaq Gold® DNA Polymerase, UP (Ultra Pure) – Automatic hot start enzyme designed to be active during thermal cycling and inactive at room temperature for easy reaction setup.
• Optimized mix components provide excellent specificity for discrimination between alleles
• Passive internal reference based on proprietary ROX™ dye for increased precision on Applied Biosystems® real-time PCR instruments
• Single thermal cycling condition for consistent results with TaqMan® Assays

Validated with TaqMan® Genotyping Assays and Instruments
TaqMan® Genotyping Master Mix has been tested across all types of TaqMan® SNP Genotyping Assays: TaqMan® Drug Metabolism Genotyping Assays, TaqMan® SNP Genotyping Assays, and Custom TaqMan® SNP Genotyping Assays. In addition, the master mix is validated with the Applied Biosystems® thermal cyclers and real-time PCR systems.

High-Throughput Setup and Analysis
The combination of several low-cost thermal cyclers for PCR and a single real-time PCR instrument for allelic discrimination make high throughput SNP genotyping manageable. TaqMan® Genotyping Master Mix further improves high-throughput setup and analysis with excellent room temperature stability both before and after PCR. This provides the flexibility required to run long experiments, unattended overnight or over a weekend.

Reliable Discrimination with Challenging Targets
Amplicon design constraints make SNP detection challenging, particularly when targets contain an abundance of GC- or AT-rich regions. The optimized components of TaqMan® Genotyping Master Mix result in tight, well-separated clusters and more accurate allele-calls compared to other mixes, even for challenging targets.

Setting the Industry Standard for Quality
Extensive analytical and functional tests are performed for each manufactured lot of TaqMan® Genotyping Master Mix, including one TaqMan® SNP Genotyping Assay and one TaqMan® Drug Metabolism Genotyping Assay. Results are compiled in an informative Certificate of Analysis, which is accessible through the Applied Biosystems® Web site (search for 'Certificate of Analysis').

Number of Genotyping Reactions at a Given Reaction Size:

4371355, TaqMan® Genotyping Master Mix, 1-Pack (1 x 10 mL)
• 50 µL Reactions: 400
• 20 µL Reactions: 1000
• 10 µL Reactions: 2,000
• 5 µL Reactions: 4,000

4381656, TaqMan® Genotyping Master Mix, 2-Pack (2 x 10 mL)
• 50 µL Reactions: 800
• 20 µL Reactions: 2,000
• 10 µL Reactions: 4,000
• 5 µL Reactions: 8,000

4371357, TaqMan® Genotyping Master Mix, (1 x 50 mL)
• 50 µL Reactions: 2000
• 20 µL Reactions: 5,000
• 10 µL Reactions: 10,000
• 5 µL Reactions: 20,000

4381657, TaqMan® Genotyping Master Mix, (2 x 50 mL)
• 50 µL Reactions: 4000
• 20 µL Reactions: 10,000
• 10 µL Reactions: 20,000
• 5 µL Reactions: 40,000

Note: See user's manual or package insert for limited label license, and trademark information. For Research Use Only. Not for use in diagnostics procedures.

TaqMan™ Sample-to-SNP™ Kit (20 mL sample prep, 50 mL PCR) Applied Biosystems™

The Applied Biosystems® TaqMan® Sample-to-SNP™ Kit allows you to go from Biological Sample to Genotyping Results in less than one hour. The TaqMan® Sample-to-SNP™ Kit combines DNA extraction and TaqMan® chemistry-based genotyping in a streamlined protocol to provide results from any sample in less than one hour.

• DNA Extract All Reagents kits provide PCR-ready DNA from a wide variety of sample types ranging from blood to buccal swabs to plant tissues in 5 min
• The TaqMan®™ GTXpress™ Master Mix provides robust PCR amplification of extracted DNA in less than 50 min
• Compatible with both Fast and Standard mode thermal cycling conditions
• Pre-mixed components stored at 2°–8°C significantly reduce assay setup time
• Validated with all types of Applied Biosystems® TaqMan® Genotyping Assays
• Extended PCR cycling for poorly amplified or sample-limited reactions

The kit includes reagents sufficient for 400 sample preparation reactions at 50 µL/ reaction and 20,000 PCR reactions at 5 µL for each reaction.

Please note:
The DNA Extract All reagents are not available for individual sale. They are only available as a part of the TaqMan® Sample-to-SNP™ Kit.

SNaPshot™ Multiplex Kit Applied Biosystems™

The SNaPshot® Multiplex Kit is part of the SNaPshot® Multiplex System. The SNaPshot® Multiplex System primer extension-based method enables multiplexing up to 10 single nucleotide polymorphisms (SNPs). Use this system to screen and confirm SNPs, detect minor sequence variations, assess DNA methylation, fingerprint bacterial artificial chromosomes (BACs) or general labeling of restriction fragments.

• Interrogate up to 10 SNPs from different amplicons in a single base extension reaction
• Separate SNP loci that differ by a single base pair
• Increased sensitivity compared to standard sequencing allows detection of minor variants (e.g. somatic mutations) in a test sample
• Reduce the complexity of your experiments with this easy to use and cost-efficient genotyping system
• A large body of scientifc literature demonstrates the utility for genotyping, mutation detection and mapping

The SNaPshot® Multiplex Kit is a part of the SNaPshot® Multiplex System:
1) SNaPshot® Multiplex Kit (100, 1000, 5000 reaction sizes)
2) GeneScan™-120 LIZ™ Size Standard
3) Matrix Standard Set DS-02 for dye set E5 appropriate for your instrument

Each of the three components above is required to run the SNaPshot® Multiplex Kit. The Matrix Standard set, however, is only required at a one-time initial set up to calibrate the instrument for the SNaPshot-specific dye set E5.

4) GeneMapper® Software v.4.0 (or higher version) is the recommended secondary data analysis software that enables set up of user-defined panels and bins for automated data analysis.

Alternatively, simple, manual peak analysis is possible using PeakScanner™ 1.0 software (free download from website) for the 310, 3100. 3130, and 3730 instruments (PeakScanner™ is not compatible with data from 3500 instruments). For the 3500 instruments the primary data analysis software on the instrument allows fragment sizing and color separation similar to PeakScanner™.

5) The SNaPshot primer Focus Kit (4329538) is a useful accessory kit for single experiment or assay (panel) design: Analyze individual primers for their approximate sizing location. This will determine whether or not two oligonucleotides will produce overlapping signals when run simultaneously and enable the setting of proper loci windows (bins) for GeneMapper analysis software.

Screen and Confirm SNPs
The SNaPshot® Multiplex System is the perfect tool for SNP screening and validation. The kit offers a one-tube single-base extension⁄termination reagent to label DNA fragments. Run DNA fragments on any Applied Biosystems® capillary electrophoresis instrument with the GeneScan™-120 LIZ® Size Standard to indicate the size of labeled fragments. Then use GeneMapper® Software to analyze the data and generate allele calls. The SNaPshot® Multiplex Kit allows multiplexing during single-base extension of up to 10 primer-template combinations in a single tube-single capillary format.

Assess DNA Methylation
The study of methylation⁄epigenetics is emerging as an important component of cancer research. In a typical assay to detect methylation, bisulfite treatment of DNA deaminates non-methylated cytosine and converts it to uracil while methylated cytosine remains unreactive. The subsequent step of PCR amplification coverts uracil bases to thymine. Use SNaPshot® todetect the base differences in treated and untreated samples to learn the methylation status of your samples.

Fingerprint BACs
New BAC libraries require a rapid, efficient method for characterization (also called fingerprinting) and assembly of clones into contigs (contiguous consensus sequences), which are then arrayed into physical maps of the chromosome. Use the SNaPshot® Multiplex Kit to fingerprint clones by labeling BAC fragments after digestion with restriction endonucleases. The labeled fragments can then be separated and detected on any Applied Biosystems® capillary electrophoresis instrument. Sizing information from GeneMapper® Software v 4.0 or higher is imported into subsequent editing and contig assembly programs.

In a similar fashion, any restriction fragment with a 5' overhang can be labeled with a fluorescent dye using the SNaPshot® reagent.

For Research Use Only. Not for use in diagnostics procedures.

CLA GlobalFiler™ PCR Amplification Kit Applied Biosystems™

The CLA (Cell Line Authentication) GlobalFiler PCR Amplification Kit simplifies the amplification step during authentication of cell lines. GlobalFiler PCR amplification kits are 6-dye, 24-locus short tandem repeat (STR) analysis kits that combine maximum compatibility with global databasing loci standards with dramatically reduced amplification time and superior discrimination power, enabling labs with improved overall efficiency. GlobalFiler kits are backed by best-in-class global training, service, and support. This kit includes GeneScan 600 LIZ Size Standard v2.0, a five-dye-labeled, high-density size standard for the reproducible sizing of fragment analysis data.

Features of the CLA GlobalFiler PCR Amplification Kit include:
• 6-dye, 24-locus STR analysis kit compatible with all cell line databasing loci standards
• Maximum compatibility with global databasing loci standards
• Reduced amplification time and superior discrimination power
• Size standard included for ease of purchase
• Compatible with SeqStudio, 3730/xL, and 3500/xL genetic analyzers

Cell line authentication using short tandem repeat (STR) analysis kits can be used both for human sample authentication (HSA) and mixed sample analysis (MSA). In HSA, there is a need to verify the DNA profile of the sample to check for sample cross-contamination or sample mix-up, or to perform a quality check. For MSA there is a need to deconvolute the presence of multiple contributors in a sample (chimerism) through identification of multiple DNA genotypes in the sample.

TaqMan™ GTXpress™ Master Mix Applied Biosystems™

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Alternative Product: Try TaqPath ProAmp Master Mix, our newest and highest performing genotyping master mix for accurate results, even from samples containing PCR inhibitors. With TaqPath ProAmp Master Mix, we’ve taken the best of TaqMan GTXpress Master Mix and added additional capabilities for your genotyping experiments.

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TaqMan® GTXpress™ Master Mix delivers accurate genotyping results with robust performance in less than 50 min. Applied Biosystems® TaqMan® GTXpress™ Master Mix (2X) combines AmpliTaq® Fast DNA Polymerase, UP, dNTPs, Tracking Dye, and ROX™ dye in a convenient single vial format. The 1 mL pack includes reagents sufficient to perform 400 PCR reactions (5 µL/reaction).
• Compatible with both Fast and Standard mode thermal cycling conditions
• Pre-mixed components stored at 2°–8°C significantly reduce assay setup time
• Validated with all types of Applied Biosystems® TaqMan® Genotyping Assays
• Extended PCR cycling can be used for poorly amplified or sample-limited reactions The TaqMan® GTXpress™ Master Mix is also sold as part of TaqMan® Sample-to-SNP™ Kit, which provides real-time PCR-ready DNA in 5 min from a wide variety of samples, ranging from blood to buccal swabs to plant tissues. For more information on the TaqMan® Sample-to-SNP™ Kit, refer to the Applied Biosystems® TaqMan® Sample-to-SNP™ Kit Protocol (PN. 4402136).

Bovine Genotypes Panel 2.2 Kit Thermo Scientific™

Thermo Scientific Bovine Genotypes Panel 2.2 Kit is offered for cattle parentage testing and identification using Short Tandem Repeat (STR) DNA loci.

The Bovine Genotypes Panel 2.2 allows co-amplification of the 6 microsatellites in a single multiplex PCR reaction. One primer from each primer pair is end-labeled with a fluorescent dye. After PCR, the fragments are separated and detected in a single electrophoresis injection, using an automated electrophoresis instrument (see Figure 2). In addition, the kit includes bovine control DNA for verification of acceptable PCR and electrophoresis conditions. Reagents and protocols of the Bovine Genotypes Panel 2.2 have been optimized to deliver similar peak sizes (amplification yields) for each loci. Allele callings obtained with this kit represent the true alleles of an individual, instead of 'plus-A peaks' or 'split peaks' typically interpreted when using e.g. a conventional Taq DNA polymerase. The kit employs Phusion Hot Start DNA Polymerase that has a proofreading activity and eliminates the 'plus-A peaks'.

Thermo Scientific Bovine Genotypes Panel 2.2 encompasses the following six STR loci: SPS113, RM067, CSRM60, MGTG4B, CSSM66, and ILSTS006. These are among the list of loci recommended by the International Society for Animal Genetics (ISAG) and the Food and Agriculture Organization of the United Nations (FAO) for genetic studies of domestic animals.

The Bovine Genotypes Panel 2.2 is intended to be used as a complement to the Bovine Genotypes Panel 1.2 when more STR loci are required for resolving a parentage case. The microsatellite alleles of the Bovine Genotypes Panel 2.2 kit can be separated and detected in the same single electrophoresis injection with the microsatellite alleles of the Bovine Genotypes Panel 1.2 kit (see Figure 2).

Highlights

• Various kit choices that include STR loci recommended by ISAG
• Single multiplex reaction - less pipetting steps
• Optimized reagents
• Control DNA provided for verification of PCR and electrophoresis conditions
• Allele calling represents true alleles (no plus-A peaks)

This product has been developed in collaboration with Maxxam Analytics Inc.

StockMarks™ for Horses 17-Plex Genotyping Kit, equine Applied Biosystems™

This PCR-based genotyping system helps validate the parentage of horses quickly and accurately. The StockMarks® for Horses Equine 17-plex Genotyping Kit includes 17 microsatellite markers recommended by the International Society for Animal Genetics (ISAG), co-amplified in a single tube. Protocol sold separately (Cat. No. 4327973).

• PCR process provides greater accuracy than serological methods.
• ISAG-recommended, standardized loci ensure accurate testing.
• Proprietary primer purification process ensures optimal data quality.

Accurate with All Sample Types
All StockMarks® kits amplify microsatellite markers by means of PCR and fluorescent dye-labeled primers. PCR is more accurate than serological typing and requires less sample, is easier to standardize and automate, and is successful with all types of samples, including blood, semen, and hair. Additionally, when fluorescent dye is used to label PCR primers, multiple amplifications can be performed in a single reaction.

Microsatellite-Based Kits Provide Accurate Parentage Testing
The StockMarks® Horse Genotyping System comprises the StockMarks® for Horses Equine Genotyping Kit. This kit both confirm or exclude parentage, using the highly accurate and informative polymorphic short tandem repeats (STRs), also known as microsatellites, which are evenly distributed across the genome.

For Research Use Only. Not for use in diagnostics procedures.

CLA IdentiFiler™ Plus PCR Amplification Kit Applied Biosystems™

The CLA (Cell Line Authentication) IdentiFiler Plus PCR Amplification Kit simplifies the amplification step during authentication of cell lines. It includes the same primers and allelic ladder as the widely used AmpFLSTR Identifiler Plus kit and harnesses next-generation PCR amplification technology to help provide enhanced sensitivity, a cleaner baseline, improved performance with mixtures, and the ability to overcome high levels of PCR inhibition. The kit includes GeneScan 600 LIZ Size Standard v2.0, a five-dye-labeled, high-density size standard for the reproducible sizing of fragment analysis data.

Features of the CLA IdentiFiler Plus PCR Amplification Kit include:
• 5-dye, 16-locus STR kit compatible with all cell line databasing loci standards
• Improved version of legacy AmpFLSTR Identifiler Plus kit
• Size standard included for ease of purchase
• Compatible with SeqStudio, 3730/xL, and 3500/xL genetic analyzers

Cell line authentication using short tandem repeat (STR) analysis kits can be used both for human sample authentication (HSA) and mixed sample analysis (MSA). In HSA, there is a need to verify the DNA profile of the sample to check for sample cross-contamination or sample mix-up, or to perform a quality check. For MSA there is a need to deconvolute the presence of multiple contributors in a sample (chimerism) through identification of multiple DNA genotypes in the sample.

Canine Genotypes Panel 1.1 Kit Thermo Scientific™

Thermo Scientific Canine Genotypes Panel 1.1 is a reagents kit for canine parentage testings and identification using Short Tandem Repeat (STR) DNA loci. Due to their high level of informativeness and Mendelian inheritance, microsatellites have become the markers of choice for parentage testing, identification and forensics.

The Canine Genotypes Panel 1.1 provides all the necessary reagents for amplification of the 19 microsatellite loci. The kit contains master mix and primer mix allows co-amplification of the microsatellites in a single multiplex PCR reaction. Canine control DNA is provided to verify acceptable PCR and electrophoresis conditions. Reagents and protocols of the Canine Genotypes Panel 1.1 kit have been optimized to deliver similar peak sizes (amplification yields) for each loci. Allele callings obtained with this kit represent the true alleles of an individual, instead of 'plus-A peaks' or 'split peaks' typically interpreted when using e.g. a conventional Taq DNA polymerase. The kit employs Phusion Hot Start DNA Polymerase that has a proofreading activity and eliminates the 'plus-A peaks'. One primer from each primer pair is end-labelled with a fluorescent dye. After PCR, the fragments are separated and detected in a single electrophoresis injection, using an automated capillary electrophoresis instrument.

Canine Genotypes Panel 1.1 encompasses the following 19 loci: AHTk211, CXX279, REN169O18, INU055, REN54P11, INRA21, AHT137, REN169D01, AHTh260, AHTk253, INU005, INU030, FH2848, AHT121, FH2054, REN162C04, AHTh171, REN247M23 and Amelogenin (sex determining locus). These markers are included in the 'core panel' of loci recommended by the Applied Genetics Committee of Companying Animals of the International Society for Animal Genetics (ISAG).

Highlights

• Single multiplex PCR of 19 STR loci
• Master mix and Primer mix provided for easy pipetting
• Control DNA provided for verification of PCR and electrophoresis conditions
• Allele calls represents true alleles (no plus-A peaks)

TaqMan™ Sample-to-SNP™ Kit (5 mL sample prep, 1 mL PCR) Applied Biosystems™

The Applied Biosystems® TaqMan® Sample-to-SNP™ Kit allows you to go from Biological Sample to Genotyping Results in less than one hour. The TaqMan® Sample-to-SNP™ Kit combines DNA extraction and TaqMan® chemistry-based genotyping in a streamlined protocol to provide results from any sample in less than one hour.

• DNA Extract All Reagents kits provide PCR-ready DNA from a wide variety of sample types ranging from blood to buccal swabs to plant tissues in 5 min
• The TaqMan®™ GTXpress™ Master Mix provides robust PCR amplification of extracted DNA in less than 50 min
• Compatible with both Fast and Standard mode thermal cycling conditions
• Pre-mixed components stored at 2°–8°C significantly reduce assay setup time
• Validated with all types of Applied Biosystems® TaqMan® Genotyping Assays
• Extended PCR cycling for poorly amplified or sample-limited reactions

The kit includes reagents sufficient for 100 sample preparation reactions at 50 µL/ reaction and 400 PCR reactions at 5 µL for each reaction.

Please note:
The DNA Extract All reagents are not available for individual sale. They are only available as a part of the TaqMan® Sample-to-SNP™ Kit.

MeltDoctor™ HRM Master Mix Applied Biosystems™

Applied Biosystems® MeltDoctor™ HRM Master Mix contains all components (excluding template and primers) formulated for superior HRM performance across a wide range of genomic targets. High Resolution Melting (HRM) analysis is a post-PCR analysis method used to identify genetic variation in nucleic acid sequences. Unlike some competitive mixes, the MeltDoctor™ HRM Master Mix does not require additional mixing prior to use, and was developed and optimized solely for HRM applications.
• Low background fluorescence
• High brightness in the presence of double-stranded DNA
• Minimal temperature shift of DNA melting due to dye binding
• Thermal stability to tolerate PCR cycling conditions
• No inhibition of polymerase activity, resulting in high PCR efficiency
• A dNTP blend including dUTP, which minimizes carryover contamination by allowing amplicon degradation by uracil DNA glycosylase (UDG) in subsequent PCR reactions

Perform Mutation Scanning
HRM analysis can be used to scan for mutations in target genes for the identification of variant samples prior to sequencing analysis. As a mutation scanning technique, HRM offers significant advantages over conventional methods such as Denaturing High- Performance Liquid Chromatography (DHPLC) and Denaturing Gradient Gel Electrophoresis (DGGE). Specifically, the advantages of HRM for mutation scanning include:
• Low reagent consumption, with little waste: HRM requires only a 20 µL PCR reaction for analysis of each sample, eliminating the need for HPLC solvents or DGGE gels
• Simple, fast workflow: no additional instrumentation is required after PCR amplification. A high-resolution melt step can be simply added to the end of the PCR profile for immediate analysis
• Fast optimization: unlike DHPLC, thermal optimization is not required
• Low sample consumption: following HRM analysis, the PCR product can be used directly in a Sanger sequencing reaction

Quality Components
• Magnesium salts and other buffer components, precisely formulated to obtain optimal HRM results.
• AmpliTaq Gold® 360 DNA Polymerase, a highly purified DNA polymerase that provides hot-start performance, minimizing non-specific product formation and enabling reactions to be set up at room temperature
• MeltDoctor™ HRM Dye, a stabilized form of the fluorescent SYTO® 9 double-stranded nucleic acid stain developed by Molecular Probes.

Canine ISAG STR Parentage Kit (2014) Applied Biosystems™

The Canine ISAG STR Parentage Kit (2014) is an optimized reagent kit for the analysis of the 22 short tandem repeat (STR) loci recommended by the International Society of Animal Genetics (ISAG) in 2014 for canine parentage determination.

Key features include:
• Single multiplex PCR reaction optimized for efficient processing of 22 STR loci simultaneously
• Complete kit containing master mix, primer mix, and control DNA for simple analysis
• Incorporation of robust proofreading Phusion DNA polymerase for easy allele calling (no plus-A peaks)

Microsatellites, also referred to as short tandem repeats (STRs), are commonly used for parentage testing, identification, and forensics due to their informativeness and Mendelian inheritance,. The Canine ISAG STR Parentage Kit (2014) provides all of the necessary reagents for simultaneous amplification of the 22 ISAG-recommended microsatellite loci in a single multiplex PCR reaction. Amplicons are sized using capillary electrophoresis on Applied Biosystems genetic analyzers with GeneMapper software to determine each animal’s unique genetic profile. Canine control DNA is provided to verify acceptable PCR and electrophoresis conditions.

The kit reagents and protocols have been optimized to deliver similar peak heights (amplification yields) for each loci and sufficient size and/or fluorescent separation between markers to ensure accurate marker discrimination (Figure 1). Furthermore, allele callings obtained with this kit represent the true alleles of an individual, instead of the 'plus-A peaks' or 'split peaks' typically interpreted when using a conventional TaqDNA polymerase, due to the incorporation of the Phusion Hot Start DNA proofreading polymerase. The performance of the Canine ISAG STR Parentage Kit (2014) has been demonstrated across a wide range of canine breeds, multiple different sample preparation methods, and across different genetic analyzer capillary electrophoresis instruments.

The Canine ISAG STR Parentage Kit (2014) encompasses the following 22 loci: AHTk211, CXX279, REN169O18, INU055, REN54P11, INRA21, AHT137, REN169D01, AHTh260, AHTk253, INU005, INU030, FH2848, AHT121, FH2054, REN162C04, AHTh171, REN247M23. AHTh130, REN105L03, REN64E19, and Amelogenin (sex determining locus).

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