Shop All Magnetic Beads for Human Cell Isolation

Dynabeads™ Untouched™ Human Monocytes Kit (Invitrogen™)

The Dynabeads® Untouched™ Human Monocytes Kit is used to isolate pure and viable monocytes from PBMC by negative isolation. The kit depletes T cells, B cells, NK cells, dendritic cells, granulocytes, and erythrocytes, while the negatively isolated human monocytes are left in the sample. The isolated monocytes cells are in perfect shape for any functional assay and application. Advantages of the Dynabeads® Untouched™ Human Monocytes Kit:

• High purity, recovery, and viability of human monocytes
• Easy to use and to scale up
• No columns required
Magnetic bead-based separation offers easy handling
An antibody mix towards the non-monocytes is added to the sample and allowed to bind to the cells. After a quick wash, the Dynabeads® are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated human monocytes can be directly analyzed in a flow cytometer and used in any application.

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MagniSort™ Human Hematopoietic Lineage Depletion Kit (Invitrogen™)

The MagniSort™ Human Hematopoietic Lineage Depletion Kit is designed for the magnetic depletion of lineage positive cells from normal human peripheral blood mononuclear cells. The kit utilizes a biotinylated antibody cocktail and streptavidin-coated magnetic beads. Undesired cells, including T cells, B cells, NK cells, myeloid cells, granulocytes, and red blood cells, are bound by antibody and then magnetic beads that, when placed in a magnetic field, leave lineage negative cells untouched and free in solution.

The MagniSort™ Human Hematopoietic Lineage Antibody Cocktail contains the following antibodies:
Anti-Human CD2 Biotin
Anti-Human CD3 Biotin
Anti-Human CD10 Biotin
Anti-Human CD11b Biotin
Anti-Human CD14 Biotin
Anti-Human CD16 Biotin
Anti-Human CD19 Biotin
Anti-Human CD56 Biotin
Anti-Human CD123 Biotin
Anti-Human CD235a Biotin

Reported Application
Magnetic Cell Separation

MagniSort™ Human pan-Monocyte Enrichment Kit (Invitrogen™)

The MagniSort™ Human Pan-Monocyte Enrichment Kit is designed for the magnetic separation of conventional CD14+ and unconventional CD16+CD14- monocytes by negative selection. It has been optimized for the isolation of human pan-monocytes from normal human peripheral blood mononuclear cells utilizing a biotinylated antibody cocktail and streptavidin-coated magnetic beads. Undesired cells, including CD16+ NK cells, are bound by antibody and then magnetic beads that, when placed in a magnetic field, leaving monocytes untouched and free in solution.

The MagniSort™ Human Pan-Monocyte Enrichment Antibody Cocktail contains the following antibodies:
Anti-Human CD2 Biotin
Anti-Human CD3 Biotin
Anti-Human CD7 Biotin
Anti-Human CD19 Biotin
Anti-Human CD20 Biotin
Anti-Human CD56 Biotin
Anti-Human CD57 Biotin
Anti-Human CD123 Biotin
Anti-Human CD235a Biotin
Human Fc Receptor Binding Inhibitor Purified


Reported Application
Magnetic Cell Separation

Dynabeads™ FlowComp™ Human CD8 Kit (Invitrogen™)

This gentle and easy-to-use kit allows you to isolate flow-compatible human CD8+ T-cells from PBMC or directly from whole blood. Immediately after positive isolation, the beads are released and removed from your cell sample. In this way, you avoid exposing your cells to potentially cytotoxic or immunogenic foreign substances like iron oxides or dextrans, a potential problem when working with biodegradable particles. In addition, your cells will not be exposed to the stress of being passed through a column. You can expect a purity level of >95% human CD8+ cells.

Advantages:

• No risk of your results being affected by the isolation method
• Gentle tube-based method - no columns needed
• High purity and recovery
• Bead-free cells - compatible with flow cytometry analysis
• Compatible with flow cytometry analysis
• Viable, bead-free CD8+ T-cells for direct use in any cell-based assay.

The isolated cells have the presumed profile of memory and naive CD8+ T cells, and show a high proliferation capacity. You will save time and money, and get increased confidence in your results.

Starting sample:
MNC or whole blood.

Capacity:
The kit will process 2 × 109 mononuclear cells (MNC) or 80 ml whole blood

Dynabeads™ Untouched™ Human B Cells Kit (Invitrogen™)

Use this kit to isolate pure and viable untouched B cells from PBMC by negative isolation. The kit depletes T cells, NK cells, monocytes, platelets, dendritic cells, granulocytes and erythrocytes. The negatively isolated human B cells are left in the sample and have not been in contact with the Dynabeads. The bead and antibody-free B cells are truly untouched, and in perfect shape for any functional assay and application.

• High purity, recovery and viability of human untouched B cells
• Easy to use and to scale up
• No columns required

An antibody mix towards the non-B cells is added to the sample and allowed to bind to the cells. Dynabeads® are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched human B cells can be directly analyzed in a flow cytometer and used in any application.

Starting Sample:
PBMC

Dynabeads™ CD8 (Invitrogen™)

Dynabeads® CD8 are superparamagnetic beads coupled with an anti-human CD8 antibody that enable easy isolation or depletion of human CD8+ T cells directly from any sample, including whole blood, bone marrow, buffy coat, mononuclear cells (MNC), and tissue digests. Advantages of Dynabeads® CD8:

• Fast and efficient isolation of CD8+ T cells from any sample—no columns required
• High purity, yield, and viability of positively isolated CD8+ T cells for molecular downstream assays
• Fast and efficient depletion of CD8+ T cells from any sample

About Dynabeads® CD8
Dynabeads® CD8 are uniform, superparamagnetic beads (4.5 µm diameter) coated with a primary monoclonal antibody specific for the CD8 membrane antigen mainly expressed on human cytotoxic T cells. Because of the bead size, Dynabeads® CD8 can easily and efficiently isolate or deplete cells from viscous samples such as whole blood and bone marrow in about 30 minutes. Dynabeads® CD8 will also afford excellent recovery of high-purity, viable cells for use in downstream molecular studies; for example, those in which cells are to be lysed while still attached to beads, and nucleic acids or proteins further purified. Note that intact cells will not be released from these beads, so if you want to isolate CD8+ cells for cell-based applications, or you need to check your samples with flow cytometry, check out our total human CD8 product offerings.

Magnetic bead–based separation offers easy handling
Dynabeads® CD8 is added to the sample under continuous mixing to optimize the binding of the Dynabeads® to the target cells. By placing the sample on a magnet, it separates the bead-bound target cells from the rest of the sample in just 1–2 minutes. For depletion, remove the supernatant to a new tube for further studies and discard the bead-bound cells. For positive isolation for molecular studies, remove the supernatant and wash the bead-bound cells 2–3 times in buffer to get optimal purity. The cells can be lysed while still attached to the beads and the supernatant transferred to a new tube for downstream molecular analysis. Starting samples can be whole blood, buffy coat, bone marrow, PBMC, or tissue digests.

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Dynabeads™ Untouched™ Human CD8 T Cells Kit (Invitrogen™)

The Dynabeads® Untouched™ Human CD8 T Cells Kit is used to isolate pure and viable untouched CD8+ T cells from PBMC by negative isolation. The kit depletes CD4+ T cells, B cells, NK cells, monocytes, platelets, dendritic cells, granulocytes, and erythrocytes. The negatively isolated human CD8+ T-cells are left in the sample and have not been in contact with the Dynabeads®. The bead- and antibody-free CD8+ T cells are truly untouched, and in perfect shape for any functional assay or application.

Advantages of the Dynabeads® Untouched™ Human CD8 T Cells Kit:

• High purity, recovery, and viability of human untouched CD8+ T cells
• Easy to use and to scale up
• No columns required

Magnetic bead-based Separation offers easy handling
An antibody mix towards the non-CD8 cells is added to the sample and allowed to bind to the cells. Dynabeads® are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched human CD8+ T cells can be directly analyzed in a flow cytometer and used in any application. For depletion or positive isolation of CD8+ cells, select from our range of Human CD8+ T Cell Isolation Products.

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Dynabeads™ CD4 (Invitrogen™)

Dynabeads® CD4 are superparamagnetic beads coupled with an anti-human CD4 antibody that enable easy isolation or deplettion of human CD4+ T cells directly from any sample, including whole blood, bone marrow, buffy coat, mononuclear cells (MNC), and tissue digests. Advantages of Dynabeads® CD4:

• Fast and efficient isolation of CD4+ T cells from any sample—no columns required
• High purity, yield, and viability of positively isolated CD4+ T cells for molecular downstream assays
• Fast and efficient depletion of CD4+ T cells from any sample

About Dynabeads® CD4
Dynabeads® CD4 are uniform, superparamagnetic beads (4.5 µm diameter) coated with a primary monoclonal antibody specific for the CD4 membrane antigen mainly expressed on human T helper cells. Because of the bead size, Dynabeads® CD4 can easily and efficiently isolate or deplete cells from viscous samples such as whole blood and bone marrow in about 30 minutes. Dynabeads® CD4 will also afford excellent recovery of high-purity, viable cells for use in downstream molecular studies; for example, those in which cells are to be lysed while still attached to beads, and nucleic acids or proteins further purified. Note that intact cells will not be released from these beads, so if you want to isolate CD4+ cells for cell-based applications, or you need to check your samples with flow cytometry, check out our total human CD4 product offerings.

Magnetic bead–based separation offers easy handling
Dynabeads® CD4 is added to the sample under continuous mixing to optimize the binding of the Dynabeads® to the target cells. By placing the sample on a magnet, it separates the bead-bound target cells from the rest of the sample in just 1–2 minutes. For depletion, remove the supernatant to a new tube for further studies and discard the bead-bound cells. For positive isolation for molecular studies, remove the supernatant and wash the bead-bound cells 2–3 times in buffer to get optimal purity. The cells can be lysed while still attached to the beads and the supernatant transferred to a new tube for downstream molecular analysis. Starting samples can be whole blood, buffy coat, bone marrow, PBMC, or tissue digests.

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Dynabeads™ CD31 Endothelial Cell (Invitrogen™)

Isolate endothelial cells directly from tissue digests. Dynabeads® CD31 are ideal for positively isolating endothelial cells for downstream molecular analysis.

• Positively isolate endothelial cells directly from tissue digests for molecular applications
• Establish cultures for endothelial cells
• No columns required

Starting sample:
Tissue digests.

MagniSort™ Human B Cell Enrichment Kit (2nd Generation) (Invitrogen™)

The MagniSort™ Human B cell Enrichment Kit (2nd Generation) is designed for the magnetic separation of B cells by negative selection with improved purity. It has been optimized for the isolation of human B cells from normal human peripheral blood mononuclear cells utilizing a biotinylated antibody cocktail and streptavidin-coated magnetic beads. Undesired cells are bound by antibody and then magnetic beads that, when placed in a magnetic field, leave B cells untouched and free in solution.

The MagniSort™ Human B cell Enrichment Antibody Cocktail (2nd Generation) contains the following antibodies:
Anti-Human CD2 Biotin
Anti-Human CD3 Biotin
Anti-Human CD11b Biotin
Anti-Human CD14 Biotin
Anti-Human CD16 Biotin
Anti-Human CD36 Biotin
Anti-Human CD56 Biotin
Anti-Human CD235a Biotin

Reported Application
Magnetic Cell Separation

Dynabeads™ Regulatory CD4+/CD25+ T Cell Kit (Invitrogen™)

Use this kit to isolate highly pure CD4+ CD25+ regulatory T-cells (Treg) that express the intracellular transcription factor Foxp3. The CD4+ CD25- cell fraction can be used as effector cells in downstream inhibitory assays. You can expect >95% purity (CD4+ CD25+ expression) and >80% Foxp3 expression.

Isolate bead- and antibody-free human CD4+ CD25+ Foxp3+ Treg cells

Treg cells can be expanded with Dynabeads® CD3/CD28 Treg Expander and remain
Foxp3+.

Treg cells can be used in any downstream applications (e.g., suppression assays)

Methodology

The isolation procedure includes the following steps:

• Negative isolation of untouched human CD4+ T-cells from PBMC.
• Positive isolation of CD25+ T-cells from the untouched CD4+ T-cell subset.
• Removal of beads from the CD4+CD25+ Treg cells with DETACHaBEAD®.

Starting sample: PBMC

Dynabeads™ Untouched™ Human CD4 T Cells Kit (Invitrogen™)

Use this kit to isolate pure and viable untouched CD4+ T-cells from PBMC by negative isolation. The kit depletes CD8+ T cells, B cells, NK cells, monocytes, platelets, dendritic cells, granulocytes and erythrocytes. The negatively isolated human CD4+ T-cells are left in the sample and have not been in contact with the Dynabeads. The bead and antibody-free CD4+ T-cells are truly untouched, and in perfect shape for any functional assay and application.

• High purity, recovery and viability of human untouched CD4+ T cells
• Easy to use and to scale up
• No columns required

Methodology: An antibody mix towards the non-CD4 cells is added to the sample and allowed to bind to the cells. Dynabeads® are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched human CD4+ T-cells can be directly analyzed in a flow cytometer and used in any application.

Note: A negative isolation strategy isolates pure and truly untouched cells. When maximum cell purity is critical, a positive cell isolation strategy is recommended

Starting Sample:
PBMC

Dynabeads™ CD15 (Invitrogen™)

Dynabeads® CD15 are superparamagnetic beads covalently coupled with an anti-human CD15 antibody that efficiently depletes human CD15+ myeloid cells (predominantly neutrophil and eosinophil granulocytes) directly from whole blood, buffy coat, or PBMC samples. Dynabeads® CD15 can also be used to positively isolate CD15+ cells for molecular applications. Advantages of Dynabeads® CD15:

• Efficient depletion of granulocytes (neutrophils and eosinophils)
• Isolation of granulocytes directly from whole blood for molecular applications
• No columns required

About Dynabeads® CD15
Dynabeads® CD15 are uniform, superparamagnetic polystyrene beads (4.5 µm diameter) coated with a monoclonal mouse IgM antibody recognizing the human CD15 membrane antigen. The CD15 antigen is predominantly expressed on human neutrophil and eosinophil granulocytes, and to a varying degree on monocytes.

Magnetic bead-;based separation offers easy handling
Dynabeads® CD15 is added to the sample under continuous mixing to optimize the binding of the Dynabeads® to the target cells. By placing the sample on a magnet, it separates the bead-bound target cells from the rest of the sample in just 1–2 minutes. For depletion, remove the supernatant to a new tube for further studies and discard the bead-bound cells. For positive isolation for molecular studies, remove the supernatant and wash the bead-bound cells 2–3 times in buffer to get optimal purity. The cells can be lysed while still attached to the beads and the supernatant transferred to a new tube for downstream molecular analysis. Starting samples can be whole blood, PBMC, or buffy coat.

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MagniSort™ Human CD45 Depletion Kit (Invitrogen™)

The MagniSort™ Human CD45 Depletion Kit is designed to deplete CD45+ cells from human lysed whole blood or peripheral blood mononuclear cells, utilizing a biotinylated Anti-Human CD45 antibody and streptavidin-coated magnetic beads. CD45+ cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the CD45- cells can be separated from CD45+ cells by decanting.

After depletion, the purity of selected cells can be verified by staining with Anti-Human CD45, clone 2D1.

Reported Application
Magnetic Cell Separation

CELLection™ Epithelial Enrich Dynabeads™ (Invitrogen™)

Use this kit to obtain up to 5-log enrichment of human epithelial tumor cells, directly from whole blood, bone marrow or PBMC. Tumor cell clumps can be enriched from bone marrow. Enriched cells are bead-free, viable and suitable for immunocytochemical staining or any other downstream application.

High enrichment of viable human tumor cells

Isolate directly from whole blood, bone marrow or PBMC
Viable cells for any cellular applications

Methodology: Add CELLection™ Dynabeads® to the sample. The beads will bind the tumor cells after a short incubation. The bead-bound tumor cells are quickly separated on a magnet and subsequently released from the beads with the supplied Release Buffer. (CELLection™ Dynabeads® contains a DNA linker that is cleaved by the DNase I in the Release Buffer.)

Note: If you want to deplete or use the isolated tumor cells for molecular applications, please use the equivalent product Dynabeads® Epithelial Enrich (Cat. No. 161-02). This product contains the same antibody, but with no release of the cells from the beads after positive isolation.

Starting Samples: Whole blood, bone marrow, buffy coat, PBMC.