Shop All Magnetic Beads for Mouse Cell Isolation

MagniSort™ Mouse CD335 (NKp46) Positive Selection Kit Invitrogen™

The MagniSort™ Mouse CD335 (NKp46) Positive Selection Kit is designed for the magnetic separation of CD335+ cells by positive selection. It has been optimized for the isolation of CD335+ cells from mouse spleens or lymph nodes utilizing a biotinylated Anti-Mouse CD335+ antibody and streptavidin-coated magnetic beads. CD335+ cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the undesired cells can be separated from CD335+ cells by decanting.

After positive selection, the purity of selected cells can be verified by staining with Anti-Mouse CD49b (Integrin alpha 2), clone DX5, or Anti-Mouse NK1.1, clone PK136, in C57BL, FVB/N and NZB mice.

Reported Application
Magnetic Cell Separation

Dynabeads™ Mouse Pan T (Thy1.2) Invitrogen™

Dynabeads® Mouse pan T (Thy 1.2) are magnetic beads that provide a robust and versatile tool to isolate or deplete mouse T-cells directly from any sample, including tissue digests such as spleen or lymph nodes, whole blood, bone marrow, and mononuclear cells (MNC).
• Rapid isolation of mouse T-cells from any sample – no columns required
• Positive isolation for molecular downstream assays or efficient depletion of mouse T cells

Excellent Yield, Purity, and Viability of Recovered Cells
Dynabeads® Mouse pan T (Thy 1.2) are uniform, superparamagnetic beads (4.5 µm diameter) that enable easy isolation of mouse T-cells from spleen or lymph nodes, although any single-cell suspension may be used. Dynabeads® Mouse pan T beads are coated with a primary monoclonal antibody specific for the Thy 1.2 membrane antigen expressed on peripheral T cells, thymocytes and intraepithelial T lymphocytes of all common mouse strains. Dynabeads® Mouse pan T binds to T-cells during a short incubation period in a tube, after which the bead-bound cells are separated from unbound cells using a magnet. This fast and gentle isolation method does not require the use of columns, and helps ensure high purity, recovery and viability of the isolated T cells.

Ideal for Both Depletion and Positive Isolation for Downstream Molecular Assays
Because of the powerful magnets used for separation, Dynabeads® Mouse pan T (Thy 1.2) can be used in viscous samples such as single cell suspensions from tissue, whole blood and bone marrow, and enable efficient depletion of mouse T-cells in about 30 minutes. Dynabeads® Mouse pan T will also afford excellent recovery of high-purity, viable cells when used for positive isolation for downstream studies; for example, those in which cells are to be lysed while still attached to beads, and nucleic acids or proteins further purified. Note intact cells will not be released from these beads, so if you want to isolate mouse T-cells for cell-based applications, or you need to check your samples with flow cytometry, you should use Dynabeads® FlowComp Mouse Pan T (CD90.2) (for bead-free cells).

Select what is right for you from our range of Mouse T-Cell Isolation Products.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

MagniSort™ Mouse CD49b Positive Selection Kit Invitrogen™

The MagniSort™ Mouse CD49b Positive Selection Kit is designed for the magnetic separation of CD49b+ cells by positive selection. It has been optimized for the isolation of CD49b+ cells from mouse spleens or lymph nodes utilizing a biotinylated Anti-Mouse CD49b antibody and streptavidin-coated magnetic beads. CD49b+ cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the undesired cells can be separated from CD49b+ cells by decanting.

After positive selection, the purity of selected cells can be verified by staining with Anti-Mouse CD49b (integrin alpha 2), clone DX5, and Anti-Mouse NK1.1, clone PK136. For mouse strains that do not express NK1.1 antigen such as BALB/c, SJL, AKR, CBA C3H, Anti-Mouse CD335 (NKp46), clone 29A1.4, may be used.

Reported Application
Magnetic Cell Separation

Dynabeads™ Mouse DC (Dendritic Cell) Enrichment Invitrogen™

Use this kit to enrich viable mouse dendritic cells (DC's) from spleen/lymph node cells by negative isolation. The kit depletes T cells, mIgM+ B cells, NK cells, erythrocytes and most granulocytes. Highly enriched mouse DC's are bead and antibody-free, and can be used in any functional assay. The cells can be further sorted to obtain high purity of specific DC sub-populations.

Obtain high recovery (80-100%) of viable Lin-CD11c+ DC's of all sup-populations, including DC's positive for CD4, CD8α, B220 and CD19.

• Substantial reduction in sorting time and volume compared to sorting only, thus saving money in addition.
• Pool cells/tissue for easy sample scale -up

Methodology:
An antibody mix towards the non-dendritic cells is added to the sample and allowed to bind to the cells. Dynabeads® are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining untouched mouse DC's can be directly sorted into the sup-population of choice or used in any other application.

Starting samples:
Spleen or lymph node cells.

MagniSort™ Mouse CD3 Positive Selection Kit Invitrogen™

The MagniSort™ Mouse CD3 Positive Selection Kit is designed for the magnetic separation of CD3+ cells by positive selection. It has been optimized for the isolation of CD3+ cells from mouse spleens or lymph nodes utilizing a biotinylated Anti-Mouse CD3 antibody and streptavidin-coated magnetic beads. CD3+ cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the undesired cells can be separated from CD3+ cells by decanting.

After positive selection, the purity of selected cells can be verified by staining with Anti-Mouse CD3e, clone 145-2C11.

Reported Application
Magnetic Cell Separation

Dynabeads™ Mouse CD8 (Lyt 2) Invitrogen™

Dynabeads® Mouse CD8 (Lyt 2) are magnetic beads that provide a robust and versatile tool to isolate or deplete mouse CD8+ T-cells directly from any sample, including tissue digests such as spleen or lymph nodes, whole blood, bone marrow, and mononuclear cells (MNC).
• Rapid isolation of mouse CD8+ T-cells from any sample – no columns required
• Positive isolation for molecular downstream assays or efficient depletion of mouse CD8+ T cells

Excellent Yield, Purity, and Viability of Recovered Cells
Dynabeads® Mouse CD8 (Lyt 2) are uniform, superparamagnetic beads (4.5 µm diameter) that enable easy isolation of mouse CD8+ cells from spleen or lymph nodes, although any single-cell suspension may be used. Dynabeads® Mouse CD8 beads are coated with a primary monoclonal antibody specific for the Lyt 2 membrane antigen mainly expressed on cytotoxic/suppressor subpopulation of mature T-cells of all common mouse strains. Dynabeads® Mouse CD8 binds to CD8+ cells during a short incubation period in a tube, after which the bead-bound cells are separated from unbound cells using a magnet. This fast and gentle isolation method does not require the use of columns, and helps ensure high purity, recovery and viability of the isolated CD8+ cells.

Ideal for Both Depletion and Positive Isolation for Downstream Molecular Assays
Because of the powerful magnets used for separation, Dynabeads® Mouse CD8 (Lyt 2) can be used in viscous samples such as single cell suspensions from tissue, whole blood and bone marrow, and enable efficient depletion of mouse CD8+ cells in about 30 minutes. Dynabeads® Mouse CD8 (Lyt 2) will also afford excellent recovery of high-purity, viable cells when used for positive isolation for downstream studies; for example, those in which cells are to be lysed while still attached to beads, and nucleic acids or proteins further purified. Note that intact cells will not be released from these beads, so if you want to isolate mouse CD8+ cells for cell-based applications, or you need to check your samples with flow cytometry, you should use Dynabeads® FlowComp Mouse CD8 Cells (for bead-free cells).

Select what is right for you from our range of Mouse CD8 Isolation Products.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

MagniSort™ Mouse CD45.1 Positive Selection Kit Invitrogen™

The MagniSort™ Mouse CD45.1 Positive Selection Kit is designed for the magnetic separation of CD45.1+ cells by positive selection. It has been optimized for the isolation of CD45.1+ cells from mouse spleens or lymph nodes utilizing a biotinylated Anti-Mouse CD45.1 antibody and streptavidin-coated magnetic beads. CD45.1+ cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the undesired cells can be separated from CD45.1+ cells by decanting.

After positive selection, the purity of selected cells can be verified by staining with Anti-Mouse CD45.1, clone A20.

Reported Application
Magnetic Cell Separation

MagniSort™ Mouse CD90.1 Positive Selection Kit Invitrogen™

The MagniSort™ Mouse CD90.1 Positive Selection Kit is designed for the magnetic separation of CD90.1+ cells by positive selection. It has been optimized for the isolation of CD90.1+ cells from mouse spleens or lymph nodes utilizing a biotinylated Anti-Mouse CD90.1 antibody and streptavidin-coated magnetic beads. CD90.1+ cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the undesired cells can be separated from CD90.1+ cells by decanting.

After positive selection, the purity of selected cells can be verified by staining with Anti-Mouse/Rat CD90.1, clone HIS51.

Reported Application
Magnetic Cell Separation

MagniSort™ Mouse F4/80 Positive Selection Kit Invitrogen™

The MagniSort™ Mouse F4/80 Positive Selection Kit is designed for the magnetic separation of F4/80+ cells by positive selection. It has been optimized for the isolation of F4/80+ cells from mouse bone marrow or splenocytes utilizing a biotinylated Anti-Mouse F4/80 antibody and streptavidin-coated magnetic beads. F4/80+ cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the undesired cells can be separated from F4/80+ cells by decanting.

After positive selection, the purity of selected cells can be verified by staining with Anti-Mouse F4/80, clone BM8, and Anti-Mouse CD11b, clones M1/70.

Reported Application
Magnetic Cell Separation

Dynabeads™ Mouse Pan B (B220) Invitrogen™

Dynabeads® Mouse pan B (B220) are magnetic beads that provide a robust and versatile tool to isolate or deplete mouse T-cells directly from any sample, including tissue digests such as spleen or lymph nodes, whole blood, bone marrow, and mononuclear cells (MNC).
• Rapid isolation of mouse T-cells from any sample – no columns required
• Positive isolation for molecular downstream assays or efficient depletion of mouse B cells

Excellent Yield, Purity, and Viability of Recovered Cells
Dynabeads® Mouse pan B (B220) are uniform, superparamagnetic beads (4.5 µm diameter) that enable easy isolation of mouse B cells from spleen or lymph nodes, although any single cell suspension may be used. Dynabeads® Mouse pan B beads are coated with a primary monoclonal antibody specific for the B220 membrane antigen. B220 (also known as CD45R) is expressed on all B cells throughout development, from the early pro-B stage of B cell differentiation. Dynabeads® Mouse pan B binds to B cells during a short incubation period in a tube, after which the bead-bound cells are separated from unbound cells using a magnet. This fast and gentle isolation method does not require the use of columns, and helps ensure high purity, recovery and viability of the isolated B cells.

Ideal for Both Depletion and Positive Isolation for Downstream Molecular Assays
Because of the powerful magnets used for separation, Dynabeads® Mouse pan B (B220) can be used in viscous samples such as single cell suspensions from tissue, whole blood and bone marrow, and enable the efficient depletion of mouse B cells in about 30 minutes. Dynabeads® Mouse pan B will also afford excellent recovery of high-purity, viable cells when used for positive isolation for downstream studies; for example, those in which cells are to be lysed while still attached to beads, and nucleic acids or proteins further purified. Note that viable cells will not be released from these beads, so if you want to isolate mouse B cells for cell-based applications, or you need to check your samples with flow cytometry, you should use Dynabeads® Mouse CD43 (Untouched™ B cells).

Select what is right for you from our range of Mouse B cell Isolation Products.

For Research Use Only. Not for human or animal therapeutic or diagnostic use.

MagniSort™ Mouse CD4 Positive Selection Kit Invitrogen™

The MagniSort™ Mouse CD4 Positive Selection Kit is designed for the magnetic separation of CD4+ cells by positive selection. It has been optimized for the isolation of CD4+ cells from mouse spleens or lymph nodes utilizing a biotinylated Anti-Mouse CD4 antibody and streptavidin-coated magnetic beads. CD4+ cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the undesired cells can be separated from CD4+ cells by decanting.

After positive selection, the purity of selected cells can be verified by staining with Anti-Mouse CD4, clones RM4-5 or GK1.5.

Reported Application
Magnetic Cell Separation

MagniSort™ Mouse NK cell Enrichment Kit Invitrogen™

The MagniSort™ Mouse NK cell Enrichment Kit is designed for the magnetic separation of mouse NK cells by negative selection. This kit will not isolate NKT cells. It has been optimized for the isolation of mouse NK cells from spleens utilizing a biotinylated antibody cocktail and streptavidin-coated magnetic beads. Undesired cells are bound by antibody and then magnetic beads that, when placed in a magnetic field, leave NK cells untouched and free in solution.

The MagniSort™ Mouse NK cell Enrichment Antibody Cocktail contains the following antibodies:
Anti-Mouse CD3 Biotin
Anti-Mouse CD4 Biotin
Anti-Mouse CD8 Biotin
Anti-Mouse CD19 Biotin
Anti-Mouse MHC Class II (I-A/I-E) Biotin
Anti-Mouse F4/80 Antigen Biotin
Anti-Mouse Ly-6G (Gr-1) Biotin
Anti-Mouse TER-119 Biotin

Reported Application
Magnetic Cell Separation

MagniSort™ Mouse CD45.2 Positive Selection Kit Invitrogen™

The MagniSort™ Mouse CD45.2 Positive Selection Kit is designed for the magnetic separation of CD45.2+ cells by positive selection. It has been optimized for the isolation of CD45.2+ cells from mouse spleens or lymph nodes utilizing a biotinylated Anti-Mouse CD45.2 antibody and streptavidin-coated magnetic beads. CD45.2+ cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the undesired cells can be separated from CD45.2+ cells by decanting.

After positive selection, the purity of selected cells can be verified by staining with Anti-Mouse CD45.2, clone 104.

Reported Application
Magnetic Cell Separation

MagniSort™ Mouse CD90.2 Positive Selection Kit Invitrogen™

The MagniSort™ Mouse CD90.2 Positive Selection Kit is designed for the magnetic separation of CD90.2+ cells by positive selection. It has been optimized for the isolation of CD90.2+ cells from mouse spleens or lymph nodes utilizing a biotinylated Anti-Mouse CD90.2 antibody and streptavidin-coated magnetic beads. CD90.2+ cells are bound by antibody and then magnetic beads. When placed in a magnetic field, the undesired cells can be separated from CD90.2+ cells by decanting.

After positive selection, the purity of selected cells can be verified by staining with Anti-Mouse CD90.2, clone 53-2.1.

Reported Application
Magnetic Cell Separation

Dynabeads™ FlowComp™ Mouse CD4 Kit Invitrogen™

This gentle and easy-to-use kit allows you to isolate flow-compatible mouse CD4+ T cells. Immediately after positive isolation, the beads are released and removed from your cell sample. In this way, you avoid exposing your cells to potentially cytotoxic or immunogenic foreign substances like iron oxides or dextrans, a potential problem when working with biodegradable particles. In addition, your cells will not be exposed to the stress of being passed through a column.

You can expect a purity level of >85% and viability of >85% of mouse CD4+ cells.

Advantages:

• No risk of your results being affected by the isolation method
• Gentle tube-based method - no columns needed
• High purity, recovery and viability
• Bead-free cells - compatible with flow cytometry analysis
• Viable, bead-free CD4+ T-cells for direct use in any cell-based assay.

The isolated cells have the presumed profile of memory and naive CD4+ T cells, and show a high proliferation capacity. You will save time and money, and get increased confidence in your results.

Starting sample: Spleen or lymph node cells
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