Shop All Sequencing Buffers & Reagents

SOLiD™ Buffer E, Double (Applied Biosystems™)

The SOLiD™ Buffers are needed for ligation sequencing on the 5500⁄5500xl Genetic Analyzer. SOLiD™ Buffer E, Double (PN 4463016) consists of SOLiD™ Buffer E Part 1 and SOLiD™ Buffer E Part 2, which need to be mixed prior to running on the instrument. SOLiD™ Buffer E, Double contains sufficient reagents to run 950 to 1632 cycles, or up to 136 cycles per lane.

These kits are individually orderable components of the SOLiD™ Run Cycle Buffer Kit (PN 4461840). Supplemental buffers can be used to replace components of the main buffer kit. Each double size has sufficient buffer for covering two FlowChips.

5500 W Buffer G (Applied Biosystems™)

5500 W Buffer G is needed for ligation sequencing on the 5500 W Series genetic analyzers. Supplemental buffers like this one can be used to replace components of the main buffer kit. 5500 W Buffer G contains sufficient reagents to run up to 432 cycles, or 72 cycles per lane for 6 lanes.

SOLiD™ Buffer A, Double (Applied Biosystems™)

The SOLiD™ Buffers are needed for ligation sequencing on the 5500⁄5500xl Genetic Analyzer. SOLiD™ Buffer A, Double (PN 4463024) consists of SOLiD™ Buffer A Part 1 and SOLiD™ Buffer A Part 2, which need to be mixed prior to running on the instrument. SOLiD™ Buffer A, Double (PN 4463024) contains sufficient reagents to run 950 to 1632 cycles, or up to 136 cycles per lane.

These kits are individually orderable components of the SOLiD™ Run Cycle Buffer Kit (PN 4461840). Supplemental buffers can be used to replace components of the main buffer kit. Each double size has sufficient buffer for covering two FlowChips.

Solvent A3, 3.5% THF/Water, 960 mL (Applied Biosystems™)

This reagent is intended for N-terminal protein sequencing applications.

Use with other protein sequencing reagents: 20% Acetonitrile in Water, 5% PITC in N-Heptane, 25% Trifluoroacetic Acid in Water, Ethyl Acetate, N-Butyl Chloride, Isopropanol and Acetonitrile

For Research Use Only. Not for use in diagnostics procedures.

TFA HPLC Grade, 10 mL (Applied Biosystems™)

This reagent is intended for N-terminal protein sequencing applications.

For Research Use Only. Not for use in diagnostics procedures.

5500 W Buffer E (Applied Biosystems™)

5500 W Buffer E is needed for ligation sequencing on the 5500 W Series genetic analyzers. Supplemental buffers like this one can be used to replace components of the main buffer kit. 5500 W Buffer E consists of 5500 W Buffer E Part 1 and 5500 W Buffer E Part 2, which need to be mixed prior to running on the instrument. 5500 W Buffer E contains sufficient reagents to run up to 432 cycles, or 72 cycles per lane for 6 lanes.

SequenceRx Enhancer Solution A (Invitrogen™)

SequenceRx Enhancer Solution A is a novel reaction co-solvent that facilitate sequencing through problematic GC-rich regions,direct repeats, inverted repeats, hairpins, and/or short nucleotide repeats. The component is added to the reaction mixture, and the mixture is incubated in a thermocycler as usual. Early terminations and/or low signal strength aretypically improved within the troublesome region, permitting additional sequence data to be obtained. SequenceRx Enhancer Solution A is fully compatible with BigDye terminator, dRhodamine dye terminator, and rhodaminedye terminator chemistries and all sequencing platforms.

R4A (25% Trifluoroacetic Acid in Water) (Applied Biosystems™)

This reagent is intended for N-terminal protein sequencing applications.

Use with other protein sequencing reagents: 20% Acetonitrile in Water, S4B, 200 mL, R1, 5% PITC in N-Heptane, 40 mL, R3, Trifluoroacetic Acid, 40 mL, S3, N-Butyl Chloride, 200 mL, Solvent A3, 3.5% THF⁄Water, 960 mL

For Research Use Only. Not for use in diagnostics procedures.

Solvent B2, Isopropanol & Acetonitrile (Applied Biosystems™)

This reagent is intended for N-terminal protein sequencing applications.

Use with other protein sequencing reagents: 20% Acetonitrile in Water, 5% PITC in N-Heptane, Trifluoroacetic Acid, 25% Trifluoroacetic Acid in Water, Ethyl Acetate, N-Butyl Chloride, 3.5% THF⁄Water

For Research Use Only. Not for use in diagnostics procedures.

Biobrene™ Plus, 75 mg

This reagent is intended for N-terminal protein sequencing applications.

For Research Use Only. Not for use in diagnostics procedures.

Anode Buffer Container (ABC) 3500 Series (Applied Biosystems™)

The anode buffer container (ABC) contains 1x running buffer to support all electrophoresis applications on the Applied Biosystems 3500 series Genetic Analyzers. It is made in a ready-to-use, disposable container with a radio frequency identification (RFID) tag incorporated into the label. The top of the ABC is heat sealed with a plastic film, which should be removed prior to direct installation on to the instrument. Each package includes four individual containers.

For Research Use Only. Not for use in diagnostics procedures.

S4C, 10% Acetonitrile in Water, 200 mL (Applied Biosystems™)

This reagent is intended for N-terminal protein sequencing applications.

For Research Use Only. Not for use in diagnostics procedures.

BigDye™ Terminator v1.0 & v3.0 5X Sequencing Buffer (Applied Biosystems™)

This sequencing buffer is optimized for use with BigDye® Terminator v1.0 or v3.0 cycle sequencing kits.

For Research Use Only. Not for use in diagnostics procedures.

Acetonitrile in Water (20%), S4B (Applied Biosystems™)

This reagent is intended for N-terminal protein sequencing applications.

Use with other protein sequencing reagents: 20% Acetonitrile in Water, S4B, 200 mL, Procise® Sequencing System (Model 492 and 294), 5% PITC in N-Heptane (40 mL), 25% Trifluoroacetic Acid in Water (40 mL), Ethyl Acetate (450 mL), N-Butyl Chloride (200 mL), Solvent A3, 3.5% THF⁄Water (960 mL), Solvent B2, Isopropanol and Acetonitrile (1 L).

For Research Use Only. Not for use in diagnostics procedures.

SOLiD™ Buffer F, Double, v2 (Applied Biosystems™)

SOLiD™ buffers are needed for ligation sequencing on the 5500/5500xl Genetic Analyzer. These buffers are individually orderable components of the SOLiD™ Run Cycle Buffer Kit. This double size buffer has sufficient quantity for two FlowChips.

SOLiD™ Buffer F, Double, v2 contains sufficient reagents to run 950 to 1632 cycles or up to 136 cycles per lane. SOLiD™ Buffer F, Double, v2 comes at a 10X concentration and must be diluted to 1X before use.

For Research Use Only. Not for any animal or human therapeutic or diagnostic use.