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BigDye™ Direct Cycle Sequencing Kit (Applied Biosystems™)

The BigDye® Direct Cycle Sequencing Kit, part of the BigDye® product family, is a complete kit for your PCR and Sequencing needs. This innovative, time-saving kit combines the PCR Purification and Cycle Sequencing Steps, enabling a streamlined protocol and shorter time to results. The kit is available in 24, 100, and 1000-reaction sizes to fit your sequencing throughput needs.

The BigDye® Direct Cycle Sequencing Kit Gives You These Advantages:
• A streamlined, simple procedure
• Performance of all sequencing steps in a single reaction tube
• Fast migration with excellent resolution starting at the 1st base after the gene-specific primer sequence
• Substantial time savings versus previous workflows

Save Time and Get Better Results with a Unique Streamlined Workflow
The BigDye® Direct Cycle Sequencing Kits have been developed to minimize complexity and hands-on time. Compared with previous sequencing procedures, which typically require 5 processing steps and take up to 8 hours, Big Dye® Direct can deliver results in 4 steps taking approximately 5 hours—up to a 40% time savings (Figure 1).

The kit provides reagents for 2 key steps of the sequencing workflow:
• The PCR reaction – using the BigDye® Direct PCR Master Mix and M13 tailed primer (not provided); PCR is performed in approximately 1 hour.
• The unique one-step PCR purification-cycle sequencing reaction – using the BigDye® Direct Sequencing Master Mix and the BigDye® Direct M13 primers provided (M13 tailed primer used for the PCR reaction simplifies the sequencing reaction setup by using 2 sequencing primers independently of the PCR product analyzed).

A One-Tube Protocol Gives You More Confidence
With the BigDye® Direct Cycle Sequencing Kit, all steps from PCR to loading on the capillary can be performed in the same reaction tube without transferring material from one tube to another. By minimizing the number of manual handling steps, the possibility of experimental variability is reduced; therefore, you can have greater confidence in your data.

An Integrated Solution Ideal for Resequencing
The BigDye® Direct Cycle Sequencing Kit is the first product designed to maintain conditions leading to high quality data generation throughout the entire read length with faster run times compared to common sequencing workflows. This is achieved through a unique reagent formulation that allows high-resolution data reads closer to the primer and the use of POP-7™ polymer (Figure 2) or SeqStudio cartridge for faster capillary electrophoresis times. The BigDye® Direct Cycle Sequencing Kit is optimized to work with Applied Biosystems® Genetic Analyzers and the BigDye XTerminator® Purification Kit.

Oncomine™ Breast cfDNA Research Assay v2 (Ion Torrent™)

The Oncomine Breast cfDNA Assay v2 is part of a complete solution to detect breast tumor-derived DNA (ctDNA) in cell-free DNA (cfDNA). It provides the reagents for library construction and a single pool of multiplex PCR primers for preparation of amplicon libraries from cfDNA obtained from the plasma fraction of a single 10 mL tube of whole blood.

Liquid biopsies offer several advantages over conventional solid tumor biopsies:
• Less invasive, enabling them to be taken at multiple time points to monitor progression of the cancer
• Lower cost
• Faster turnaround time from sample to results
• Better represent tumor heterogeneity

The Oncomine Breast cfDNA Assay v2 enables the analysis of:
• Hotspot genes: AKT1, EGFR, ERBB2, ERBB3, ESR1, FBXW7, KRAS, PIK3CA, SF3B1, TP53 (~152 hotspots)
• Copy number genes (CNVs): CCND1, ERBB2, FGFR1
• Full length genes: TP53 (~80% coverage)

These genes have been identified as frequently mutated in breast cancer. Through the use of Tag Sequencing technology, low limits of detection (LOD) can be achieved for different variant types*:
• For SNVs/short indels, an LOD of 0.1% can be achieved with sensitivity of ~80% and specificity of >99%
• For detection of TP53 mutations, an LOD of 0.5% can be achieved
• For ERBB2 and FGFR1 CNV targets, detection as low as 1.2-fold amplification can be achieved and for CCND1 CNV target, detection as low as 1.4-fold amplification can be achieved with sensitivity of >90% and specificity of >99%

The entire workflow (figure below), from isolation of cfDNA using the MagMAX Cell-Free DNA Isolation Kit (Cat. No. A29319) to analysis of samples, can be accomplished in just two days using the Ion S5 XL sequencing system.

Technology
cfDNA (ctDNA) is found at extremely low concentrations in the plasma fraction of whole blood. Because of this low prevalence, a tag sequencing technology is utilized in this assay. The technology attaches unique molecular tags to the gene-specific primers (figure below). After amplification, the tagged molecules are grouped based on the amplified tags. Groups containing the same mutant variant 80% of the time or greater will be called positive. Using the Tag technology, groups that contain random errors generated through the library construction/sequencing process are removed.

Unlike other technologies with LODs of 1–5%, the Oncomine Breast cfDNA Assay v2 has a flexible detection limit of 0.1% for SNVs or one mutant copy in a background of 1,000 wild-type copies. To achieve 0.1% LOD, 20 ng of input cfDNA is required. Lower amounts of cfDNA can be used, but the %LOD will be higher depending on the input amount (figure below).

Advantages:
• Optimized amplicon design for short cfDNA ensures highest possible capture rate
• Optimized targeted assay design allows highly multiplexed next-generation sequencing (NGS), reducing sequencing costs per sample
• Efficient workflow in just two days

Analysis of SNVs and short indels can be achieved using Torrent Suite Software 5.2 or higher. In order to analyze SNVs, short indels, and CNVs, Ion Reporter 5.6 (cloud- or server-based) is required.

Simplicity, speed, and scalability of Tag Sequencing technology
The Oncomine Breast cfDNA Assay v2 enables cancer genetic studies from just 1 ng of input cfDNA for targeted library construction. The cfDNA Assay uses standard PCR equipment and two simple PCR reactions, one to attach the unique molecular tags and the second to amplify the library, for high multiplex PCR-based target selection. Additionally, the Oncomine Breast cfDNA Assay v2 is compatible with FFPE samples for possible concordance studies. Total time to targeted libraries is just 3.5 hours. Scalability and flexibility are achieved using the Tag Sequencing Barcode Sets 1-24 (Cat. No. A31830) or 25-48 (Cat. No. A31847) for multiplexing barcoded samples on Ion S5 chips.

*Sensitivity and specificity for each variant type were determined using a collection of contrived positive samples and cfDNA isolated from normal healthy donors.

Ion Plus Fragment Library Kit (Ion Torrent™)

As an integral component of the Personal Genome Machine™ sequencing platform, the Ion Plus Fragment Library Kit is designed to produce high quality DNA libraries via multiple possible workflows. The economically priced Ion Plus Fragment Library Kit provides low cost sample preparation, bringing next generation sequencing to every lab. This revolutionary kit allows completion of library preparation in as little as 2 hours for gDNA and amplicon libraries when combined with either physical shearing methods or our proprietary Ion Shear™ enzymatic fragmentation technology (Ion Xpress Plus Library Kit, Cat. No. 4471269), thus saving time and cost. With significantly higher yields than other library construction techniques, high quality libraries can be attained with as little as 100 ng input DNA. In addition, this highly efficient library construction process enables the creation of “amplification-free" libraries from <1 ug input material. This kit is also extremely flexible in that it is compatible with multiple physical shearing and library size selection methods.

The Ion Plus Fragment Library Kit contains sample preparation reagents for library construction of up to 20 DNA libraries (depending on input DNA type and amount) for semiconductor sequencing. Store components at -20°C.

The Ion Plus Fragment Library Kit offers you:
• Lower input requirement with as little as 100 ng starting material
• Rapid and flexible workflow with Ion fragment libraries containing variable insert sizes with a choice of mix-and-match workflows
• Scalable methodology applicable to automated library preparation systems including the AB Library Builder™ System

A Single-Day Workflow: The Next Stage in a Sequencing Revolution
With a quick turnaround time the Ion Plus Fragment Library Kit shortens the Ion sequencing workflow to a single workday, making comprehensive analyses of your samples possible in less time than ever before. Furthermore, the Ion Plus Fragment Library Kit is compatible with a variety of DNA shearing and library size-selection methodologies allowing you to tailor your workflow to suit your project needs.

For research use only. Not intended for human or animal therapeutic or diagnostic use.

Ion PGM™ Wash 2 Bottle Kit (Ion Torrent™)

The Ion PGM™ Wash 2 Bottle Kit is used in conjunction with the Ion PGM™ Hi-Q™ Sequencing Kit. The bottle kit contains conditioning solution and a Wash 2 bottle that may be used for up to 40 runs before replacement. The reusability of this wash bottle allows for not only improved run to run reproducibility but reduced waste and cost savings per run.

Ion Xpress™ Plus Fragment Library Kit (Ion Torrent™)

The Ion Xpress™ Plus Fragment Library Kit provides a rapid and flexible enzymatic-based library construction methodology for the Personal Genome Machine™ sequencing system workflow. Employing enhanced proprietary Ion Shear™ DNA fragmentation chemistry, this revolutionary kit allows completion of library preparation in as little as 2 hours for gDNA and amplicon libraries, removing the need for a physical shearing device to save time and cost. With significantly higher yields and lower bias than other library construction techniques, superior coverage uniformity is attained with as little as 100 ng input DNA. In addition, highly efficient library construction enables the creation of "amplification-free" libraries from 1 µg input material. This kit allows users to modulate an appropriate fragment insert size depending on desired read length and application.

The Ion Xpress™ Plus Fragment Library Kit contains sample preparation reagents for enzymatic shearing and library construction for up to 20 DNA libraries (depending on input DNA type) for semiconductor sequencing. Store components at -20°C.

The Ion Xpress™ Plus Fragment Library Kit offers you:
• Superior coverage uniformity for diverse sample types using our proprietary Ion Shear™ Plus chemistry
• Lower input requirement with as little as 100 ng starting material
• Rapid and flexible workflow with Ion fragment libraries containing variable insert sizes in as fast as 2 hours
• Significant cost savings by removing the need for ancillary shearing devices
• Scalable methodology applicable to automated library preparation systems including the AB Library Builder™ System

A Single-Day Workflow: The Next Stage in a Sequencing Revolution
With a turnaround time of as little as 2 hours, the Ion Xpress™ Fragment Library Kit shortens the Ion sequencing workflow to a single workday, making comprehensive analyses of your sample(s) of interest possible in less time than ever before. Furthermore, Ion Xpress™ Plus fragment libraries exhibit greater coverage uniformity and higher recovery rates across diverse sample types than other library construction methodologies, so you can be confident of generating the best data in the shortest time.

Ion Torrent Sequencing Made Simpler, Faster, and More Affordable
Ion Shear™ Plus DNA fragmentation technology, included in the Ion Xpress™ Plus kit, obviates the need for costly physical shearing devices for most applications and requires as little as 100 ng of sample, accelerating your path to semiconductor sequencing. This flexible technology provides users the ability to adjust the resulting DNA fragment size to accommodate a range of sequencing read lengths depending on their project requirements. This technology is also amenable to automated library preparation systems such as the AB Library Builder™ System to provide quick answers when you need them most. Ion Shear™ Plus DNA fragmentation technology is not included in the Ion Plus kit. Use the Ion Plus kit if physical shearing methods are preferred.

For Research Use Only. Not intended for human or animal therapeutic or diagnostic use.

Ion AmpliSeq™ Library Kit Plus (Ion Torrent™)

The Ion AmpliSeq Library Kit Plus is designed for rapid preparation of amplicon libraries using Ion AmpliSeq panels. Ion AmpliSeq technology enables scalable, multiplex polymerase chain reactions (PCR) from 12- to 24,000-plex in a single well. Next-generation sequencing (NGS) libraries can be prepared using as little as 1 ng of starting material.

Key product features:
• Scalable and efficient library preparation
• Easy and robust protocol
• Barcoded sample multiplexing

Scalable and efficient library preparation
Using as little as 1 ng of starting material, highly multiplexed PCR amplification of thousands of genomic target regions yields superior coverage uniformity and specificity, without the need of a specialized microfluidics PCR platform. The primers contain proprietary modifications that enable removal of primer sequences during library preparation, thereby resulting in efficient target assessment during sequencing.

Easy and robust protocol
The Ion AmpliSeq Library Kit Plus uses a plate-based format for easier sample handling and tracking, and for compatibility with automation and high-throughput laboratories. The protocol is simple to use and enables high yield and uniformity, reliably and reproducibly. The resulting libraries are ready for downstream template preparation using the automated Ion Chef System (which also provides the option of automated library preparation using the Ion AmpliSeq Kit for Chef DL8) or the Ion OneTouch 2 System followed by sequencing. The intuitive Torrent Suite Software and optional Ion Reporter Software enables automation of data analysis.

Barcoded sample multiplexing
The Ion AmpliSeq Library Kit Plus includes reagents for generating amplicons using Ion AmpliSeq primers and preparing libraries from the resulting amplicons. The kit enables the preparation of barcoded libraries using Ion Xpress Barcode Adapters 1-96 kits or IonCode Barcode Adapters 1-384 kits. Barcoded libraries can be combined and loaded onto a single Ion chip to minimize the sequencing run time and cost, and to allow for accurate sample-to-sample comparisons.

BigDye™ Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems™)

The BigDye® Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, Plasmid, Fosmid, and BAC templates.
• Improve the quality of your results for a wide range of sequencing applications.
• Optimized for long read lengths.
• Better dye mobility characteristics
• Improved performance reading through GT rich regions.
• Get longer, higher-quality reads with more uniform peak heights and optimal signal balance.
• Enhance your productivity and reduce costs.

Get More Uniform Peak Heights, Improved Accuracy
With BigDye® Terminator v3.1 kit superior chemistry, you generate data with uniform peak heights and optimized signal balance. This results in longer, higher-quality reads and more accurate base assignments for heterozygote and mutation detection.

For Research Use Only. Not for use in diagnostics procedures.

ClaSeek Library Preparation Kit, Illumina compatible (Thermo Scientific™)

Thermo Scientific ClaSeek Library Preparation Kit, Illumina™ compatible, is designed for fast and convenient construction of PCR-free NGS fragment library from DNA sample input as low as 500 ng. In combination with the amplification protocol the kit also enables the generation of DNA fragment libraries from ultra-low 5 ng sample input.

The kit utilizes a highly efficient NGS-optimized library construction protocol combining end-conversion and adapter addition steps into a simple and convenient one-tube procedure. This minimizes unnecessary pipetting steps and reduces hands-on time, allowing for PCR-free library construction in less than 70 minutes. The kit is suitable for construction of 50, 300, 500 bp read-length Illumina™-compatible libraries and is validated for use on Illumina™ HiSeq™ and MiSeq™ sequencing platforms.

Highlights

PCR-free—significantly minimized bias and improved coverage with amplification-free protocol
Flexible—universal protocol for variable sample input (5 ng—1 µg) and DNA fragment lengths
Fast and Convenient—fewer cleanup steps, less hands-on time, optimized master mixes, one-tube end conversion procedure.

Applications

DNA fragment library preparation for downstream use in next generation sequencing workflow.

Related Products
ClaSeek Library Preparation Kit, Illumina compatible

Ion 540™ Kit for Agrigenomics—Chef (Ion Torrent™)

The Ion 540 Kit-Chef enables robust and automated template preparation and sequencing of up to 200 base-read libraries using the Ion Chef and the Ion S5 or Ion S5 XL Sequencing Systems. The Ion 540 Kit contains pre-packaged single-use template and sequencing reagent cartridges with integrated sample tracking, delivering an automated workflow for sequencing of 8 loaded chips with complete run traceability.

The Ion 540 Kit for the Ion Chef and Ion S5/Ion S5 XL workflow:

• Provides template preparation and chip loading reagents for up to 200 base-read libraries
• Simplifies your research with ready-to-use, disposable reagent cartridges for sequencing
• Enables fast and simple sequencing instrument set-up, with less than 15 minutes hands-on time
• Leverages the latest advancements in data quality and accuracy

The Ion S5/Ion S5 XL sequencers, along with the Ion Chef system, offer reproducible sequencing workflows. Delivering up to 80 million reads in a single run, the Ion 540 Chip empowers rapid exome and transcriptome analysis. Combined with Ion AmpliSeq technology and Ion Reporter Software for data interpretation, the Ion S5/Ion S5 XL systems enable a cost effective and comprehensive solution for your translational or inherited disease research needs.

Ion GeneStudio™ S5 Food Protection System (Thermo Scientific™)

Identify species present in all food types using this next generation sequencing instrument as part of a NGS Workflow for Food Authenticity Workflow.

Ion AmpliSeq™ Transcriptome Human Gene Expression Kit (Ion Torrent™)

The Ion AmpliSeq™ Transcriptome Human Gene Expression Kit enables the simultaneous measurement of the expression levels of over 20,000 human RefSeq genes in a single assay. Providing the ability to work directly with low input levels of total RNA derived from challenging samples such as formalin-fixed paraffin-embedded (FFPE) tissue or other samples with degraded RNA, the Ion AmpliSeq™ Transcriptome Human Gene Expression Kit is ideal for those interested in obtaining comprehensive, highly sensitive, and easily obtained gene expression measurements from a wide range of RNA sources. For automated library production, see the Ion AmpliSeq Transcriptome Human Gene Expression Panel, Chef-ready Kit.

The Ion AmpliSeq™ Transcriptome Human Gene Expression Kit offers:
• Flexible, straightforward library preparation – start with as little as 10 ng of total RNA from FFPE samples, cells, or other sources
• Representation of over 20,000 human RefSeq genes
• Wider dynamic range and improved detection sensitivity compared to conventional microarray analysis
• A complementary software plug-in for automated sequencing analysis that provides gene quantitation and reporting as well as quality control metrics
• Compatibility with Ion Xpress™ Barcode adapters allowing for multiplexed sequencing

Wider dynamic range for enhanced performance
With a wider dynamic range and improved gene detection compared to conventional microarray analysis, the Ion AmpliSeq Transcriptome™ Human Gene Expression Kit provides a clear solution to researchers needing enhanced performance for their gene expression needs. A simple workflow allows for sample-to-gene quantitation in less than 2 days, helping to reduce workload and increase throughput.

Straightforward library preparation and multiplex sequencing
Starting with as little as 10 ng of total RNA, libraries can be prepared in approximately 7 hours with only one hour of hands-on time. Total RNA is first processed with the included SuperScript® VILO™ cDNA Synthesis kit, and the resulting cDNA is amplified using Ion AmpliSeq™ technology to accurately maintain expression levels of all genes.

The resulting libraries are ready for downstream template preparation using the Ion OneTouch™ 2 System or Ion Chef™ System and sequencing on the Ion Proton™ sequencing system. Libraries can be barcoded for multiplexing, allowing for flexibility, helping to reduce cost, and increase throughput, with the recommended 8 libraries per Ion PI™ chip. Other multiplexing options are also possible, depending on the experimental design and desired level of sensitivity.

Automatic data analysis with Torrent Suite™ plug-in
Using the complementary analysis plug-in available with Torrent Suite™ software, gene quantitation information is automatically compiled, minimizing the requirement for costly computational resources and bioinformatics expertise to process raw sequencing data into meaningful information. An easy to interpret, highly portable table is produced containing all genes interrogated and their respective expression levels, output that may be used with a variety of existing software applications for downstream analysis of microarray or next-generation sequencing data.

Learn more about the Ion AmpliSeq Transcriptome Human Gene Expression Kit ›

Note: Libraries made from this protocol yield fragments suitable for 200-base read sequencing.

Precision ID mtDNA Control Region Panel (Applied Biosystems™)

The Applied Biosystems Precision ID mtDNA Control Region Panel is an innovative next-generation sequencing (NGS) approach to mitochondrial DNA (mtDNA) analysis specifically developed for forensic applications. It is a 2-pool multiplex assay that targets the 1.2 kb control region of the human mitochondrial genome, which encompasses HV-I,II, and III (see figure below). This panel was constructed using a novel, innovative tiling approach to obtain optimal coverage data from highly compromised, degraded samples such as hair shafts, teeth, and bones that uses massively parallel sequencing built on Ion AmpliSeq technology. This mtDNA tiling approach was also used to construct the Precision ID mtDNA Whole Genome Panel (Cat. No. A30938).Simplicity
• Small amplicons enable control region coverage of degraded samples
• As little as 2 pg of input
• Automated library and template preparation using the Ion Chef System
• Optimized analysis using Converge software

Scalability
• Multiple chip formats to meet a range of sample throughput requirements
• Ability to multiplex up to 56 samples*

Speed
• Less than 45 minutes of hands-on time for a DNA-to-data targeted sequencing workflow*
• Total sample-to-data time of less than 2 days*

Mitochondrial DNA analysis is a key DNA detection method used by forensic scientists when samples such as teeth, bones, and hairs without a follicle (root) are collected from crime scenes, missing person remains, or disaster areas that have been exposed to high environmental stress where nuclear DNA is insufficient in quality and/or quantity. Balanced coverage is achieved using a small amplicon design, optimized with the inclusion of degenerate primers in the Precision ID mtDNA Control Region Panel, which increases success with degraded and low template DNA samples. This panel can be used with all types of forensic samples, including ancient samples as well as clinical research specimens.

Additionally, full automation of library and template preparation of the Precision ID mtDNA Control Region Panel using the Ion Chef System and sequencing on the Ion GeneStudio series systems can reduce variation observed with manual workflows and streamline user-instrument interaction with less than 45 minutes of hands-on time. The Converge NGS Analysis module automates mtDNA analysis, leveraging optimized base calling, phylogenetically-guided alignment, and quality filtering algorithms specific for the Precision ID mtDNA panels. Variant calling including, including heteroplasmy detection can be easily optimized within the software features. These solutions give forensic DNA laboratories the flexibility to detect variation within noncoding control sequences using the Precision ID mtDNA Control Region Panel or take advantage of the genetic diversity of full mtGenome sequence data using the Precision ID mtDNA Whole Genome Panel.

NOTE: The Precision ID mtDNA Control Region Panel provides sufficient material to perform 96 reactions using the manual Precision ID Library kit (Cat. No. A26435) or 48 reactions using the automated Precision ID DL8 Library Kit (Cat. No. A33212).

* Using the Precision ID NGS System for Human Identification

Sanger Sequencing Kit (Applied Biosystems™)

The Applied Biosystems Sanger Sequencing Kit includes ExoSAP-IT Express reagent, a BigDye Terminator v3.1 Cycle Sequencing Kit, and a BigDye XTerminator Purification Kit, providing all the reagents needed to clean up PCR products, perform cycle sequencing, and clean up sequencing products. This simple, fast workflow enables completion of Sanger sequencing, from sample to answer, in less than a workday.

ExoSAP-IT Express reagent: fastest PCR cleanup method
In only 5 minutes, ExoSAP-IT Express PCR Product Cleanup Reagent removes excess primers and unincorporated nucleotides from a PCR reaction. Eliminate spin columns, magnetic beads, sedimentation, filtration, and gel purification. The novel ExoSAP-IT technology allows for a significant reduction in sample cleanup time with minimal steps, providing the simplest workflow.

• 5-min protocol
• One-tube, one-step PCR cleanup
• Novel enzyme technology
• 100% recovery of PCR products

BigDye Terminator v3.1 Cycle Sequencing Kit
The BigDye Terminator v3.1 Cycle Sequencing Kit provides premixed reagents for Sanger sequencing reactions. The kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, plasmid, fosmid, and BAC templates.

• Improve the quality of your results for a wide range of sequencing applications
• Optimized for long read lengths
• Better dye mobility characteristics
• Improved performance reading through GT-rich regions
• Get longer, higher-quality reads with more uniform peak heights and optimal signal balance
• Enhance your productivity and reduce costs

BigDye XTerminator Purification Kit
The BigDye XTerminator Purification Kit is a fast, simple purification method for DNA sequencing reactions that removes unincorporated BigDye terminators and salts. No more dye blobs! Cleanup is complete in under 40 minutes and requires less than 10 minutes of labor.

• Eliminate liquid transfer—use a single plate
• Obtain complete dye blob removal
• Stabilize samples before analysis
• Improve your sequencing workflow
• Perform manual or automated protocols
• Get rapid, reliable, and reproducible results

Ion 550™ Kit for Agrigenomics—Chef (Ion Torrent™)

The Ion 550 Kit enables robust and automated template preparation and sequencing of up to 200 base-read libraries using the Ion Chef System and the Ion GeneStudio S5 Plus, Ion GeneStudio S5 Prime or Ion S5 XL sequencing systems.

The Ion 550 Kit contains pre-packaged template and sequencing reagent cartridges with integrated sample tracking, delivering an automated workflow for sequencing of eight loaded chips with complete run traceability.

The Ion 550 Kit, together with the Ion 550 Single Chip Supplemental Kit, enables the flexibility to prepare one or two loaded chips per Ion Chef run, without loss of templating reagents.

The Ion 550 Chip workflow:
• Provides template preparation and chip loading reagents for up to 200 base–read libraries
• Maximizes flexibility with the ability to load one or two Ion 550 chips per Ion Chef run
• Simplifies your research with ready-to-use, disposable reagent cartridges
• Delivers fast and simple sequencing instrument set-up, with less than 15 minutes hands-on time
• Leverages the latest advancements in data quality and accuracy

The Ion GeneStudio S5 Plus and S5 Prime systems, along with the Ion Chef Instrument, provide reproducible sequencing workflows. Delivering up to 130 million reads in a single run, the Ion 550 Chip empowers rapid exome and transcriptome analysis, as well as high coverage for cell free nucleic acid applications. Combined with Ion AmpliSeq technology and Ion Reporter Software for data interpretation, the Ion 550 Chip workflow provides a cost-effective and comprehensive solution for your inherited disease or oncology research needs.

Ion Chef™ Food Protection Instrument (Thermo Scientific™)

Simplify the NGS Food Authenticity Workflow by automating the template preparation and chip loading steps with the Ion Chef™ Food Protection Instrument.