Shop All Conjugated Biomolecules

BODIPY™ TR Ceramide complexed to BSA (Invitrogen™)

The red-fluorescent BODIPY® TR C5 ceramide complexed to BSA can be used in sphingolipid transport and metabolism mechanisms. Complexing fluorescent lipids with bovine serum albuin (BSA) facilitates cell labeling by eliminating the need for organic solvents to dissolve the lipophilic probe - the BSA-complexed probe can be directly dissolved in water.

BODIPY™ TR Ceramide (Invitrogen™)

BODIPY TR ceramide is a red-fluorescent dye that can be used to stain Golgi apparatus in live cells.

BODIPY™ FL C5-Ganglioside GM1 complexed to BSA (Invitrogen™)

Colocalization of fluorescent cholera toxin B conjugates and the green-fluorescent BODIPY® FL C5-ganglioside GM1 complexed to BSA observed by fluorescence microscopy provides a direct indication of the association of these molecules in lipid rafts. Complexing fluorescent lipids with bovine serum albuin (BSA) facilitates cell labeling by eliminating the need for organic solvents to dissolve the lipophilic probe - the BSA-complexed probe can be directly dissolved in water.

pHrodo™ Red Epidermal Growth Factor (EGF) Conjugate (Invitrogen™)

pHrodo Red Epidermal Growth Factor (EGF) Conjugate is used for the detection of internalized EGF receptor in cells. Fluorescently labeled EGF has enabled scientists to investigate receptor-membrane interactions, study receptor distribution, calculate rate constants for the interaction of EGF with its receptor, and more. Fluorescent labels that respond to a pH change add another level of detection. pHrodo Red is weakly fluorescent outside of cells at neutral pH, but when internalized specifically through EGF endocytosis, it becomes brightly fluorescent in the acidic endosomes. This allows the measurement of internalized EGF, as well as the dynamic monitoring of EGF internalization in live cells.

Features of this EGF conjugate include:

• pHrodo Red dye conjugated directly to highly active human recombinant EGF molecule
• Excitation/emission of 560/585 nm
• Excellent photostability
• Appropriate for multiplexing with green fluorophores such as GFP, FITC, and Alexa Fluor 488

pHrodo Red Epidermal Growth Factor (EGF) Conjugate is compatible with various platforms, such as traditional fluorescence microscopy, high content screening (HCS), flow cytometery, and microplate-based fluorimetry or high throughput screening (HTS). This reagent is also compatible with various benchtop instruments such as the Evos, FLoid, Tali, and Attune instruments.

Wheat Germ Agglutinin, Tetramethylrhodamine Conjugate (Invitrogen™)

Wheat germ agglutinin (WGA) is one of the most widely used lectins in cell biology. Our tetramethylrhodamine conjugate of WGA exhibits the bright, orange fluorescence of the tetramethylrhodamine dye (excitation/emission maxima ~555/580 nm). Tetramethylrhodamine WGA binds to sialic acid and N-acetylglucosaminyl residues.

BODIPY™ FL C12-Sphingomyelin (N-(4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Dodecanoyl) Sphingosyl Phosphocholine) (Invitrogen™)

The green-fluorescent sphingomyelin, BODIPY® FL C12-sphingomyelin can be used to study sphingolipid transport and signaling pathway in cells.

CaptureSelect™ Biotin Anti-IgG-Fc (Multi-species) Conjugate (Thermo Scientific™)

CaptureSelect™ Biotin Anti-IgG-Fc (ms) Conjugate consists of a 13 kDa llama antibody fragment (affinity ligand) that specifically binds to the Fc part of IgG from multiple species. The affinity ligand is chemically conjugated to biotin via an appropriate spacer that retains the binding reactivity of the ligand when used in combination with streptavidin-based conjugates or streptavidin pre-coated surfaces.

The CaptureSelect™ Biotin Anti-IgG-Fc (ms) Conjugate allows you to:

Detect, quantitate, and characterize IgG antibodies from multiple species (all subclasses, IgG-specific), multi-species IgG-derived Fc fusion proteins, recombinant IgG antibodies, and total IgG in plasma and/or serum samples
Avoid cross-binding with other isotypes, such as IgA and IgM

Applications for CaptureSelect™ Biotin Anti-IgG-Fc (ms) Conjugate include ELISA, Western blot, Gyrolab™-based immunoassays, and label-free detection platforms such as those based on surface plasmon resonance (Biacore™ and IBIS-MX96 systems) and bio-layer interferometry (ForteBio™ Octet™ systems).

BODIPY™ FL C5-Lactosylceramide complexed to BSA (Invitrogen™)

The green-fluorescent BODIPY® FL C5 lactosylceramide complexed to BSA can be used in sphingolipid transport and metabolism mechanisms. Complexing fluorescent lipids with bovine serum albuin (BSA) facilitates cell labeling by eliminating the need for organic solvents to dissolve the lipophilic probe - the BSA-complexed probe can be directly dissolved in water.

CaptureSelect™ Biotin Anti-C3 Conjugate (Thermo Scientific™)

CaptureSelect™ Biotin Anti-C3 Conjugate consists of a 13 kDa camelid antibody fragment (affinity ligand) that specifically binds with high affinity and selectivity to human complement component 3 (C3). The affinity ligand is chemically conjugated to biotin via an appropriate spacer that retains the binding reactivity of the ligand when used in combination with streptavidin-based conjugates or streptavidin pre-coated surfaces. This conjugate allows you to detect, quantitate, and characterize native and recombinant human C3.

Applications for CaptureSelect Biotin Anti-C3 Conjugate include ELISA, immunoprecipitation with streptavidin-coupled Dynabeads™, MSIA Streptavidin D.A.R.T.’S, Gyrolab™-based immunoassays, and label-free detection platforms such as those based on surface plasmon resonance (Biacore™ and IBIS-MX96 systems) and bio-layer interferometry (ForteBio™ Octet™ systems).

Concanavalin A, Tetramethylrhodamine Conjugate (Invitrogen™)

Concanavalin A (Con A) is one of the most widely used lectins in cell biology. Our tetramethylrhodamine conjugate of Con A exhibits the bright, orange-red fluorescence of tetramethylrhodamine (absorption/emission maxima ~555/580 nm). Tetramethylrhodamine Con A selectively binds to α-mannopyranosyl and α-glucopyranosyl residues.

pHrodo™ Green Epidermal Growth Factor (EGF) Conjugate (Invitrogen™)

pHrodo Green Epidermal Growth Factor (EGF) Conjugate is used for the detection of internalized EGF receptor in cells. Fluorescently labeled EGF has enabled scientists to investigate receptor-membrane interactions, study receptor distribution, calculate rate constants for the interaction of EGF with its receptor, and more. Fluorescent labels that respond to a pH change add another level of detection. pHrodo Green is weakly fluorescent outside of cells at neutral pH, but when internalized specifically through EGF endocytosis, it becomes brightly fluorescent in the acidic endosomes. This allows the measurement of internalized EGF, as well as the dynamic monitoring of EGF internalization in live cells.

Features of this EGF conjugate include:

• pHrodo Green dye conjugated directly to highly active human recombinant EGF molecule
• Excitation/emission of 509/533 nm
• Excellent photostability
• Appropriate for multiplexing with red and deep red fluorophores such as RFP, Alexa Fluor 594, MitoTracker Red, and MitoTracker Deep Red

pHrodo Green Epidermal Growth Factor (EGF) Conjugate is compatible with various platforms, such as traditional fluorescence microscopy, high content screening (HCS), flow cytometery, and microplate-based fluorimetry or high throughput screening (HTS). This reagent is also compatible with various benchtop instruments such as the Evos, FLoid, Tali, and Attune instruments.

CaptureSelect™ Biotin Anti-AAT Conjugate (Thermo Scientific™)

CaptureSelect™ Biotin Anti-AAT Conjugate consists of a 13 kDa camelid antibody fragment (affinity ligand) that specifically binds with high affinity and selectivity to human alpha-1 antitrypsin (AAT). The affinity ligand is chemically conjugated to biotin via an appropriate spacer that retains the binding reactivity of the ligand when used in combination with streptavidin-based conjugates or streptavidin pre-coated surfaces. This conjugate allows you to detect, quantitate, and characterize native and recombinant human AAT.

Applications for CaptureSelect Biotin Anti-AAT Conjugate include ELISA, immunoprecipitation with streptavidin-coupled Dynabeads™, Gyrolab™-based immunoassays, and label-free detection using platforms such as those based on surface plasmon resonance (Biacore™ and IBIS-MX96 systems) and bio-layer interferometry (ForteBio™ Octet™ systems).

CaptureSelect™ Biotin Anti-FIX Conjugate (Thermo Scientific™)

CaptureSelect™ Biotin Anti-FIX Conjugate consists of a 13 kDa camelid antibody fragment (affinity ligand) with high affinity and selectivity for both recombinant and plasma derived human Factor IX (FIX). The affinity ligand is chemically conjugated to biotin via an appropriate spacer that retains the binding reactivity of the ligand when used in combination with streptavidin-based conjugates or streptavidin pre-coated surfaces.

The CaptureSelect™ Biotin Anti-FIX Conjugate allows you to:

Detect, quantitate, and characterize recombinant and plasma derived human FIX

Applications for CaptureSelect™ Biotin Anti-FIX Conjugate include ELISA, Western blot, Gyrolab™-based immunoassays, and label-free detection platforms such as those based on surface plasmon resonance (Biacore™ and IBIS-MX96 systems) and bio-layer interferometry (ForteBio™ Octet™ systems).

Dextran, Texas Red™, 10,000 MW, Neutral (Invitrogen™)

Labeled dextrans are hydrophilic polysaccharides most commonly used in microscopy studies to monitor cell division, track the movement of live cells, and to report the hydrodynamic properties of the cytoplasmic matrix. The labeled dextran is commonly introduced into the cells via microinjection.

Need a different emission spectrum or longer tracking? View our other mammalian cell tracking products.

Dextran Specifications:

Label (Ex/Em): Texas Red® (595/615)
Size: 10,000 MW
Charge: Zwitterionic
Fixable: Nonfixable

High Manufacturing Standards of Molecular Probes® Dextrans
We offer more than 50 fluorescent and biotinylated dextran conjugates in several molecular weight ranges. Dextrans are hydrophilic polysaccharides characterized by their moderate-to-high molecular weight, good water solubility, and low toxicity. They also generally exhibit low immunogeniticy. Dextrans are biologically inert due to their uncommon poly-(α-D-1,6-glucose) linkages, which render them resistant to cleavage by most endogenous cellular glycosidases.

In most cases, Molecular Probes® fluorescent dextrans are much brighter and have higher negative charge than dextrans available from other sources. Furthermore, we use rigorous methods for removing as much unconjugated dye as practical, and then assay our dextran conjugates by thin-layer chromatography to help ensure the absence of low molecular weight contaminants.

A Wide Selection of Substituents and Molecular Weights
Molecular Probes® dextrans are conjugated to biotin or a wide variety of fluorophores, including seven of our Alexa Fluor® dyes (Molecular Probes dextran conjugates–Table 14.4) and are available in these nominal molecular weights (MW): 3,000; 10,000; 40,000; 70,000; 500,000; and 2,000,000 daltons.

Dextran Net Charge and Fixability
We employ succinimidyl coupling of our dyes to the dextran molecule, which, in most cases, results in a neutral or anionic dextran. The reaction used to produce the Rhodamine Green™ and Alexa Fluor® 488 dextrans results in the final product being neutral, anionic, or cationic. The Alexa Fluor®, Cascade Blue®, lucifer yellow, fluorescein, and Oregon Green® dextrans are intrinsically anionic, whereas most of the dextrans labeled with the zwitterionic rhodamine B, tetramethylrhodamine, and Texas Red® dyes are essentially neutral. To produce more highly anionic dextrans, we have developed a proprietary procedure for adding negatively charged groups to the dextran carriers; these products are designated "polyanionic" dextrans.

Some applications require that the dextran tracer be treated with formaldehyde or glutaraldehyde for subsequent analysis. For these applications, we offer "lysine-fixable" versions of most of our dextran conjugates of fluorophores or biotin. These dextrans have covalently bound lysine residues that permit dextran tracers to be conjugated to surrounding biomolecules by aldehyde-mediated fixation for subsequent detection by immunohistochemical and ultrastructural techniques. We have also shown that all of our 10,000 MW Alexa Fluor® dextran conjugates can be fixed with aldehyde-based fixatives.

Key Applications Using Labeled Dextrans
There are a multitude of citations describing the use of labeled dextrans. Some of the most common uses include:

Neuronal tracing (anterograde and retrograde) in live cells
Cell lineage tracing in live cells
Neuroanatomical tracing
Examining intercellular communications (e.g., in gap junctions, during wound healing, and during embryonic development)
Investigating vascular permeability and blood–brain barrier integrity
Tracking endocytosis
Monitoring acidification (some dextran–dye conjugates are pH-sensitive)
Studying the hydrodynamic properties of the cytoplasmic matrix

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

FL - CBP-1 Peptide, 100µM

LanthaScreen® fluorescent nuclear receptor coregulator peptides contain known interaction motifs and are labeled with fluorescein. These peptides are matched and validated to complement the LanthaScreen® TR-FRET Nuclear Receptor Coregulator Assays. Assays developed using these reagents enable primary or secondary screening of nuclear receptor agonists and/or antagonists.

How they work
Ligand binding to nuclear receptors causes conformational changes in the receptor, resulting in a cascade of events, including dissociation of repressor proteins, association of coactivator proteins, and assembly of pol II and other transcriptional factors for activation of target genes. TR-FRET based assays can be developed using the LanthaScreen® panel of fluorescein-labeled coregulator peptides to investigate conformational changes of nuclear receptors upon ligand binding, either by determining the affinity of ligand-bound receptor for different coregulator peptides, or by identifying additional agonists or antagonists via displacement or recruitment of a specific coregulator peptide. In the LanthaScreen® TR-FRET RXR beta Coactivator Assay, a terbium (Tb)-labeled anti-GST antibody is used to indirectly label GSTtagged RXR beta protein. An agonist (9-cis Retinoic Acid) added to the receptor which, upon ligand binding, causes a conformational change resulting in recruitment of the Fl-D22 coactivator peptide (Figure 1). The binding of the fluorescent coregulator peptide to RXR beta causes an increase in the TR-FRET emission ratio (Figure 2).

Contents and Storage:

LanthaScreen® fluorescent nuclear receptor coregulator peptides are supplied as 100 µM solutions in peptide-appropriate buffers. Store peptides at -20°C.