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Sterilin™ Borosilicate Glass Culture Tubes Thermo Scientific™
NOT AVAILABLE IN AMERICA
Predilution Test Tubes Thermo Scientific™
Prepare a large sample volume for subsequent transfer to microplates with Thermo Scientific™ Predilution Test Tubes.
Features of Peptide Retention Time Calibration Mixture:
• Assess chromatography and MS instrument performance
• Predict peptide retention across multiple instrument platforms
• Predict peptide retention time from sequence using calculated hydrophobicity factor
• Optimize scheduled MS acquisition windows for improved quantification and increased multiplexing
• Normalize collision energies between instruments and platforms
• Normalize results for variation in retention times and peak intensities between runs
The Pierce Peptide Retention Time Calibration Mixture is used to optimize and assess LC parameters, identify the total peptide elution window, and optimize MS parameters. Testing the mixture frequently throughout the lifetime of a column or including it in quantitative samples makes it possible to monitor and normalize LC-MS performance between samples and over time. When used with Thermo Scientific Pinpoint 1.1 software, the calibration mixture can predict peptide retention times based upon peptide sequence or relative retention time between instruments. The mixture can also be used with Pinpoint software to establish normalized collision energies between platforms. This allows normalization of methods and eases the transfer of quantitative methods between LC-MS platforms. Pinpoint software, sample files, tutorials, and videos can be downloaded from the Thermo Proteomics Portal.
The Pierce Peptide Retention Time Calibration Mixture contains 15 synthetic heavy peptides mixed at an equimolar ratio that elute across the chromatographic gradient. The observed retention times and hydrophobicity factors (HF) for these calibrant peptides are fit to a linear equation to determine the slope of the retention time/HF relationship. This equation and the HF of an uncharacterized peptide are then used to predict retention time.
The prediction of peptide retention time assists in the development of multiplexed, high throughput mass spectrometric assays. The Pierce Peptide Retention Time Calibration Mixture and Thermo Scientific Pinpoint software streamline the transition from qualitative protein discovery results to the development of targeted MS assays on Thermo Scientific triple quadrupole, Orbitrap, Exactive, and ion trap mass spectrometers. The Pierce Peptide Retention Time Calibration Mixture permits optimization and regular assessment of chromatographic performance and for rapid development of multiplexed, scheduled targeted MS assays for the quantification of dozens to hundreds of peptide targets per run.
More Product Data
• Optimize liquid chromatography with a peptide retention time calibration mixture
Silanized Disposable Culture Tubes Thermo Scientific™
Save time and minimize waste costs when performing quantitative analysis or storing materials. Thermo Scientific™ Silanized Disposable Culture Tubes are pre-processed with a silanized surface deactivation treatment, and are offered in convenient, ready-to-use clean packs. Surface deactivation treatments such as silanization may play an important role in preserving the integrity of certain materials or extracts stored in glass containers. It also helps to avoid alkalinization of contents which may occur as carbonates leach from the glass with normal weathering.