PCR Specific Buffers

PCR Specific Buffers

Buffers optimized for specific PCR procedures. Products are available in various volumes and concentrations and for different PCR-related processes.
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Taq DNA Polymerase PCR Buffer is a 10X buffer [200 mM Tris HCl (pH 8.4), 500 mM KCl] supplied with 1 mL of 50 mM MgCl2.
PCRX Enhancer System is an optimized buffer and cosolvent system designed for use in conjunction with DNA polymerases to optimize PCR of problematic and/or GC-rich templates. 10X PCRX Enhancer Solution with 10X PCRX Amplification Buffer offers higher primer specificity, broader magnesium...
Thermo Scientific 10X Taq Buffers are ready-to-use buffers for PCR using Taq DNA polymerases (both recombinant and native). They are available in different compositions and also without detergents.
Thermo Scientific PCR reagents meet high quality standards to generate accurate results from even the most challenging molecular biology applications.
GeneAmp™ 10X PCR Buffer Applied Biosystems™
Applied Biosystems™ GeneAmp™ 10X PCR Buffer I is optimized for use with AmpliTaq™ DNA Polymerase, resulting in robust PCR amplification.
The Invitrogen Buffer Kit provides high quality, RNase free buffers for critical RNA analysis procedures.
DreamTaq™ Buffer (10X) Thermo Scientific™
Thermo Scientific DreamTaq Buffer (10X), available in colorless and green formats, is specifically optimized for PCR using DreamTaq DNA Polymerase and DreamTaq Hot Start DNA Polymerase. It contains both KCl and (NH4)2SO4 to provide high specificity of primer annealing at a wide range of MgCl2...
Invitrogen™ SuperFi™ Buffer is a 5X buffer solution optimized for use with Invitrogen™ Platinum™ SuperFi DNA Polymerase. The buffer contains 7.5 mM MgCl2 which provides 1.5 mM MgCl2 in the final reaction.
Guanidine thiocyanate, 99% Thermo Scientific Chemicals
Guanidine thiocyanate is a useful tool for RNA isolation and protein solubilization. It is involved in the quantification of mRNA from hepatocytes. It acts as a medium for blood smaple lystae in order to maintain the integrity of nucleic acid.
The Max Bacterial Enhancement Reagent is designed for use with TRIzol™ Reagent to improve the isolation of intact total RNA up to three-fold from Gram-positive and Gram-negative bacteria.
Phusion GC Buffer Pack Thermo Scientific™
The error rate of Phusion DNA Polymerase in HF Buffer (4.4 x 10-7) is lower than that in GC Buffer (9.5 x 10-7). Therefore, the HF Buffer should be used as the default buffer for high-fidelity amplification.
SuperScript IV RT Reaction Buffer is included with, and designed for use with, SuperScript IV Reverse Transcriptase.
Phire Green Reaction Buffer Thermo Scientific™
Thermo Scientific Phire Reaction Buffer is designed for Phire Hot Start II DNA Polymerase. This 5X buffer solution is available in three formats, a non-colored version with or without detergents and a green version that contains a density reagent and two tracking dyes for direct loading of PCR...
Yellow Sample Buffer (40X) Thermo Scientific™
40X Yellow Sample Buffer is used to color and track the samples while pipetting and setting up qPCR reactions.
SSC buffer (20X) Thermo Scientific Chemicals
SSC buffer is suitable for preparing nucleic acids for hybridization, hybridization buffer for northern and southern blots, washing buffer for northern and southern blots. Saline-Sodium Citrate Buffer (SSC) is used as a hybridization buffer in immunoblotting and DNA Microarray protocols.
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PCR (Polymerase Chain Reaction) is used in a wide variety of applications including cloning, gene expression analysis, genotyping, sequencing, and mutagenesis. The Thermo Scientific PCR portfolio has PCR and qPCR consumables for every successful PCR run: thermal cyclers, plates, tubes, sealing...
Ribonuclease- (RNase-) free buffers and reagents are rigorously tested for nuclease contamination (RNase and DNase) as part of the routine quality control process. These reagents have all been shown to lack detectable non-specific endonucleases, exonucleases, and RNase activity.
Documents & Support (1,381)
How many freeze cycles do you recommend for Applied Biosystems, GeneAmp 10X PCR Buffer I (Cat. No. 4379876)?
With Platinum II Taq Hot-Start DNA Polymerase and Platinum II PCR buffer, how is it possible to use an annealing temperature of 60 degrees C for any primer pair?