PCR Specific Buffers

PCR Specific Buffers

Buffers optimized for specific PCR procedures. Products are available in various volumes and concentrations and for different PCR-related processes.
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UltraPure™ Glycerol Invitrogen™

UltraPure Glycerol is suitable for the concentration and storage of enzymes at low temperatures. A 50% (v/v) solution will not freeze at -20°C. Because it has a density of 1.26 g/mL, glycerol frequently is used as a component in electrophoresis loading buffers.

UltraPure™ Guanidine Isothiocyanate Solution Invitrogen™

UltraPure™ Guanidine Isothiocyanate Solution consists of 4 M guanidine isothiocyanate, 50 mM Tris-HCl (pH 7.5), 25 mM EDTA. It may be used as part of a protocol, based on the guanidine isothiocyanate procedure first described by Chirgwin et al. (1) and later modified by Han et al.

SSC buffer (20X) Thermo Scientific™

This Thermo Scientific brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific.

Saline-sodium Citrate, 20x Solution Thermo Scientific™

This Thermo Scientific brand product was originally part of the Acros Organics product portfolio. Some documentation and label information may refer to the legacy brand. The original Acros Organics product / item code or SKU reference has not changed as a part of the brand transition to Thermo...

Guanidine thiocyanate, 99% Thermo Scientific™

This Thermo Scientific brand product was originally part of the Alfa Aesar product portfolio. Some documentation and label information may refer to the legacy brand. The original Alfa Aesar product / item code or SKU reference has not changed as a part of the brand transition to Thermo Scientific.

QuantumRNA™ Classic II 18S Internal Standard Invitrogen™

The Ambion® QuantumRNA™ 18S Internal Standard with Competimer™ technology uses specially modified Competimer™s of the same sequence as the normal 18S primers that cannot be extended. By adjusting the ratio of 18S Competimer™s to normal 18S mRNA primers, the signal for 18S mRNA can be attenuated to...

Yeast tRNA (10 mg/mL) Invitrogen™

Ambion® Yeast tRNA purified from brewer's yeast. When used at a final concentration of 10–20 µg/mL, yeast tRNA is an effective coprecipitant to aid in recovery of small amounts of nucleic acids. It is supplied in one tube containing 0.5 mL at a concentration of 10 mg/mL. What is a Coprecipitant?

DNase I Buffer (10X) Invitrogen™

The same buffer provided with our Ambion® DNase I (AM2222, AM2224) and recombinant DNase I (AM2235) enzymes, offered here as a stand-alone product in case extra buffer is needed.

dNTPs for cDNA Probe Synthesis (10 mM) Invitrogen™

Ambion® dNTPs for cDNA Probe Synthesis (10 mM) are the same high-quality deoxynucleotide triphosphate solutions provided in the RETROscript® First Strand cDNA Synthesis Kit for RT-PCR (SKU# AM1710). They are subject to rigorous nuclease testing as well as a functional assay to ensure high levels of...

Ammonium Acetate (5 M), RNase-free Invitrogen™

Ambion® Molecular biology grade, 5 M Ammonium Acetate solution is supplied in one bottle containing 500 mL. The solution is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging.

IPTG (Dioxane-Free) Invitrogen™

Ambion® IPTG (Isopropyl-beta-D-thiogalactoside) is an inducer of the lac operon and is frequently used in conjunction with X-GAL to determine the lac phenotype of recombinant plasmids or phage. IPTG is also used to induce the expression of cloned genes that are under control of the lac operon.

MgCl2 (1 M) Invitrogen™

Molecular biology grade, Ambion® 1 M MgCl2 solution is supplied in one bottle containing 100 mL. The solution is certified RNase-free, economical, and ready-to-use. Due to the ubiquitous presence of RNases, manufacturing products for use with RNA is especially challenging.

Acid-Phenol:Chloroform, pH 4.5 (with IAA, 125:24:1) Invitrogen™

Acid Phenol:Chloroform:IAA (125:24:1) is premixed and supplied at pH 4.5 ± 0.2. Provided in one bottle of 400 mL. In RNA extraction procedures, Acid Phenol:Chloroform:IAA aids in the removal of DNA (it partitions into the organic phase), helps to stabilize the interface, and prevents foaming when...

Yeast RNA (5 mg/ml) Invitrogen™

Purified Saccharomyces cerevisiae yeast RNA is suitable as a coprecipitant in nucleic acid precipitations. It cannot be used in reactions inhibited by exogenous RNA, and it is the most inexpensive source of a high-quality coprecipitant.

Yeast RNA (10 mg/mL) Invitrogen™

Purified Torulla Ambion® yeast RNA is suitable as a coprecipitant in nucleic acid precipitations. It cannot be used in reactions inhibited by exogenous RNA, and it is the most inexpensive source of a high-quality coprecipitant.
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