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EVOS™ Vessel Holder for Automated Stage, one Nunc™ multi-well dish Invitrogen™

EVOS vessel holders allow a perfect fit of your microscope slide, cell culture flask or dish, or micro-well plate to the stage of the EVOS imaging systems for increased precision in sample alignment. This particular vessel holder securely holds one Nunc multi-well dish and is specific for the automated stage of the EVOS FL Auto Imaging System. It is just one of many easy-to-change vessel holders and stage plates available for this instrument platform. With a snug and secure fit, yet readily switched, these accessories make imaging samples in a range of vessel holders convenient and easy.

For additional choices, see the EVOS Guide to Vessel Holders and Stage Plates.
Explore the entire EVOS line of imaging systems and accessories.

Onstage Incubator for CellInsight CX7 HCA Platform Thermo Scientific™

The invitrogen Onstage Incubator for the CellInsight CX7 High Content Analysis (HCA) Platform is an environmental chamber designed specifically for the CellInsight CX7 series platforms. As an optional module, it enables precise control of temperature, humidity, and three gases for time-lapse imaging of live cells under both physiological and non-physiological conditions. Environmental settings and image acquisition parameters are all seamlessly integrated into the HCS Studio software creating a powerful, walk-away platform for live cell quantitative imaging. The Onstage Incubator for CellInsight CX7 HCA Platform can be upgraded at any time to existing compatible units.

Advantages of the Onstage Incubator for CellInsight CX7 HCA Platform:
• Fully integrated environmental chamber for live cell time-lapse imaging
• Precisely maintain physiological or non-physiological conditions
• Simple scheduling to define frequency of imaging over a time course
• Powerful kinetic and motility measurements that increase information gathered from each cell over time
• Field upgradeable
• Compatible with fixed endpoint screening using the Thermo Scientific Orbitor RS Plate Mover

Requirements
The Onstage Incubator for CellInsight CX7 HCA Platform requires the latest version of HCS Studio software and Store Image and Data Management Software.

Celleste™ 5 3D Analysis Module Invitrogen™

Celleste 5.0 Image Analysis Software is a suite of image analysis and visualization tools designed for a range of biological applications that lets you transform your images into quantitative data in a streamlined and customizable workflow. Coupled with the powerful image acquisition capabilities of our EVOS microscopes, Celleste 5 software allows you to seamlessly capture, process, measure, analyze, and share images and data. Optional modules for 2D and 3D deconvolution, visualization, and analysis allow you to customize the capabilities according to your needs. This Celleste 5 3D Analysis Module helps visualize complex 3D objects using guided segmentation designed to facilitate identification and measurements.

Celleste 5.0 software with the 3D Analysis Module offers you these important advantages:
• Streamlined workflow tools that make it easy to visualize and edit images
• Powerful image analysis capabilities for segmentation, classification, and quantification of single images or a batch of images
• Automatic segmentation of objects using any 2D frame in the Z-stack
• Seamless propagation t in Z with automated or semi-automated tools
• Automatic generation of iso-surfaces and pseudo-surfaces
• 3D counting, including co-localization
• Measurement of point-to-point distances, surfaces, and volumes

More about the deconvolution modules
Deconvolution is an algorithm-based process used to restore systematic loss of image contrast (blur) that results from the image point spread function (PSF). The powerful 2D and 3D restoration algorithms available in the Celleste 5 modules allow you to deconvolve your images to enhance contrast and quality, or to visualize them in Z, over time and by channel, then analyze the resulting image using an expanded tool set.

EVOS™ Vessel Holder for Automated Stage, with retention clip, one Nunc™ multi-well dish Invitrogen™

EVOS vessel holders allow a perfect fit of your microscope slide, cell culture flask or dish, or micro-well plate to the stage of the EVOS imaging systems for increased precision in sample alignment. This particular vessel holder securely holds one Nunc multi-well dish and is specific for the automated stage of the EVOS FL Auto Imaging System. This vessel holder also has a retention clip to more firmly hold the vessel in place. It is just one of many easy-to-change vessel holders and stage plates available for this instrument platform. With a snug and secure fit, yet readily switched, these accessories make imaging samples in a range of vessel holders convenient and easy.

For additional choices, see the EVOS Guide to Vessel Holders and Stage Plates.
Explore the entire EVOS line of imaging systems and accessories.

Celleste™ 5.0 Image Analysis Software, network server license + 1 seat Thermo Scientific™

Celleste 5.0 Image Analysis Software has features designed for a range of biological applications, such as image adjustment and processing with manual and automatic measurements over multiple channels, and segmentation and classification tools that help you transform images into quantitative data in a streamlined and customizable workflow. Optional modules for deconvolution, 3D visualization, and 3D analysis allow you to customize the capabilities according to your needs. Coupled with the powerful image acquisition capabilities of our EVOS microscopes, Celleste software allows you to seamlessly capture, process, measure, analyze, and share images and data.

This Network Server License is designed for environments where the use of multiple copies is desired. It comes with one seat; additional seats can be added at any time.

Celleste 5.0 Image Analysis Software offers you these important advantages:
• Streamlined workflow tools that make it easy to visualize and edit images
• Comprehensive image processing and enhancement functions with optional modules to meet your specific needs
• Powerful image analysis capabilities for segmentation, classification, and quantification of single images or a batch of images
• A new interface for streamlined multi-well plate navigation and well selection
• A wide range of functions to create reports and share images and data

Process and analyze
After image acquisition, immediately apply a suite of image processing and enhancement functions with a few clicks of a mouse. Use auto-alignment for overlays and remove background to reveal critical details with a wide range of filters. Once satisfied with the results from one image, apply the same steps to a set of time-lapse images, scans, or results from different treatments. When imaging cells in multi-well plates, use the new interface to select only the wells you wish to analyze and automatically process them as a set.

Measure and quantify
Easily measure and analyze your images with a variety of measurement tools, such as those for distance, region, angles, and area. The ability to identify an object in time-lapse experiments can be used to track cell movement or migration. This feature is particularly powerful in combination with the EVOS Onstage Incubator, which allows continuous monitoring of cells under controlled environmental conditions.

Count and classify
Using a number of manual and automatic measurement tools, easily count and characterize cells and subcellular objects based on area, length, shape, and light intensity. Flexible segmentation tools allow you to label images based on color or intensity level. A new ROI functionality allows you to count objects and maintain measurements by region (e.g., a parent-child application) and also to count by ROI. After object counting, sort and display counted images based on size and other parameters.

Report and share
Upon completing the image analysis, a suite of annotation and reporting tools allow you to create presentation-ready image and data reports with a few clicks and to share them with others in PDF, PowerPoint, and Excel format.

Celleste 5.0 software is a powerful image analysis solution that helps make your imaging research job easier and increase your productivity by seamlessly providing images and accurate data. With the proven usability of EVOS microscopes, you now have the tools to make it easy to capture, process, measure, analyze, and share your important images and data more effectively than ever.

Slideport™ Slide Inserts Thermo Scientific™

The Thermo Scientific™ Slideport™ Slide Insert holds microscope slides in a frame that fits Thermo Scientific™ High Content Instruments. Slides can be imaged using many objective lenses due to two available skirt heights. The Slideport Slide Insert has been tested to image a wide range of sample types including fixed and live cells, tissue sections, tissue microarrays and beads. Contents: five Slideport Slide Inserts.

Features of Slideport Slide Inserts:

• Two skirt heights available to accommodate a variety of readers and objectives
• Any microscope or plate reader that accepts standard microplates will also accept the Slideport Slide Insert
• Image a wide range of sample types including fixed and live cells, tissue sections, tissue microarrays, and beads
• Easily stack slides for automated imaging with or without lids
• Slideport Slide Insert can be utilized at a variety of temperatures from 4°to 37°C, allowing for flexibility of storage and imaging conditions

Compatible with the Thermo Scientific High Content Instruments

Celleste™ 5.0 Image Analysis Software, additional seat Thermo Scientific™

Celleste 5.0 Image Analysis Software has features designed for a range of biological applications, such as image adjustment and processing with manual and automatic measurements over multiple channels, and segmentation and classification tools that help you transform images into quantitative data in a streamlined and customizable workflow. Optional modules for deconvolution, 3D visualization, and 3D analysis allow you to customize the capabilities according to your needs. Coupled with the powerful image acquisition capabilities of our EVOS microscopes, Celleste software allows you to seamlessly capture, process, measure, analyze, and share images and data.

This Network Seat is designed for environments where the use of multiple copies is desired. It requires an active Celleste 5.0 Network Server License.

Celleste 5.0 Image Analysis Software offers you these important advantages:
• Streamlined workflow tools that make it easy to visualize and edit images
• Comprehensive image processing and enhancement functions with optional modules to meet your specific needs
• Powerful image analysis capabilities for segmentation, classification, and quantification of single images or a batch of images
• A new interface for streamlined multi-well plate navigation and well selection
• A wide range of functions to create reports and share images and data

Process and analyze
After image acquisition, immediately apply a suite of image processing and enhancement functions with a few clicks of a mouse. Use auto-alignment for overlays and remove background to reveal critical details with a wide range of filters. Once satisfied with the results from one image, apply the same steps to a set of time-lapse images, scans, or results from different treatments. When imaging cells in multi-well plates, use the new interface to select only the wells you wish to analyze and automatically process them as a set.

Measure and quantify
Easily measure and analyze your images with a variety of measurement tools, such as those for distance, region, angles, and area. The ability to identify an object in time-lapse experiments can be used to track cell movement or migration. This feature is particularly powerful in combination with the EVOS Onstage Incubator, which allows continuous monitoring of cells under controlled environmental conditions.

Count and classify
Using a number of manual and automatic measurement tools, easily count and characterize cells and subcellular objects based on area, length, shape, and light intensity. Flexible segmentation tools allow you to label images based on color or intensity level. A new ROI functionality allows you to count objects and maintain measurements by region (e.g., a parent-child application) and also to count by ROI. After object counting, sort and display counted images based on size and other parameters.

Report and share
Upon completing the image analysis, a suite of annotation and reporting tools allow you to create presentation-ready image and data reports with a few clicks and to share them with others in PDF, PowerPoint, and Excel format.

Celleste 5.0 software is a powerful image analysis solution that helps make your imaging research job easier and increase your productivity by seamlessly providing images and accurate data. With the proven usability of EVOS microscopes, you now have the tools to make it easy to capture, process, measure, analyze, and share your important images and data more effectively than ever.

InSpeck™ Green (505/515) Microscope Image Intensity Calibration Kit, 2.5 µm Invitrogen™

The InSpeck™ Green Microscopy Image Intensity Calibration Kit provides microsphere standards that generate a series of well-defined fluorescent intensity levels for constructing calibration curves and evaluating sample brightness. Each kit includes six separate suspensions of 2.5 µm fluorescent microspheres with relative fluorescence intensities of 100%, 30%, 10%, 3%, 1% and 0.3% covering the range of intensities commonly encountered in microscopy applications. Unstained control beads and mounting medium are also supplied. The aqueous suspensions of microspheres may be applied directly to the sample for calibrating fluorescence intensities or mounted separately in an adjacent well or on another slide.

EVOS™ Vessel Holder for Automated Stage, with retention, four 25 x 75 mm microscope slides Invitrogen™

EVOS vessel holders allow a perfect fit of your microscope slide, cell culture flask or dish, or micro-well plate to the stage of the EVOS imaging systems for increased precision in sample alignment. This particular vessel holder securely holds four 25 mm x 75 mm microscope slides and is specific for the automated stage of the EVOS FL Auto Imaging System. This vessel holder also has a retention clip to more firmly hold the vessel in place. It is just one of many easy-to-change vessel holders and stage plates available for this instrument platform. With a snug and secure fit, yet readily switched, these accessories make imaging samples in a range of vessel holders convenient and easy.

For additional choices, see the EVOS Guide to Vessel Holders and Stage Plates.
Explore the entire EVOS line of imaging systems and accessories.

EVOS™ Onstage Vessel Holder, one Lab-Tek coverglass chamber slide Invitrogen™

EVOS Onstage vessel holders maximize your choice of cell culture vessels when conducting time-lapse imaging experiments using the EVOS FL Auto Imaging System equipped with an EVOS Onstage Incubator. EVOS Onstage vessel holders allow a perfect fit of your micro-well plates, cell culture flasks/dishes, and slides to the master plate of the EVOS Onstage Incubator for increased precision in sample alignment. This particular vessel holder accommodates one Nunc Lab-Tek coverglass chamber slide. With a snug and secure fit, yet readily switched, these vessel holders make time-lapse imaging convenient and easy.

Learn more about the EVOS FL Auto Imaging System >

Explore the entire EVOS line of imaging systems and accessories >

Countess™ 3 Automated Cell Counter Starter Package + ABRC Extended Warranty Invitrogen™

The Countess 3 Automated Cell Counter is a benchtop cell counting system equipped with an advanced machine-learning algorithm, ruggedized optics, fully-automated focus and lighting, and image analysis software for rapid assessment of primary and immortalized cell samples

This Starter Package comes with a cell counter (Cat. No. AMQAX2000) plus:
• Reusable slide
• 500 disposable slides with trypan blue

Features of the Countess 3 Automated Cell Counter include:• Accurate—eliminates the subjectivity of manual cell counting and user-to-user variability
Fast—counts live and dead cells, measures viability, and reports average cell size with a single touch in about 20 seconds
Convenient—requires no cleaning or routine maintenance; flush-mounted high-resolution capacitive touchscreen and simple user interface provide quick startup, require minimal training, and offer automatic saving of data to USB or cloud

The Countess 3 Automated Cell Counter works in two simple steps:
1. Mix 10 µL of sample with 10 µL of trypan blue and pipet into a disposable Countess chamber slide.
2. Insert the slide into the instrument. Rapid Capture and Auto-Save modes allow cell counting with a single touch. Focus, lighting, and file saving are executed automatically.

Get accurate cell counts
The Countess 3 Automated Cell Counter uses trypan blue staining combined with auto-focus and lighting driven by a machine-learning image analysis algorithm to obtain accurate cell and viability counts. The measurement range extends from 1 × 104 to 1 × 107 cells/mL (the optimal range is 1 × 105 to 4 × 106 cells/mL), which is broader than that of a hemocytometer.

Transfer data and images to your PC or cloud
Using the USB port on the Countess 3 Automated Cell Counter and any USB drive, you can save your cell count results and images and transfer them to your PC. (For your convenience, a USB drive and WiFI dongle are supplied with the Countess 3 instrument).

ABRC Extended Warranty
Keep your cell counter up and running with an extended warranty that covers all costs for shipping, parts, and labor for repairs. Learn more about our AB Repair Center (ABRC) service plan ’

Celleste 4.0 Image Analysis Software Upgrade, single user license Invitrogen™

The Celleste 4.0 Image Analysis Software Upgrade provides all the capabilities and features of Celleste 5.0 Image Analysis Software to current users of Celleste 4 software. The most significant enhancement is compatibility with optional modules for deconvolution, 3D visualization, and 3D analysis that allow you to customize the capabilities according to your needs. Coupled with the powerful image acquisition capabilities of our EVOS microscopes, Celleste 5 software allows you to seamlessly capture, process, measure, analyze, and share images and data.

Celleste 5.0 Image Analysis Software offers you these important advantages:
• Enhanced workflow tools that make it easier than ever to generate data
• A new interface for streamlined multi-well plate navigation, well selection, and analysis
• Comprehensive image processing and enhancement functions with optional modules that meet your specific needs

Other enhancements include new ROI functionalities for the counting of objects and maintenance of measurements by region (e.g., a parent-child application) and counting by ROI, as well as an Enhanced Measured Line Editing tool that allows you to extend, merge, and split lines. And when imaging cells in multi-well plates, use the new interface to select only the wells you wish to analyze and automatically process them as a set.

InSpeck™ Blue (350/440) Microscope Image Intensity Calibration Kit, 2.5 µm Invitrogen™

The InSpeck™ Blue Microscopy Image Intensity Calibration Kit provides microsphere standards that generate a series of well-defined fluorescent intensity levels for constructing calibration curves and evaluating sample brightness. Each kit includes six separate suspensions of 2.5 µm fluorescent microspheres with relative fluorescence intensities of 100%, 30%, 10%, 3%, 1% and 0.3% covering the range of intensities commonly encountered in microscopy applications. Unstained control beads and mounting medium are also supplied. The aqueous suspensions of microspheres may be applied directly to the sample for calibrating fluorescence intensities or mounted separately in an adjacent well or on another slide.

EVOS™ Onstage Vessel Holder, two Lab-Tek or Lab-Tek-II coverglass chamber slides Invitrogen™

EVOS Onstage vessel holders maximize your choice of cell culture vessels when conducting time-lapse imaging experiments using the EVOS FL Auto Imaging System equipped with an EVOS Onstage Incubator. EVOS Onstage vessel holders allow a perfect fit of your micro-well plates, cell culture flasks/dishes, and slides to the master plate of the EVOS Onstage Incubator for increased precision in sample alignment. This particular vessel holder accommodates two Nunc Lab-Tek or Lab-Tek-II coverglass chamber slides. With a snug and secure fit, yet readily switched, these vessel holders make time-lapse imaging convenient and easy.

Learn more about the EVOS FL Auto Imaging System >

Explore the entire EVOS line of imaging systems and accessories >

X1 Upgrade for ArrayScan™ VTI HCS Reader Thermo Scientific™

The Thermo Scientific™ X1 Upgrade ArrayScan™ VTI HCS Reader takes a current Thermo Scientific ArrayScan™ VTI HCS Reader (600 and 700 series) up to the latest image capture technology with the new X1 large field of view camera and. The Photometrics X1 CCD camera boasts a 14-bit dynamic range; 2208x2208 pixel array; 4.54 micron pixel size; and 75% peak Quantum Efficiency (QE) to provide high resolution images to the Thermo Scientific™ HCS Studio™ Cell Analysis Software, and ultimately to a relational database for secure storage and access.

Features of the X1 Upgrade for ArrayScan VTI HCS Reader:

• High sensitivity—By harnessing the photon-collecting power of the Sony™ SuperHAD microlens architecture, the X1 camera becomes ultra-sensitive without sacrificing resolution (4.5µm/pixel).
• See more in every image—The cutting-edge Photometrics X1 CCD camera with the enlarged field of view and 4.54 μm pixel size.
• Hardware designed for high throughput—Processing of the data and images from the X1 camera is optimized with a powerful Windows 7, 64-bit PC with a quad core processor.
• HCS Studio™ Cell Analysis Software is the integrated instrument control, visualization and analysis software package for novice to advanced users.
• Features Thermo Scientific™ Store Express Image and Database Software using a 10GB SQL™ database

Key Technological Features:

• High-resolution, thermo-electrically cooled to 0°C; 14-bit dynamic range; 2208x2208 pixel array; 4.54 micron pixel size; 75% peak Quantum Efficiency (QE); USB 2.0 (no additional CameraLink or FireWire Cards needed); 1x1, 2x2 or 4x4 binning options
• Binning option applied before digitization, which reduces read noise and provides for a 4:1 signal-to-noise ratio
• Larger imaging chip (2208 x 2208 px.)
• Sony SuperHAD microlens architecture for light collection
• Windows 7 Professional 64Bit Cube Computer with minimum quad core 3.7 GHz processor, 32GB RAM, and 500GB Hard Drive space for local images and data for instrument
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