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Genomics and Proteomics


CaptureSelect™ KappaXP EvolveD™ Column Thermo Scientific™

CaptureSelect KappaXP EvolveD columns are ready-to-use columns designed specifically for use in biopharmaceutical applications. These pre-packed columns are suitable for cGMP manufacturing and have the following features:
• Prepared and packed in a classified ISO7 environment
• An entirely non-metallic flow path
• Qualified in terms of performance and pressure flow characteristics
• Suitable for direct connection to standard chromatography systems
• Available Regulatory Support File
• Packed with CaptureSelect KappaXP Affinity Matrix, offering high dynamic binding capacity and high resolution over a wide range of flow rates

Please note that CaptureSelect EvolveD affinity columns are made to order. They are custom manufactured two-at-a-time, so are offered only in quantities that are a multiple of two. For detailed information on the resin, please see the CaptureSelect EvolveD column manual below.

Operating and technical specifications
Column type10-cm bed height20-cm bed height
Column diameter (cm)7102071020
Column length (cm)101010202020
Cross-sectional area (cm2)38.578.5314.238.578.5314.2
Column volume (mL)385785314277015706284

• Pressure rating (bar/psi): 4/58
• Operating temperature: 2–30°C, dependent on the resin packed
• Bed support size: 8 µm
• Inlet/outlet internal diameter: 3 mm or 6 mm depending on the column diameter
• Inlet/outlet port connections: 25-mm TC sanitary fitting

View our comprehensive column overview › (Select the Antibody Purification tab.)

CaptureSelect KappaXP Affinity Matrix recognizes the kappa light-chain of human antibodies, enabling purification of all Kappa containing Ig’s, Fab, and Fab2 fragments directly from complex sources and feed streams.

Features of this affinity matrix include:
• Purification of Kappa monoclonal antibodies, Fab-, and Fab2 fragments directly from complex source materials in a single step with high purity and yield
• Mild elution for Fab fragments and antibodies
• Human specific, no binding to bovine antibodies
• Excellent pH stability up to 100 mM NaOH for limited exposure
• Non-animal derived

View other antibody affinity matrices ›

Free of animal components
CaptureSelect products are affinity ligands created with proprietary technology based on camelid-derived single-domain antibody fragments. The KappaXP ligand is a 13-kDa fragment comprising the three complementarity determining regions (CDRs) that form the antigen binding domain. The ligand is efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.

Main characteristics:

Matrix: agarose-based, epoxide-activated
Average particle size: 65 ± 10 µm
Ligand: CaptureSelect KappaXP affinity ligand
Ligand coupling method: aldehyde coupling via NH2 residues of the ligand
Binding capacity: 20–30 g IgG Kappa-Fab/liter resin depending on flow rate, column height, and contact time
Elution conditions: 20 mM citric or acetic acid, pH 3–4; 100 mM Tris, 1.5 M MgCl2 pH 6
Max. operation pressure: 2 bar
Formulation buffer: 20% (v/v) ethanol

CaptureSelect™ tPA EvolveD™ Column Thermo Scientific™

CaptureSelect tPA EvolveD columns are ready-to-use columns designed specifically for use in biopharmaceutical applications. These pre-packed columns are suitable for cGMP manufacturing and have the following features:
• Prepared and packed in a classified ISO7 environment
• An entirely non-metallic flow path
• Qualified in terms of performance and pressure flow characteristics
• Suitable for direct connection to standard chromatography systems
• Available Regulatory Support File
• Packed with CaptureSelect tPA Affinity Matrix, offering high dynamic binding capacity and high resolution over a wide range of flow rates

Please note that CaptureSelect EvolveD affinity columns are made to order. They are custom manufactured two-at-a-time, so are offered only in quantities that are a multiple of two. For detailed information on the resin, please see the CaptureSelect EvolveD column manual below.

Operating and technical specifications
Column type10-cm bed height20-cm bed height
Column diameter (cm)7102071020
Column length (cm)101010202020
Cross-sectional area (cm2)38.578.5314.238.578.5314.2
Column volume (mL)385785314277015706284

• Pressure rating (bar/psi): 4/58
• Operating temperature: 2–30°C, dependent on the resin packed
• Bed support size: 8 µm
• Inlet/outlet internal diameter: 3 mm or 6 mm depending on the column diameter
• Inlet/outlet port connections: 25-mm TC sanitary fitting

View our comprehensive column overview › (Select the Antibody Purification tab.)

CaptureSelect tPA Affinity Matrix is designed for the purification of human tissue plasminogen activator from recombinant sources.

Features of this affinity matrix include:
• Selective for human tPA
• Enables efficient purification of recombinant tPA with high yields and purity
• Excellent pH stability up to 50 mM NaOH for limited exposure
• Non-animal derived

View other biosimilars and recombinant proteins affinity matrices ›

Free of animal components
CaptureSelect products are affinity ligands created with proprietary technology based on camelid-derived single-domain antibody fragments. The tPA ligand is a 13-kDa fragment comprising the three complementarity determining regions (CDRs) that form the antigen binding domain. The ligand is efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.

Main characteristics:

Matrix: agarose-based, epoxide-activated
Average particle size: 65 ± 10 µm
Ligand: CaptureSelect tPA affinity ligand
Ligand coupling method: aldehyde coupling via NH2 residues of the ligand
Binding capacity: 8–10 mg tPA/mL resin depending on flow rate, column height, and contact time
Neutral elution conditions: 20 mM Tris, 2.0 M MgCl2, 0.2 M arginine, pH 7.0
Acidic elution conditions: 0.1 M glycine, pH 3.0
Max. operation pressure: 2 bar
Formulation buffer: 20% (v/v) ethanol

HisPur™ Ni-NTA Spin Columns, 0.2 mL Thermo Scientific™

Thermo Scientific HisPur Ni-NTA Spin Columns contain a high-capacity, high-performance nickel-IMAC resin for routine affinity purification of His-tagged fusion proteins.

Features of HisPur Ni-NTA Resin:

High capacity—bind up to 60 mg of 6xHis-tagged protein per milliliter of resin
Versatile—purify proteins using native or denaturing conditions
Compatible—use with Thermo Scientific Cell Lysis Reagents and a variety of buffer additives
Cost-effective—reuse the same batch of resin at least five times
Easy to use—pre-formulated buffers available for kit formats
Flexible—available in multiple formats including bulk resin, spin columns, chromatography cartridges and 96-well filter plates

The specially prepared support consists of beaded agarose derivatized with the nitrilotriacetic acid (NTA) chelation moiety and loaded with divalent nickel ions (Ni2+). The immobilized metal affinity chromatography (IMAC) resin provides exceptional binding capacity and performance for recombinant His-tagged protein purification.

The expression and purification of recombinant proteins is central to protein regulation, structure and function studies. The majority of recombinant proteins are expressed as fusions with short affinity tags, the most popular being the polyhistidine (6xHis) tag. The method used to purify recombinant His-tagged proteins is immobilized metal affinity chromatography (IMAC), consisting of chelating resins charged with either nickel or cobalt ions that coordinate with the histidine side chains.

HisPur Ni-NTA Resin effectively purifies high levels of overexpressed His-tagged fusion proteins from bacterial lysates, such as those that are attained with Thermo Scientific B-PER Bacterial Protein Extraction Reagents. The resin performs well in batch-binding and spin-column procedures at a variety of scales. Performance equals or exceeds popular Ni-NTA resins from other suppliers. HisPur Ni-NTA Resin is a high-quality, stable and resilient affinity support. Tests confirm that no decrease in performance occurs after at least five repeated uses. These data indicated that the resin is highly resistant to structural degradation or nickel ion leaching during normal use.

HisPur Ni-NTA Resin and HisPur Cobalt Resin are alternative forms of IMAC, the former using nickel and the latter using cobalt as the chelated metal ion responsible for His-tag binding. Ni-NTA resins have been the most common IMAC resin choice for 6xHis-tag protein purifications because of the four metal-binding sites on the chelate, which enables high-protein binding and low-metal ion leaching. Cobalt binds less strongly and therefore more discriminantly, enabling greater purity but usually less yield in His-tagged protein purification.

Available Formats:
Resin Slurries—crosslinked 6% beaded agarose; 10 mL, 100 mL, 500 mL bottles
Spin Columns—0.2 mL (microcentrifuge), 1 mL, 3 mL columns
Purification Kits—complete kits in all three column sizes
Chromatography Cartridges—learn more and see HisPur Chromatography Cartridge data
96-well Spin Plates

Related Products
HisPur™ Ni-NTA Resin
HisPur™ Ni-NTA Spin Purification Kit, 0.2 mL
HisPur™ Ni-NTA Spin Plates

Triton™ X-114 Surfact-Amps™ Detergent Solution Thermo Scientific™

Thermo Scientific Triton X-114 Surfact-Amps Detergent Solution is a stabilized 10% (w/v) solution of highly-purified Triton™ X-114 detergent, supplied in glass ampules and packaged under nitrogen inert gas, ensuring their stability and eliminating the accumulation of peroxides and degradation products.

Features of Triton X-114 Surfact-Amps Detergent Solution:

Triton X-114—a nonionic detergent for use in various routine protein and molecular biology techniques
Accurate—precise 10% detergent solution in ultrapure water
Easy-to-use—solution is simple to dispense and dilute for use
Exceptionally pure—less than 1.0µeq/mL peroxides and carbonyls
Stable— packaged in glass ampules under inert nitrogen gas

Specifications for Triton X-114 Surfact-Amps Detergent Solution:
• Visual: Slightly hazy to cloudy, colorless solution, free of particulates; may form layers.
• Concentration: 10.0±1.0%
• Oxidants: ≤1.0µeq/mL
• Carbonyls: ≤1.0µeq/mL
• Suspended Solids: Residue present must not exceed Residue Reference.

Properties of Triton X-114:
• Molecular Weight: 537g
• Detergent Class: Nonionic polyoxyethylene surfactant
• Aggregation Number: unknown
• Micelle Molecular Weight: unknown
• Critical Micelle Concentration (CMC): 0.21 mM (0.0113%, w/v)
• Cloud Point: 23°C
• Dialyzable: No

Triton X-114 is most frequently used as a component of cell lysis buffers. In certain conditions, this detergent phase-separates in aqueous solutions, a property that has been used to separate hydrophilic and hydrophobic (membrane) proteins.

For added safety when opening glass ampules, consider using our Ampule Breakers, which are disposable safety devices that protect the fingers when breaking open a glass ampule.

Related Products
Surfact-Amps™ Detergent Sampler

MembranePro™ Functional Protein Support Kit Invitrogen™

For customers studying GPCRs and other membrane proteins, MembranePro™ is the ready-to-use system that delivers enriched, functional membrane proteins efficiently and reliably. Benefits of MembranePro™ include:

• Proteins are displayed on human (or other mammalian) cell membranes
• Cellular quality control and mammalian posttranslational processing helps produce functional protein
• Proteins bud off as lipoparticles and are easily collected from the culture medium
• Lipoparticles are enriched with expressed receptors

MembranePro™ workflow is less labor intensive in comparison to traditional production of membrane fractions. Briefly, the MembranePro™ protocol allows you to:

1. Clone your gene of interest using a TOPO® vector
2. Transfect with Lipofectamine™ 2000 and MembranePro™ Reagent into 293FT cells
3. Harvest lipoparticles 48h post-transfection, concentrate with MembranePro™ Precipitation Mix
4. Store lipoparticles at -80 C or proceed to downstream assays

MembranePro™ products are offered in two configurations – as an expression kit (TOPO® vector, Lipofectamine™ 2000, MembranePro™ Precipitation Mix, MembranePro™ Reagent, 293FT cells) and as a support kit (Lipofectamine™ 2000, MembranePro™ Precipitation Mix, MembranePro™ Reagent). The expression kit is only available in a 10 reaction size; the support kit is available in 10, 60 and 600 reaction sizes.

HisPur™ Ni-NTA Spin Columns, 1 mL Thermo Scientific™

Thermo Scientific HisPur Ni-NTA Spin Columns contain a high-capacity, high-performance nickel-IMAC resin for routine affinity purification of His-tagged fusion proteins.

Features of HisPur Ni-NTA Resin:

High capacity—bind up to 60 mg of 6xHis-tagged protein per milliliter of resin
Versatile—purify proteins using native or denaturing conditions
Compatible—use with Thermo Scientific Cell Lysis Reagents and a variety of buffer additives
Cost-effective—reuse the same batch of resin at least five times
Easy to use—pre-formulated buffers available for kit formats
Flexible—available in multiple formats including bulk resin, spin columns, chromatography cartridges and 96-well filter plates

The specially prepared support consists of beaded agarose derivatized with the nitrilotriacetic acid (NTA) chelation moiety and loaded with divalent nickel ions (Ni2+). The immobilized metal affinity chromatography (IMAC) resin provides exceptional binding capacity and performance for recombinant His-tagged protein purification.

The expression and purification of recombinant proteins is central to protein regulation, structure and function studies. The majority of recombinant proteins are expressed as fusions with short affinity tags, the most popular being the polyhistidine (6xHis) tag. The method used to purify recombinant His-tagged proteins is immobilized metal affinity chromatography (IMAC), consisting of chelating resins charged with either nickel or cobalt ions that coordinate with the histidine side chains.

HisPur Ni-NTA Resin effectively purifies high levels of overexpressed His-tagged fusion proteins from bacterial lysates, such as those that are attained with Thermo Scientific B-PER Bacterial Protein Extraction Reagents. The resin performs well in batch-binding and spin-column procedures at a variety of scales. Performance equals or exceeds popular Ni-NTA resins from other suppliers. HisPur Ni-NTA Resin is a high-quality, stable and resilient affinity support. Tests confirm that no decrease in performance occurs after at least five repeated uses. These data indicated that the resin is highly resistant to structural degradation or nickel ion leaching during normal use.

HisPur Ni-NTA Resin and HisPur Cobalt Resin are alternative forms of IMAC, the former using nickel and the latter using cobalt as the chelated metal ion responsible for His-tag binding. Ni-NTA resins have been the most common IMAC resin choice for 6xHis-tag protein purifications because of the four metal-binding sites on the chelate, which enables high-protein binding and low-metal ion leaching. Cobalt binds less strongly and therefore more discriminantly, enabling greater purity but usually less yield in His-tagged protein purification.

Available Formats:
Resin Slurries—crosslinked 6% beaded agarose; 10 mL, 100 mL, 500 mL bottles
Spin Columns—0.2 mL (microcentrifuge), 1 mL, 3 mL columns
Purification Kits—complete kits in all three column sizes
Chromatography Cartridges—learn more and see HisPur Chromatography Cartridge data
96-well Spin Plates

Related Products
HisPur™ Ni-NTA Resin
HisPur™ Ni-NTA Spin Purification Kit, 1 mL
HisPur™ Ni-NTA Chromatography Cartridges, 1 mL

CaptureSelect™ TSH EvolveD™ Column Thermo Scientific™

CaptureSelect TSH EvolveD columns are ready-to-use columns designed specifically for use in biopharmaceutical applications. These pre-packed columns are suitable for cGMP manufacturing and have the following features:
• Prepared and packed in a classified ISO7 environment
• An entirely non-metallic flow path
• Qualified in terms of performance and pressure flow characteristics
• Suitable for direct connection to standard chromatography systems
• Available Regulatory Support File
• Packed with CaptureSelect TSH Affinity Matrix, offering high dynamic binding capacity and high resolution over a wide range of flow rates

Please note that CaptureSelect EvolveD affinity columns are made to order. They are custom manufactured two-at-a-time, so are offered only in quantities that are a multiple of two. For detailed information on the resin, please see the CaptureSelect EvolveD column manual below.

Operating and technical specifications
Column type10-cm bed height20-cm bed height
Column diameter (cm)7102071020
Column length (cm)101010202020
Cross-sectional area (cm2)38.578.5314.238.578.5314.2
Column volume (mL)385785314277015706284

• Pressure rating (bar/psi): 4/58
• Operating temperature: 2–30°C, dependent on the resin packed
• Bed support size: 8 µm
• Inlet/outlet internal diameter: 3 mm or 6 mm depending on the column diameter
• Inlet/outlet port connections: 25-mm TC sanitary fitting

View our comprehensive column overview › (Select the Antibody Purification tab.)

CaptureSelect TSH Affinity Matrix is designed for the purification of thyroid-stimulating hormone (TSH) from recombinant sources.

Features of this affinity matrix include:
• Highly selective for TSH with no affinity for the other gonadotropins (FSH, LH, and HCG)
• Enables efficient purification of recombinant TSH with high yields and retained activity
• Human specific
• Non-animal derived

View other biosimilars and recombinant proteins affinity matrices ›

Free of animal components
CaptureSelect products are affinity ligands created with proprietary technology based on camelid-derived single-domain antibody fragments. The TSH ligand is a 14-kDa fragment comprising the three complementarity determining regions (CDRs) that form the antigen binding domain. The ligand is efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.

Main characteristics:

Matrix: agarose-based, epoxide-activated
Average particle size: 65 ± 10 µm
Ligand: CaptureSelect TSH affinity ligand
Ligand coupling method: epoxide coupling
Binding capacity: ~5 mg TSH/mL resin depending on flow rate, column height, and contact time
Elution conditions: 100 mM citrate buffer, pH 3.0
Max. operation pressure: 2 bar
Formulation buffer: 20% (v/v) ethanol

CaptureSelect™ FcXL EvolveD™ Column Thermo Scientific™

CaptureSelect FcXL EvolveD columns are ready-to-use columns designed specifically for use in biopharmaceutical applications. These pre-packed columns are suitable for cGMP manufacturing and have the following features:
• Prepared and packed in a classified ISO7 environment
• An entirely non-metallic flow path
• Qualified in terms of performance and pressure flow characteristics
• Suitable for direct connection to standard chromatography systems
• Available Regulatory Support File
• Packed with CaptureSelect FcXL Affinity Matrix, offering high dynamic binding capacity and high resolution over a wide range of flow rates

Please note that CaptureSelect EvolveD affinity columns are made to order. They are custom manufactured two-at-a-time, so are offered only in quantities that are a multiple of two. For detailed information on the resin, please see the CaptureSelect EvolveD column manual below.

Operating and technical specifications
Column type10-cm bed height20-cm bed height
Column diameter (cm)7102071020
Column length (cm)101010202020
Cross-sectional area (cm2)38.578.5314.238.578.5314.2
Column volume (mL)385785314277015706284

• Pressure rating (bar/psi): 4/58
• Operating temperature: 2–30°C, dependent on the resin packed
• Bed support size: 8 µm
• Inlet/outlet internal diameter: 3 mm or 6 mm depending on the column diameter
• Inlet/outlet port connections: 25-mm TC sanitary fitting

View our comprehensive column overview › (Select the Antibody Purification tab.)

CaptureSelect FcXL Affinity Matrix is designed for the purification of recombinant human IgG, Fc fusion proteins, and plasma derived IgG. It recognizes the CH3 domain of all human IgG subclasses (IgG1, 2, 3, and 4).

Features of this affinity matrix include:
• Purification of IgGs and Fc fusion proteins directly from complex source materials in a single step with high purity and yield
• Mild elution making it suitable for Fc fusion proteins
• Human specific, no binding to bovine antibodies
• Non-animal derived

View other antibody affinity matrices ›

Free of animal components
CaptureSelect products are affinity ligands created with proprietary technology based on camelid-derived single-domain antibody fragments. The FcXL ligand is a 13-kDa fragment comprising the three complementarity determining regions (CDRs) that form the antigen binding domain. The ligand is efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.

Main characteristics:

Matrix: agarose-based, epoxide-activated
Average particle size: 65 ± 10 µm
Ligand: CaptureSelect FcXL affinity ligand
Ligand coupling method: aldehyde coupling via NH2 residues of the ligand
Binding capacity: 25–35 g IgG/L resin depending on flow rate, column height, and contact time
Elution conditions: 20 mM citric or acetic acid, pH 3–4, or 20 mM sodium acetate, 1.0 M MgCl2, 40% (v/v) propylene glycol, pH 5–6
Max. operation pressure: 2 bar
Formulation buffer: 20% (v/v) ethanol

Pierce™ Centrifuge Columns, 2 mL Thermo Scientific™

Thermo Scientific Pierce Centrifuge Columns are convenient tools for handling 40 µL to 10 mL of an affinity support for purification.

Column details:

• Made from low protein binding polypropylene for compatibility with protein purification
• Total volume: 5 mL (2 mL resin bed, 3 mL reservoir)
• Resin volume: 2 mL
• Filter type, pore size: Polyethylene filter, ~30 µm pore size
• Receiver tube: Fits standard 15 mL conical centrifuge tubes
• Caps: Screw-top cap
• Twist-off bottom and press-on cap to reseal

Simply add the affinity resin to one of the polypropylene columns, remove the twist-off bottom and allow the resin to pack itself. Then add your sample and allow it to bind to the support and use a centrifuge to efficiently wash away any contaminants and elute your purified protein. Pierce Centrifuge Columns can be used in either spin or gravity-flow format. The spin format reduces sample processing time and makes column washing and elution more efficient. For gravity-flow procedures and when slow processing time is desired for binding steps with dilute samples, Column Extenders can be used to increase the column reservoir capacity. Use the any combination of processing techniques to get the best possible chromatography results.

Applications:

• Affinity purification, affinity chromatography
• Immunodepletion
• Ion exchange resins
• Gel filtration, desalting, buffer exchange

Related Products
Pierce™ Centrifuge Columns, 0.8 mL
Pierce™ Centrifuge Columns, 5 mL
Pierce™ Centrifuge Columns, 10 mL
Column Extenders for Pierce™ Centrifuge Columns

CaptureSelect™ HRP Anti-AAV9 Conjugate Thermo Scientific™

CaptureSelect HRP Anti-AAV9 Conjugate includes a 13-kDa camelid antibody fragment that specifically binds adeno-associated virus particles of serotype 9 (AAV9). The antibody fragment is chemically conjugated to horseradish peroxidase (HRP) via a spacer of appropriate length to maintain full binding capacity of the antibody fragment.

CaptureSelect HRP Anti-AAV9 Conjugate can be combined with CaptureSelect Biotin Anti-AAV9 Conjugate (Cat. No. 7103332100) in enzyme-linked immunoassays to quantify the total number of AAV9 capsids. Sandwich ELISA set-ups can be designed using the biotin anti-AAV9 conjugate as a capture reagent immobilized on a streptavidin coated surface. HRP anti-AAV9 conjugate is then used for detection of bound AAV9 capsids.

For assay development to measure total capsids for other AAV serotypes, we recommend CaptureSelect Biotin Anti-AAVX Conjugate (Cat. No. 7103522100) and CaptureSelect HRP Anti-AAVX Conjugate (Cat. No. 7303522100).

Product details

Format type:HRP, liquid
Buffer: PBS, 50% (v/v) glycerol
Storage: in freezer (-5 to -30°C)
Concentration: 0.5 mg/mL
Volume: 0.1 mL
Recommended working dilution for sandwich ELISA: 1: 5,000 to 1: 10,000 (the optimal working dilution should be determined empirically for each application)

Pierce™ 3x DYKDDDDK Peptide Thermo Scientific™

Pierce 3x DYKDDDDK Peptide is a lyophilized peptide with the sequence Met-Asp-Tyr-Lys-Asp-His-Asp-Gly-Asp-Tyr-Lys-Asp-His-Asp-Ile-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys (MDYKDHDGDYKDHDIDYKDDDDK). It has a molecular weight of 2861.9 g/mol. For use, reconstitute to 1.5 mg/mL in PBS.

This tricosa-peptide can be used to competitively elute DYKDDDDK-tagged fusion proteins from immobilized anti-DYKDDDDK affinity supports, especially when a DYKDDDDK-tagged protein is sensitive to low pH. Note that Pierce 3x DYKDDDDK Peptide may interfere with protein determination assays and absorbance at 280 nm. However, the peptide can be removed from the eluate by desalting (e.g., with Zeba Spin Desalting Columns, Cat. No. 89891).

POROS™ CaptureSelect™ AAVX Affinity Resin Thermo Scientific™

POROS CaptureSelect AAVX Affinity Resin is engineered to address the high selectivity and capacity requirements for large-scale downstream purification of a broad range of naturally occurring and synthetic adeno-associated virus (AAV) serotypes used for gene therapy applications. POROS CaptureSelect AAVX resin has demonstrated binding reactivity towards a set of AAV serotypes that includes AAV1 to AAV8, and AAVrh10 as well as synthetic serotypes.

Features of POROS CaptureSelect AAVX resin include:
• One-step AAV purification from crude material with high purity and yield
• High specificity and capacity, maximizing yield by significantly reducing process steps
• Superior capacity maintained at high flow rates
• Excellent scalability and easier handling and packing due to rigid polymeric bead
• Non-animal derived

POROS CaptureSelect AAVX Affinity Resin enables a robust, fast, and platformable purification process for wide-ranging AAV serotypes with excellent purity and yield obtained in one step. This resin comes with complete technical and regulatory support.

View other viral vector resins and supporting products ›

Main characteristics

Matrix: cross-linked poly(styrene-divinylbenzene) POROS beads
Average particle size: 50 µm
Ligand: CaptureSelect AAVX affinity ligand
Binding capacity: >1x1013 genome copies/mL of resin
Elution conditions: acidic elution with 0.1 M glycine, pH 3.0
Mechanical resistance: 100 bar (1,450 psi; 10 MPa)
Formulation buffer: 18% (v/v) ethanol

CaptureSelect affinity ligands are created using a proprietary technology based on camelid-derived single-domain antibody fragments. The ligand is a 13-kDa fragment that comprises the three complementarity determining regions (CDRs) that form the antigen-binding domain, efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.

HisPur™ Ni-NTA Spin Columns, 3 mL Thermo Scientific™

Thermo Scientific HisPur Ni-NTA Spin Columns contain a high-capacity, high-performance nickel-IMAC resin for routine affinity purification of His-tagged fusion proteins.

Features of HisPur Ni-NTA Resin:

High capacity—bind up to 60 mg of 6xHis-tagged protein per milliliter of resin
Versatile—purify proteins using native or denaturing conditions
Compatible—use with Thermo Scientific Cell Lysis Reagents and a variety of buffer additives
Cost-effective—reuse the same batch of resin at least five times
Easy to use—pre-formulated buffers available for kit formats
Flexible—available in multiple formats including bulk resin, spin columns, chromatography cartridges and 96-well filter plates

The specially prepared support consists of beaded agarose derivatized with the nitrilotriacetic acid (NTA) chelation moiety and loaded with divalent nickel ions (Ni2+). The immobilized metal affinity chromatography (IMAC) resin provides exceptional binding capacity and performance for recombinant His-tagged protein purification.

The expression and purification of recombinant proteins is central to protein regulation, structure and function studies. The majority of recombinant proteins are expressed as fusions with short affinity tags, the most popular being the polyhistidine (6xHis) tag. The method used to purify recombinant His-tagged proteins is immobilized metal affinity chromatography (IMAC), consisting of chelating resins charged with either nickel or cobalt ions that coordinate with the histidine side chains.

HisPur Ni-NTA Resin effectively purifies high levels of overexpressed His-tagged fusion proteins from bacterial lysates, such as those that are attained with Thermo Scientific B-PER Bacterial Protein Extraction Reagents. The resin performs well in batch-binding and spin-column procedures at a variety of scales. Performance equals or exceeds popular Ni-NTA resins from other suppliers. HisPur Ni-NTA Resin is a high-quality, stable and resilient affinity support. Tests confirm that no decrease in performance occurs after at least five repeated uses. These data indicated that the resin is highly resistant to structural degradation or nickel ion leaching during normal use.

HisPur Ni-NTA Resin and HisPur Cobalt Resin are alternative forms of IMAC, the former using nickel and the latter using cobalt as the chelated metal ion responsible for His-tag binding. Ni-NTA resins have been the most common IMAC resin choice for 6xHis-tag protein purifications because of the four metal-binding sites on the chelate, which enables high-protein binding and low-metal ion leaching. Cobalt binds less strongly and therefore more discriminantly, enabling greater purity but usually less yield in His-tagged protein purification.

Available Formats:
Resin Slurries—crosslinked 6% beaded agarose; 10 mL, 100 mL, 500 mL bottles
Spin Columns—0.2 mL (microcentrifuge), 1 mL, 3 mL columns
Purification Kits—complete kits in all three column sizes
Chromatography Cartridges—learn more and see HisPur Chromatography Cartridge data
96-well Spin Plates

Related Products
HisPur™ Ni-NTA Resin
HisPur™ Ni-NTA Spin Purification Kit, 3 mL
HisPur™ Ni-NTA Chromatography Cartridges, 5 mL

SureQuant™ AKT Pathway Relative Quantitation Module Thermo Scientific™

The Thermo Scientific SureQuant AKT Pathway Relative Quantitation Module provides a complete quantitation solution for the analysis of key AKT signaling pathway proteins using mass spectrometry (MS).

SureQuant AKT Pathway Relative Quantitation Module features include:
• Complete—contains validated AQUA HeavyPeptide mix, diluent, matrix, system suitability standard including optimized acquisition and analysis methods
• Validated—peptides rigorously tested for successful quantitation of each target peptide
• Multiplex—able to quantitate AKT, PTEN, IRS1, IGF1-R, GSK3a, GSK3b, PRAS40, mTOR, p70S6K, and TSC2 in a single reaction

The AKT signaling pathway serves as a master regulatory pathway for cell growth and proliferation. Aberrant activation of this pathway promotes survival of tumor cells in many types of cancer. This multiplex kit is ideal for monitoring protein expression of ten key proteins in the AKT signaling pathway (AKT, PTEN, IRS1, IGF1-R, GSK3a, GSK3b, PRAS40, mTOR, p70S6K, and TSC2) in a single assay. The ability to simultaneously monitor and quantitate these targets in a single sample is a powerful tool for assessment of normal and abnormal cell growth.

The SureQuant AKT Pathway Relative Quantitation Module provides reagents for relative quantitation of 30 unique peptides from 10 AKT-mTOR signaling pathway target proteins using liquid chromatography (LC) and MS. AQUA Ultimate HeavyPeptide mixture is provided for use as a spike-in internal standard for relative quantitation in the multiplexed sample. Standardized methods and data process templates are provided for streamlined data acquisition and analysis.

The Pierce LC-MS/MS System Suitability Standard (7 x 5 Mix) is included in this module to assess dynamic range of LC-MS/MS systems before running the calibration curves and samples.

This kit can be used with the SureQuant AKT Pathway IP and MS Sample Preparation Module for enriched multiplex sample preparation. When used together, these modules provide a complete solution for sample preparation and analysis of the target proteins in the pathway.

Related products:
EASY-Spray LC Column
SureQuant AKT Pathway (Phospho) Multiplex Panel (Absolute Quantitation)
Pierce Trypsin Protease, MS Grade
Pierce LC-MS/MS System Suitability Standard

CaptureSelect™ CH1-XL EvolveD™ Column Thermo Scientific™

CaptureSelect CH1-XL EvolveD columns are ready-to-use columns designed specifically for use in biopharmaceutical applications. These pre-packed columns are suitable for cGMP manufacturing and have the following features:
• Prepared and packed in a classified ISO7 environment
• An entirely non-metallic flow path
• Qualified in terms of performance and pressure flow characteristics
• Suitable for direct connection to standard chromatography systems
• Available Regulatory Support File
• Packed with CaptureSelect CH1-XL Affinity Matrix, offering high dynamic binding capacity and high resolution over a wide range of flow rates

Please note that CaptureSelect EvolveD affinity columns are made to order. They are custom manufactured two-at-a-time, so are offered only in quantities that are a multiple of two. For detailed information on the resin, please see the CaptureSelect EvolveD column manual below.

Operating and technical specifications
Column type10-cm bed height20-cm bed height
Column diameter (cm)7102071020
Column length (cm)101010202020
Cross-sectional area (cm2)38.578.5314.238.578.5314.2
Column volume (mL)385785314277015706284

• Pressure rating (bar/psi): 4/58
• Operating temperature: 2–30°C, dependent on the resin packed
• Bed support size: 8 µm
• Inlet/outlet internal diameter: 3 mm or 6 mm depending on the column diameter
• Inlet/outlet port connections: 25-mm TC sanitary fitting

View our comprehensive column overview › (Select the Antibody Purification tab.)

CaptureSelect CH1-XL Affinity Matrix recognizes the CH1 domain of human IgG antibodies, enabling purification of all subclasses (1, 2, 3, and 4) of human IgG and Fab fragments, independent of the light-chain isotype and source material.

Features of this affinity matrix include:
• Platform approach for purification of IgGs and their Fab fragments directly from complex source materials in a single step with high purity and yield
• Elution possible at pH 4.0–4.5 with 50 mM acetate buffer
• No co-purification of overproduced free light chain due to selectivity for the CH1 domain
• No binding to bovine antibodies, so presence of fetal bovine serum is not a problem
• Excellent pH stability up to 50 mM NaOH for limited exposure
• Non-animal derived

View other antibody affinity matrices ›

Free of animal components
CaptureSelect products are affinity ligands created with proprietary technology based on camelid-derived single-domain antibody fragments. The CH1-XL ligand is a 13-kDa fragment comprising the three complementarity determining regions (CDRs) that form the antigen binding domain. The ligand is efficiently produced by the yeast Saccharomyces cerevisiae in a production process free of animal components.

Main characteristics:

Matrix: agarose-based, epoxide-activated
Average particle size: 65 ± 10 µm
Ligand: CaptureSelect CH1-XL affinity ligand
Ligand coupling method: epoxide coupling
Binding capacity: >15 mg/mL human Fab fragment depending on flow rate, column height, contact time. For humanized Fabs, lower capacities can be observed.
Elution conditions: 100 mM glycine buffer, pH 3.0; 50 mM acetate buffer, pH 4.0–4.5
Max. operation pressure: 2 bar
Formulation buffer: 20% (v/v) ethanol

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