Shop All Secondary Detection Kits & Reagents

TRA-1-60 Alexa Fluor™ 488 Conjugate Kit for Live Cell Imaging (Invitrogen™)

Molecular Probes® human pluripotent stem cell (PSC) live imaging kits provide reagents for superior imaging of live human PSCs. This TRA-1-60 Alexa Fluor® 488 Conjugate Kit includes an optimally labeled primary antibody paired with reduced-background imaging medium (FluoroBrite™ DMEM) for the specific detection of the PSC marker TRA-1-60. For fixed cell staining of PSC markers and germ layer markers, we recommend the Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit.

With the TRA-1-60 Alexa Fluor® 488 Conjugate Kit for Live Cell Imaging, you can:
• Confirm the expression of a key pluripotent stem cell marker using an optimally dye-conjugated antibody
• Preserve cell health and maximize fluorescence signal with FluoroBrite™ DMEM
• Stain live cell samples with confidence using reagents that are tested to be free of common contaminants

Confirm Expression of a Key PSC Marker
Powered by the high performance of bright, photostable Alexa Fluor® dyes, this kit enables reliable live-cell detection of the surface PSC marker TRA-1-60. This antibody conjugate has been demonstrated to show superior staining results when used with FluoroBrite™ DMEM.

Preserve Cell Health and Maximize Signal
Staining cells in FluoroBrite™ DMEM maximizes cell health and enhances signal-to-noise ratio when performing live-cell fluorescence imaging.

Stain Live-cell Samples with Confidence
Unlike most other conjugated antibodies, the reagents provided in this kit are 0.2 µm sterile-filtered and tested to be free of common contaminants.

What You Get
Easily scalable to any culture plate, dish, or slide format, the TRA-1-60 Alexa Fluor® 488 Conjugate Kit comes with reagents sufficient to stain 50 samples using a 200 µL staining volume and includes:
• 200 µL of Alexa Fluor® 488 Mouse Anti-Human TRA-1-60 (50X concentration)
• 500 mL FluoroBrite™ DMEM

Alexa Fluor™ 568 Tyramide Reagent (Invitrogen™)

Alexa Fluor™ 568 Tyramide Reagent is a component of SuperBoost™ tyramide signal amplification kits made available separately here. This reagent can be used with any antibody conjugated to HRP for tyramide signal amplification for detection of low abundance targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH).

SuperBoost reagents, when used as directed, can increase sensitivity up to 200 times over standard imaging methods. For standout research, SuperBoost reagents sharpen your results for clear visibility into critical areas that standard imaging methods fail to reveal. SuperBoost reagents are simple to use and easily adapted to standard ICC, IHC, or FISH experimental protocols, using any cell or tissue type. Cells labeled using a SuperBoost reagent can be imaged using any type of microscope, producing high-resolution multiplex images. Alexa Fluor™ 568 Tyramide Reagent (579/604 ex/em) can be detected using a standard Orange/RFP/TRIC filter cube.

Features of the SuperBoost reagents include:
• Superior sensitivity for detection of low-level or hard-to-detect targets by fluorescent imaging
• Simple protocol and detection using standard filters
• Suitable for high-resolution multiplex images—co-label with TO-PRO™-3, secondary antibodies, and other SuperBoost kits
• Requires 10-100 times less primary antibody then standard ICC/IHC/ISH experiments

Biotin-XX Tyramide Reagent (Invitrogen™)

Biotin XX Tyramide Reagent is a component of SuperBoost™ tyramide signal amplification kits made available separately here. This reagent can be used with any antibody conjugated to HRP for tyramide signal amplification for detection of low abundance targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH). For detection and additional signal amplification, conjugated streptavidin should be used to detect specifically deposited biotin XX.

SuperBoost reagents, when used as directed, can increase sensitivity up to 200 times over standard imaging methods. For standout research, SuperBoost reagents sharpen your results for clear visibility into critical areas that standard imaging methods fail to reveal. SuperBoost reagents are simple to use and easily adapted to standard ICC, IHC, or FISH experimental protocols, using any cell or tissue type. Cells labeled using a SuperBoost reagent can be imaged using any type of microscope, producing high-resolution multiplex images.

Features of the SuperBoost reagents include:
• Superior sensitivity for detection of low-level or hard-to-detect targets by fluorescent imaging
• Simple protocol and detection using standard filters
• Suitable for high-resolution multiplex images—co-label with TO-PRO™-3, secondary antibodies, and other SuperBoost kits
• Requires 10-100 times less primary antibody then standard ICC/IHC/ISH experiments

TRA-1-60 Alexa Fluor™ 555 Conjugate Kit for Live Cell Imaging (Invitrogen™)

The Molecular Probes® human pluripotent stem cell (PSC) live imaging kits are the only kits offering superior imaging for live human PSCs in one box. This TRA-1-60 Alexa Fluor® 555 Conjugate Kit includes an optimally labeled primary antibody paired with reduced-background imaging medium (FluoroBrite™ DMEM) for the specific detection of the PSC marker TRA-1-60. For fixed cell staining of PSC markers, we recommend the Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit.

With the TRA-1-60 Alexa Fluor® 555 Conjugate Kit for Live Cell Imaging, you can:
• Confirm the expression of a key pluripotent stem cell marker using an optimally dye-conjugated antibody
• Preserve cell health and maximize fluorescence signal with FluoroBrite™ DMEM imaging medium
• Stain live cell samples with confidence using reagents that are tested to be free of common contaminants

Confirm Expression of a Key PSC Marker
Powered by the high performance of bright, photostable Alexa Fluor® dyes, this kit enables reliable live-cell detection of the surface PSC marker TRA-1-60. This antibody conjugate has been demonstrated to show superior staining results when used with FluoroBrite™ DMEM imaging medium.

Preserve Cell Health and Maximize Signal
Staining cells in FluoroBrite™ DMEM imaging medium maximizes cell health and enhances signal-to-noise ratio when performing live-cell fluorescence imaging.

Stain Live-cell Samples with Confidence
Unlike most other conjugated antibodies, the reagents provided in this kit are 0.2 µm sterile-filtered and tested to be free of common contaminants.

What You Get
Easily scalable to any culture plate, dish, or slide format, the TRA-1-60 Alexa Fluor® 555 Conjugate Kit comes with reagents sufficient to stain 50 samples using a 200 uL staining volume and includes:
• 200 µL of Alexa Fluor® 555 Mouse Anti-Human TRA-1-60 (50X concentration)
• 500 mL FluoroBrite™ DMEM

Alexa Fluor™ 594 Tyramide Reagent (Invitrogen™)

Alexa Fluor™ 594 Tyramide Reagent is a component of SuperBoost™ tyramide signal amplification kits made available separately here. This reagent can be used with any antibody conjugated to HRP for tyramide signal amplification for detection of low abundance targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH).

SuperBoost reagents, when used as directed, can increase sensitivity up to 200 times over standard imaging methods. For standout research, SuperBoost reagents sharpen your results for clear visibility into critical areas that standard imaging methods fail to reveal. SuperBoost reagents are simple to use and easily adapted to standard ICC, IHC, or FISH experimental protocols, using any cell or tissue type. Cells labeled using a SuperBoost reagent can be imaged using any type of microscope, producing high-resolution multiplex images. Alexa Fluor™ 594 Tyramide Reagent (591/617 ex/em) can be detected using a standard Texas Red™ filter cube.

Features of the SuperBoost reagents include:
• Superior sensitivity for detection of low-level or hard-to-detect targets by fluorescent imaging
• Simple protocol and detection using standard filters
• Suitable for high-resolution multiplex images—co-label with TO-PRO™-3, secondary antibodies, and other SuperBoost kits
• Requires 10-100 times less primary antibody then standard ICC/IHC/ISH experiments

Alexa Fluor™ 488 Tyramide SuperBoost™ Kit, goat anti-mouse IgG (Invitrogen™)

SuperBoost™ tyramide signal amplification is the most sensitive method for detection of low abundant targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH). SuperBoost kits combine the brightness of AlexaFluor™ dyes with the superior signal amplification of a poly-HRP-mediated tyramide labeling reaction to produce a sensitivity 10-200 times greater than standard methods. SuperBoost kit sensitivity is also 2-10 times greater than regular tyramide amplification techniques like TSA™. For standout research, SuperBoost kits sharpen your results for clear visibility into critical areas that standard imaging methods fail to reveal.

SuperBoost kits are simple to use and easily adapted to standard ICC, IHC, or FISH experimental protocols, using any cell or tissue type. Cells labeled using a SuperBoost kit can be imaged using any type of microscope, producing high-resolution multiplex images. This particular kit features AlexaFluor 488 tyramide (496/524 ex/em), detected using a standard Green/FITC/GFP filter cube. This kit also features poly-HRP-conjugated goat anti-mouse IgG secondary antibody.

Features of the SuperBoost kits include:
• Superior sensitivity for detection of low-level or hard-to-detect targets by fluorescent imaging
• Simple protocol and detection using standard filters
• Suitable for high-resolution multiplex images—co-label with DAPI, secondary antibodies, and other SuperBoost kits
• Requires 10-100 times less primary antibody then standard ICC/IHC/ISH experiments

SuperBoost kits are based on the tyramide signal amplification system, which uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ. A typical ICC/IHC/ISH experiment using a SuperBoost kit requires 10-100 times less primary antibody then standard ICC/IHC/ISH experiments. SuperBoost kits offer superior specific signal intensity over background, so the protocol is easily optimized to detect specific signal in samples where high endogenous autofluorescence is observed.

Benefits of SuperBoost kits

Enhancement of signal using Alexa Fluor tyramides: SuperBoost kits utilize Alexa Fluor tyramides, which react with HRP to ultimately deposit bright and photostable Alexa Fluor dye on surrounding proteins and other similar molecules. SuperBoost kits are the only kits that combine the brightness of Alexa Fluor dyes with the enhancement of tyramide signal amplification to produce a superior signal.

Poly-HRP enhancement: Unlike TSA, SuperBoost kits employ poly-HRP-conjugated secondary antibodies. In such systems, several HRP enzymes are conjugated with short polymers, enhancing the signal by several fold over regular HRP systems. The poly-HRP is structured in such a way that the antibodies penetrate cells or tissue as efficiently as regular HRP-conjugated secondary antibodies. The molar enzyme/antibody protein ratio has an average value of '4'.

Reaction stop solution: Like any enzyme-based labeling system, it is possible to overdevelop the signal. SuperBoost kits include an HRP stop solution to halt the HRP reaction. HRP stop solution can be used to obtain maximum signal, without increase of background signal. Images produced with optimized HRP reaction times are as sharp as images produced with standard ICC/IHC/ISH methods, but with 10-200 times more sensitivity.

Reduction of background: SuperBoost kits include blockers for the elimination or reduction of endogenous peroxidase and fluorescent background signals. These blockers help ensure that only specific signals are enhanced while keeping non-specific/background signals in check.

Alexa Fluor™ 647 Goat Anti-Rabbit SFX Kit, highly cross-adsorbed (Invitrogen™)

Alexa Fluor® SFX Kits contain Image-iT® FX signal enhancer (Cat. no. I36933) plus one of sixteen different Alexa Fluor® dye-labeled secondary antibodies. These kits provide 400 µg (0.2 mL of 2 mg/mL) of either goat anti-mouse IgG or goat anti-rabbit IgG antibody (as a standard or highly cross-adsorbed preparation) conjugated to Alexa Fluor® 488, Alexa Fluor® 555, Alexa Fluor® 594, or Alexa Fluor® 647 dye, four of our most commonly used Alexa Fluor® dyes. The Alexa Fluor® 647 goat anti-rabbit IgG, highly cross-adsorbed, included with this kit is also available in a 1000 µg unit size (Cat. no. A21245).

Alexa Fluor™ 488 Tyramide SuperBoost™ Kit, goat anti-rabbit IgG (Invitrogen™)

SuperBoost™ tyramide signal amplification is the most sensitive method for detection of low abundant targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH). SuperBoost kits combine the brightness of AlexaFluor™ dyes with the superior signal amplification of a poly-HRP-mediated tyramide labeling reaction to produce a sensitivity 10-200 times greater than standard methods. SuperBoost kit sensitivity is also 2-10 times greater than regular tyramide amplification techniques like TSA™. For standout research, SuperBoost kits sharpen your results for clear visibility into critical areas that standard imaging methods fail to reveal.

SuperBoost kits are simple to use and easily adapted to standard ICC, IHC, or FISH experimental protocols, using any cell or tissue type. Cells labeled using a SuperBoost kit can be imaged using any type of microscope, producing high-resolution multiplex images. This particular kit features AlexaFluor 488 tyramide (496/524 ex/em), detected using a standard Green/FITC/GFP filter cube. This kit also features poly-HRP-conjugated goat anti-rabbit IgG secondary antibody.

Features of the SuperBoost kits include:
• Superior sensitivity for detection of low-level or hard-to-detect targets by fluorescent imaging
• Simple protocol and detection using standard filters
• Suitable for high-resolution multiplex images—co-label with DAPI, secondary antibodies, and other SuperBoost kits
• Requires 10-100 times less primary antibody then standard ICC/IHC/ISH experiments

SuperBoost kits are based on the tyramide signal amplification system, which uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ. A typical ICC/IHC/ISH experiment using a SuperBoost kit requires 10-100 times less primary antibody then standard ICC/IHC/ISH experiments. SuperBoost kits offer superior specific signal intensity over background, so the protocol is easily optimized to detect specific signal in samples where high endogenous autofluorescence is observed.

Benefits of SuperBoost kits

Enhancement of signal using Alexa Fluor tyramides: SuperBoost kits utilize Alexa Fluor tyramides, which react with HRP to ultimately deposit bright and photostable Alexa Fluor dye on surrounding proteins and other similar molecules. SuperBoost kits are the only kits that combine the brightness of Alexa Fluor dyes with the enhancement of tyramide signal amplification to produce a superior signal.

Poly-HRP enhancement: Unlike TSA, SuperBoost kits employ poly-HRP-conjugated secondary antibodies. In such systems, several HRP enzymes are conjugated with short polymers, enhancing the signal by several fold over regular HRP systems. The poly-HRP is structured in such a way that the antibodies penetrate cells or tissue as efficiently as regular HRP-conjugated secondary antibodies. The molar enzyme/antibody protein ratio has an average value of '4'.

Reaction stop solution: Like any enzyme-based labeling system, it is possible to overdevelop the signal. SuperBoost kits include an HRP stop solution to halt the HRP reaction. HRP stop solution can be used to obtain maximum signal, without increase of background signal. Images produced with optimized HRP reaction times are as sharp as images produced with standard ICC/IHC/ISH methods, but with 10-200 times more sensitivity.

Reduction of background: SuperBoost kits include blockers for the elimination or reduction of endogenous peroxidase and fluorescent background signals. These blockers help ensure that only specific signals are enhanced while keeping non-specific/background signals in check.

Alexa Fluor™ 350 Tyramide Reagent (Invitrogen™)

Alexa Fluor™ 350 Tyramide Reagent is a component of SuperBoost™ tyramide signal amplification kits made available separately here. This reagent can be used with any antibody conjugated to HRP for tyramide signal amplification for detection of low abundance targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH).

SuperBoost reagents, when used as directed, can increase sensitivity up to 200 times over standard imaging methods. For standout research, SuperBoost reagents sharpen your results for clear visibility into critical areas that standard imaging methods fail to reveal. SuperBoost reagents are simple to use and easily adapted to standard ICC, IHC, or FISH experimental protocols, using any cell or tissue type. Cells labeled using a SuperBoost reagent can be imaged using any type of microscope, producing high-resolution multiplex images. Alexa Fluor™ 350 Tyramide Reagent (347/442 ex/em) can be detected using a standard DAPI filter cube.

Features of the SuperBoost reagents include:
• Superior sensitivity for detection of low-level or hard-to-detect targets by fluorescent imaging
• Simple protocol and detection using standard filters
• Suitable for high-resolution multiplex images—co-label with TO-PRO™-3, secondary antibodies, and other SuperBoost kits
• Requires 10-100 times less primary antibody then standard ICC/IHC/ISH experiments

SuperBoost™ Goat anti-Rabbit Poly HRP (Invitrogen™)

SuperBoost™ Goat anti-Rabbit Poly HRP is a component in SuperBoost™ tyramide signal amplification kits made available separately here. SuperBoost™ Goat anti-Rabbit Poly HRP can be used with any HRP sustrate such as tyramide, DAB for signal amplification and detection. The molar enzyme/antibody protein ratio has an average value of '4'. This reagent is provided at a ready-to-use 1X concentration, enough for 150 slides.

When used as directed, this reagent with other SuperBoost reagents can be used for detection of low abundant targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH) with up to 200 times more sensitivity than standard methods. For standout research, SuperBoost reagents sharpen your results for clear visibility into critical areas that standard imaging methods fail to reveal. SuperBoost reagents are simple to use and easily adapted to standard ICC, IHC, or FISH experimental protocols, using any cell or tissue type. Cells labeled using a SuperBoost reagent can be imaged using any type of microscope, producing high-resolution multiplex images.

Features of the SuperBoost reagents include:
• Superior sensitivity for detection of low-level or hard-to-detect targets by fluorescent imaging
• Simple protocol and detection using standard filters
• Suitable for high-resolution multiplex images—co-label with TO-PRO™-3, secondary antibodies, and other SuperBoost kits
• Requires 10-100 times less primary antibody then standard ICC/IHC/ISH experiments

Diaminobenzidine (DAB) Histochemistry Kit #3, with Streptavidin—HRP (Invitrogen™)

The DAB Histochemistry Kit for biotinylated probes contains sufficient materials to stain approximately 200 slides using horseradish peroxidase (HRP) and the HRP substrate DAB. The brown-colored DAB reaction product can be visualized by bright-fieldd light microscopy or, following osmication, by electron microscopy.

Alexa Fluor™ 647 Tyramide SuperBoost™ Kit, goat anti-mouse IgG (Invitrogen™)

SuperBoost™ tyramide signal amplification is the most sensitive method for detection of low abundant targets in multiplexable fluorescent immunocytochemistry (ICC), immunohistochemistry (IHC ), and in situ hybridization (ISH). SuperBoost kits combine the brightness of AlexaFluor™ dyes with the superior signal amplification of a poly-HRP-mediated tyramide labeling reaction to produce a sensitivity 10-200 times greater than standard methods. SuperBoost kit sensitivity is also 2-10 times greater than regular tyramide amplification techniques like TSA™. For standout research, SuperBoost kits sharpen your results for clear visibility into critical areas that standard imaging methods fail to reveal.

SuperBoost kits are simple to use and easily adapted to standard ICC, IHC, or FISH experimental protocols, using any cell or tissue type. Cells labeled using a SuperBoost kit can be imaged using any type of microscope, producing high-resolution multiplex images. This particular kit features AlexaFluor 647 tyramide (650/688 ex/em), detected using a standard Deep Red/Cy5 filter cube. This kit also features poly-HRP-conjugated goat anti-mouse IgG secondary antibody.

Features of the SuperBoost kits include:
• Superior sensitivity for detection of low-level or hard-to-detect targets by fluorescent imaging
• Simple protocol and detection using standard filters
• Suitable for high-resolution multiplex images—co-label with DAPI, secondary antibodies, and other SuperBoost kits
• Requires 10-100 times less primary antibody then standard ICC/IHC/ISH experiments

SuperBoost kits are based on the tyramide signal amplification system, which uses the catalytic activity of horseradish peroxidase (HRP) to generate high density labeling of a target protein or nucleic acid sequence in situ. A typical ICC/IHC/ISH experiment using a SuperBoost kit requires 10-100 times less primary antibody then standard ICC/IHC/ISH experiments. SuperBoost kits offer superior specific signal intensity over background, so the protocol is easily optimized to detect specific signal in samples where high endogenous autofluorescence is observed.

Benefits of SuperBoost kits

Enhancement of signal using Alexa Fluor tyramides: SuperBoost kits utilize Alexa Fluor tyramides, which react with HRP to ultimately deposit bright and photostable Alexa Fluor dye on surrounding proteins and other similar molecules. SuperBoost kits are the only kits that combine the brightness of Alexa Fluor dyes with the enhancement of tyramide signal amplification to produce a superior signal.

Poly-HRP enhancement: Unlike TSA, SuperBoost kits employ poly-HRP-conjugated secondary antibodies. In such systems, several HRP enzymes are conjugated with short polymers, enhancing the signal by several fold over regular HRP systems. The poly-HRP is structured in such a way that the antibodies penetrate cells or tissue as efficiently as regular HRP-conjugated secondary antibodies. The molar enzyme/antibody protein ratio has an average value of '4'.

Reaction stop solution: Like any enzyme-based labeling system, it is possible to overdevelop the signal. SuperBoost kits include an HRP stop solution to halt the HRP reaction. HRP stop solution can be used to obtain maximum signal, without increase of background signal. Images produced with optimized HRP reaction times are as sharp as images produced with standard ICC/IHC/ISH methods, but with 10-200 times more sensitivity.

Reduction of background: SuperBoost kits include blockers for the elimination or reduction of endogenous peroxidase and fluorescent background signals. These blockers help ensure that only specific signals are enhanced while keeping non-specific/background signals in check.

Foxp3 Transcription Factor Staining Buffer Kit with Foxp3 Mouse Anti-Mouse mAb (clone 3G3), FITC conjugate (Invitrogen™)

This product consists of a Foxp3 Transcription Factor Staining Buffer Kit (A24261) and a Foxp3 Mouse anti-Mouse mAb (clone 3G3), FITC conjugate (A18662).

About the Antibody
The Foxp3 Mouse Anti-mouse mAb (clone 3G3), FITC conjugate, is a monoclonal antibody that reacts with mouse Foxp3 protein. Commonly used, FITC conjugates provide relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility. FITC conjugates have been superseded by the Alexa Fluor® 488 dye conjugates, which exhibit a fluorescence emission profile almost identical to FITC and provide pH-insensitive fluorescence between pH 4 and 10.

About the Buffers
The buffers in the included Foxp3 Transcription Factor Buffer Kit have been specially formulated to work with the FITC conjugate of Foxp3 Mouse Anti-Mouse mAb (clone 3G3) for optimal observation of intranuclear staining when used in flow cytometry applications. The Foxp3 Transcription Factor Buffer Kit is compatible with intracellular cytokine staining within the same panel.

The kit contains 3 buffers:
• Foxp3 Fixation Transcription Factor and Permeabilization Buffer A (A24217, a 4X concentrate)
• Foxp3 Transcription Factor Fixation and Permeabilization Buffer B (A24218, a diluent)
• Foxp3 Transcription Factor Wash Buffer (A24261, 10X)

How the Buffers Work
The Foxp3 Transcription Factor Fixation and Permeabilization Buffer A is supplied as a 4X stock solution and must be diluted with Foxp3 Transcription Factor Fixation and Permeabilization Buffer B prior to use.

The Foxp3 Transcription Factor Wash Buffer is supplied as a 10X stock solution. The 10X stock solution should be diluted to a 1X working concentration in PBS containing 1% BSA and used for wash and intranuclear Foxp3 staining steps following fixation and permeabilization with 1X Foxp3 Fixation and Permabilization buffers.

About Foxp3
The 3G3 antibody reacts with mouse Foxp3 protein, a 50-55 kDa transcription factor that is a master regulator of regulatory T cell (Treg) development and function. Foxp3 is expressed constitutively by Tregs, which are further identified as being CD4+ CD25+, whereas resting conventional CD4+ T cells do not express Foxp3. However, TCR stimulation of naive T cells in the presence of TGF-beta has been shown to induce expression of Foxp3, driving their differentiation into Foxp3+ Tregs, which are called “induced" or “adaptive" Tregs. These cells are phenotypically similar to "natural" Tregs (CD4+ CD25+ Foxp3+), which originate in the thymus and comprise the majority of Tregs in the periphery. Tregs are critical for maintaining peripheral immune homeostasis. Defects in Foxp3 expression or Treg function have been implicated in the development of autoimmunity. Tregs have also been shown to restrict immune responses to infection, cancer, and vaccination. The 3G3 antibody may be used for intracellular detection of Foxp3 in cells from mouse and Rhesus macaque.

Related Links
Use the SpectraViewer to help you plan your experiments. Additional general information about Life Technologies® flow cytometry products and support is also available.

Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit (Invitrogen™)

The Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit enables optimal image-based analysis of four key markers of human pluripotent stem cells (PSCs): OCT4, SOX2, SSEA4, and TRA-1-60. This high performance immunocytochemistry (ICC) kit includes a complete set of primary and secondary antibodies, a nuclear DNA stain, and premade buffers for an optimized staining experiment. To characterize pluripotent stem cells using only two markers, we recommend us of the Pluripotent Stem Cell Immunocytochemistry Kit (OCT4, SSEA4) or the Pluripotent Stem Cell Immunocytochemistry Kit (SOX2, TRA-1-60).

With the Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit, you can:
• Confirm expression of key pluripotent stem cell markers with highly specific antibodies
• Get more information per sample by measuring more than one marker at a time
• Generate beautiful multiplexed images with minimal effort
• Save time and avoid cell loss by minimizing unnecessary wash steps in your staining protocol
• Process samples with confidence using a complete and optimized set of ICC reagents

Confirm Expression of Key PSC Markers
The antibodies included in the kit have been carefully selected and validated for high performance in the ICC analysis of PSCs, whether cultured under feeder-dependent or feeder-free media systems. OCT4 and SOX2 are critical nuclear markers, while SSEA4 and TRA-1-60 are important cell surface markers.

Get More Information Per Sample
The antibody combinations in the kit have been designed to allow for the specific and simultaneous assessment of two markers at a time (e.g., OCT4 & SSEA4 and SOX2 & TRA-1-60) along with nuclear DNA staining to help save time and precious sample.

Generate Beautiful Multiplexed Images
Powered by the superior performance of bright, photostable Alexa Fluor® dyes, this kit enables the acquisition of beautiful multiplexed images of PSC marker expression using commonly available blue, green, and orange/red filter sets.

Minimize Unnecessary Wash Steps
The streamlined method recommended for this kit eliminates unnecessary wash steps found in standard ICC protocols to help save time and decrease the likelihood of cell loss during the staining protocol, especially when using glass coverslips or chamber slides. The only washes required are after incubation with primary and secondary antibodies.

Process Samples with Confidence
An optimized set of premade fixation, permeabilization, blocking, and wash buffers allows you to focus on answering scientific questions rather than spending time preparing reagents and determining compatibility.

What You Get
Easily scaled to any culture plate, dish, or slide format, the Pluripotent Stem Cell 4-Marker Immunocytochemistry Kit comes with reagents sufficient to stain 40 samples using a 50 uL staining volume. (See kit content details below.)

ELF™ 97 mRNA In Situ Hybridization Kit #1 (Invitrogen™)

The ELF® 97 mRNA In Situ Hybridization Kit #1 provides a rapid and sensitive assay for detecting mRNA in situ hybridization signals in cells and tissue sections. The kit is designed to detect alkaline phosphatase conjugates that have been applied to detect DNA or RNA probes utilizing the ELF® 97 phosphatase substrate that upon hydrolysis produces a bright and photostable yellow-green fluorescent precipitate at the site of enzymatic activity. This fluorescent precipitate has several unique spectral characteristics, including an extremely large Stokes shift, 180 nm that makes it easily distinguishable from endogenous fluorescence.