Meat Species Detection and Quantification

RapidFinder™ Quant Equine Set

The RapidFinder™ Quant Equine Set, when used with the RapidFinder™ Equine ID Kit, enables the fast and accurate quantification of horse meat in food and feed samples. Using the included standards, the kit enables quantification of as little as 0.05% horse meat DNA relative to other meat DNA in only a few hours.

• Simple—real-time PCR based assay, no electrophoresis needed
• Flexible—compatible with any food or feed sample, both raw and processed
• Reliable—includes equine DNA quantification standard
• Fast—from sample to result on the same day

How It Works
The kit enables the quantitative real-time PCR-based generation of a standard curve using the included Equus caballus DNA standard. Then assays for both equine DNA and all meat DNA, detected in the FAM™ channel, are run side by side on both food samples and the standards. This approach enables rapid and accurate quantification and reporting of the percentage of horse meat as compared to other meat species in a sample. Included in each reaction mix is an internal positive control detected in the VIC® channel, which is used to verify the absence of PCR inhibitors.

The RapidFinder™ Quant Equine Set is designed to be used with the RapidFinder™ Equine ID Kit, and is compatible with Applied Biosystems® StepOne™, StepOnePlus™, 7500, 7500 Fast, and 7300 Real-time PCR Systems.

Sample Preparation
DNA sample preparation for the RapidFinder™ Equine ID Kit and RapidFinder™ Quant Equine Set workflow is accomplished with the easy-to-use GMO Extraction Kit. Using 10 g of sample rather than smaller sample sizes accommodated by most other DNA isolation procedures minimizes sampling errors. The GMO Extraction Kit is compatible with raw and processed meat, as well as composite food samples.

RapidFinder™ Ruminant ID Kit

The RapidFinder™ Ruminant ID Kit enables the simple and reliable detection of ruminant meat in food and feed samples. The method works by detection of Bos taurus, Ovis aries, Capra hircus, Cervus elaphus, and Capreolus capreolus DNA, which corresponds to DNA from beef, sheep, goat, and deer, by real-time PCR. Unlike ELISA based methods, when used with the GMO Extraction Kit (Cat. No. 4466336) for sample preparation, the RapidFinder™ Ruminant ID Kit detects both raw and processed ruminant meat and provides sensitivity down to 0.01% ruminant DNA. The included positive control in the kit, which comprises 0.1% ruminant DNA, can be used as a reference to set the threshold of detection of the assay.

• Simple-real-time PCR based assay, no electrophoresis needed
• Flexible-ruminant DNA detected in any food or feed sample, both raw and processed
• Reliable-Internal Positive Control (IPC) provides process control check for PCR inhibitors
• Fast-from sample to result in the same day

How the RapidFinder™ Ruminant ID Kit Works
Ruminant DNA detection is accomplished by real-time PCR using the supplied primer/probe mixes targeting a multi-copy nuclear target specific to Bos taurus, Ovis aries, Capra hircus, Cervus elaphus, and Capreolus capreolus. The primer/probes are supplied in a single tube, so if any of the five included ruminant species is detected, a positive result will be obtained. The target sequences, detected in the FAM dye channel, are not only specific for the five ruminants, but are also stable. Also included in each reaction mix is an internal positive control detected in the VIC dye channel, which is used to verify the absence of PCR inhibitors.

By performing a separate reaction with the included positive control, users are able to verify the system and assay are working correctly. More importantly, however, it enables easy data interpretation. The positive control is provided as 0.1% Bos taurus DNA in a background of fish DNA, and by comparing sample results to the control results, a threshold of detection is provided.

The assay is compatible with Applied Biosystems® StepOne™, StepOnePlus™, 7500, 7500 Fast, and 7300 real-time PCR systems.

Sample Preparation
DNA sample preparation for the RapidFinder™ Ruminant ID Kit workflow is accomplished with the easy-to-use GMO Extraction Kit (Cat. No. 4466336). Using 10 g of sample rather than the smaller sample sizes accommodated by most other DNA isolation procedures minimizes sampling errors. The GMO Extraction Kit is compatible with raw and processed meat, as well as composite food samples.

Part of a Suite of Solutions For Meat Species Detection
The RapidFinder™ Ruminant ID Kit is just one of our many available kits for the sensitive detection of various meat species in food and feed samples. Other RapidFinder™ meat species identification kits available include those for the detection of pork, beef, horse, sheep, chicken, turkey, and poultry.

The RapidFinder™ Meat Species Identification Kits are made available through a partnership with IMEGEN (Instituto de Medicina Genomica).

RapidFinder™ Beef ID Kit

The RapidFinder™ Beef ID Kit enables the simple and reliable detection of beef in food and feed samples. The method works by detection of bovine, or Bos taurus, DNA by real-time PCR. Unlike ELISA-based methods, when used with the GMO Extraction Kit (Cat. No. 4466336) for sample preparation, the RapidFinder™ Beef ID Kit detects both raw and processed beef and provides sensitivity down to 0.01% bovine DNA. The included positive control, which comprises 0.1% bovine DNA, can be used as a reference to set the threshold of detection of the assay.

• Simple-real-time PCR based assay, no electrophoresis needed
• Flexible-bovine DNA detected in any food or feed sample, both raw and processed
• Reliable-Internal Positive Control (IPC) provides process-control check for PCR inhibitors
• Fast-from sample to result in the same day

How the RapidFinder™ Beef ID Kit Works
Bovine DNA detection is accomplished by real-time PCR using the supplied primer/probe set specific for mitochondrial DNA sequence specific to Bos taurus. The target sequence, detected in the FAM™ dye channel, is not only specific for beef, but also stable. Also included in each reaction mix is an internal positive control detected in the VIC® dye channel, which is used to verify the absence of PCR inhibitors.

By performing a separate reaction with the included positive control, users are able to verify the system and assay are working correctly. More importantly, it enables easy data interpretation. The positive control is provided as 0.1% bovine DNA in a background of fish DNA, and by comparing sample results to the control results, a threshold of detection is provided.

The assay is compatible with Applied Biosystems® StepOne™, StepOnePlus™, 7500, 7500 Fast, and 7300 real-time PCR systems.

Sample Preparation
DNA sample preparation for the RapidFinder™ Beef ID Kit workflow is accomplished with the easy-to-use GMO Extraction Kit (Cat. No. 4466336). Using 10 g of sample rather than the smaller sample sizes accommodated by most other DNA isolation procedures minimizes sampling errors. The GMO Extraction Kit is compatible with raw and processed meat, as well as composite food samples.

Quantification
If a positive result is obtained with the RapidFinder™ Beef ID Kit, most labs will go on to quantify the amount of bovine DNA in the sample. This can be accomplished using the RapidFinder™ Beef ID Kit in conjunction with the RapidFinder™ Quant Multi-Meat Set (Cat. No. A24399).

Part of a Suite of Solutions For Meat Species Detection
The RapidFinder™ Beef ID Kit is just one of our many kits available for the sensitive detection of various meat species in food and feed samples. Other RapidFinder™ meat species identification kits available include those for the detection of pork, horse, sheep, ruminant, chicken, turkey, and poultry.

The RapidFinder™ Meat Species Identification kits are made available through a partnership with IMEGEN (Instituto de Medicina Genomica).

RapidFinder™ Chicken ID Kit

The RapidFinder™ Chicken ID Kit enables the simple and reliable detection of chicken in food and feed samples. The method works by detection of chicken, or Gallus gallus, DNA by real-time PCR. Unlike ELISA based methods, when used with the GMO Extraction Kit (Cat. No. 4466336) for sample preparation, the RapidFinder™ Chicken ID Kit detects both raw and processed chicken and provides sensitivity down to 0.01% chicken DNA. The included positive control, which comprises 0.1% chicken DNA, can be used as a reference to set the threshold of detection of the assay.

• Simple-real-time PCR based assay, no electrophoresis needed
• Flexible-chicken DNA detected in any food or feed sample, both raw and processed
• Reliable-Internal Positive Control (IPC) provides process control check for PCR inhibitors
• Fast-from sample to result in the same day

How the RapidFinder™ Chicken ID Kit Works
Chicken DNA detection is accomplished by real-time PCR using the supplied primer/probe set specific for mitochondrial DNA sequence specific to Gallus gallus. The target sequence, detected in the FAM™ dye channel, is not only specific for chicken, but also stable. Also included in each reaction mix is an internal positive control detected in the VIC® dye channel, which is used to verify the absence of PCR inhibitors.

By performing a separate reaction with the included positive control, users are able to verify the system and assay are working correctly. More importantly, it enables easy data interpretation. The positive control is provided as 0.1% chicken DNA in a background of fish DNA, and by comparing sample results to the control results, a threshold of detection is provided.

The assay is compatible with Applied Biosystems® StepOne™, StepOnePlus™, 7500, 7500 Fast, and 7300 real-time PCR systems.

Sample Preparation
DNA sample preparation for the RapidFinder™ Chicken ID Kit workflow is accomplished with the easy-to-use GMO Extraction Kit (Cat. No. 4466336). Using 10 g of sample rather than the smaller sample sizes accommodated by most other DNA isolation procedures minimizes sampling errors. The GMO Extraction Kit is compatible with raw and processed meat, as well as composite food samples.

Quantification
If a positive result is obtained with the RapidFinder™ Chicken ID Kit, most labs will go on to quantify the amount of chicken DNA in the sample. This can be accomplished using the RapidFinder™ Chicken ID Kit in conjunction with the RapidFinder™ Quant Multi-Meat Set (Cat. No. A24399).

Part of a Suite of Solutions For Meat Species Detection
The RapidFinder™ Chicken ID Kit is just one of our many kits available for the sensitive detection of various meat species in food and feed samples. Other RapidFinder™ meat species identification kits available include those for the detection of pork, beef, horse, sheep, ruminant, turkey, and poultry.

The RapidFinder™ Meat Species Identification Kits are made available through a partnership with IMEGEN (Instituto de Medicina Genomica).

RapidFinder™ Poultry ID Kit

The RapidFinder™ Poultry ID Kit enables the simple and reliable detection of poultry meat in food and feed samples. The method works by detection of avian DNA, including DNA from chicken, turkey, duck, ostrich, goose, and other birds, by real-time PCR. Unlike ELISA based methods, when used with the GMO Extraction Kit (Cat. No. 4466336) for sample preparation, the RapidFinder™ Poultry ID Kit detects both raw and processed poultry meat and provides sensitivity down to 0.01% poultry DNA. The included positive control in the kit, which comprises 0.1% poultry DNA, can be used as a reference to set the threshold of detection of the assay.

• Simple-real-time PCR based assay, no electrophoresis needed
• Flexible-poultry DNA detected in any food or feed sample, both raw and processed
• Reliable-Internal Positive Control (IPC) provides process control check for PCR inhibitors
• Fast-from sample to result in the same day

How the RapidFinder™ Poultry ID Kit Works
Poultry DNA detection is accomplished by real-time PCR using the supplied primer/probe set targeting mitochondrial DNA sequence specific to several avian species, including but not limited to Gallus gallus (chicken), Meleagris gallopavo (turkey), Anas platyrhynchos (duck), Struthio camelus (ostrich), and Anser (goose). The primer/probes are supplied in a single tube, so if any of the poultry species is detected, a positive result will be obtained. Also included in each reaction mix is an internal positive control detected in the VIC® dye channel, which is used to verify the absence of PCR inhibitors.

By performing a separate reaction with the included positive control, users are able to verify the system and assay are working correctly. More importantly, it enables easy data interpretation. The positive control is provided as 0.1% Gallus gallus DNA in a background of fish DNA, and by comparing sample results to the control results, a threshold of detection is provided.

The assay is compatible with Applied Biosystems® StepOne™, StepOnePlus™, 7500, 7500 Fast, and 7300 real-time PCR systems.

Sample Preparation
DNA sample preparation for the RapidFinder™ Poultry ID Kit workflow is accomplished with the easy-to-use GMO Extraction Kit (Cat. No. 4466336). Using 10 g of sample rather than the smaller sample sizes accommodated by most other DNA isolation procedures minimizes sampling errors. The GMO Extraction Kit is compatible with raw and processed meat, as well as composite food samples.

Quantification
If a positive result is obtained with the RapidFinder™ Poultry ID Kit, most labs will go on to quantify the amount of poultry DNA in the sample. This can be accomplished using the RapidFinder™ Poultry ID Kit in conjunction with the RapidFinder™ Quant Multi-Meat Set (Cat. No. A24399).

Part of a Suite of Solutions For Meat Species Detection
The RapidFinder™ Poultry ID Kit is just one of our many available kits for the sensitive detection of various meat species in food and feed samples. Other RapidFinder™ meat species identification kits available include those for the detection of pork, beef, horse, sheep, ruminant, chicken, and turkey.

The RapidFinder™ Meat Species Identification Kits are made available through a partnership with IMEGEN (Instituto de Medicina Genomica).

RapidFinder™ Sheep ID Kit

The RapidFinder™ Sheep ID Kit enables the simple and reliable detection of sheep and lamb in food and feed samples. The method works by detection of ovine, or Ovis aries, DNA by real-time PCR. Unlike ELISA based methods, when used with the GMO Extraction Kit (Cat. No. 4466336) for sample preparation, the RapidFinder™ Sheep ID Kit detects both raw and processed sheep and lamb and provides sensitivity down to 0.01% ovine DNA. The included positive control in the kit, which comprises 0.1% ovine DNA, can be used as a reference to set the threshold of detection of the assay.

• Simple-real-time PCR based assay, no electrophoresis needed
• Flexible-ovine DNA detected in any food or feed sample, both raw and processed
• Reliable-Internal Positive Control (IPC) provides process control check for PCR inhibitors
• Fast-from sample to result in the same day

How the RapidFinder™ Sheep ID Kit Works
Ovine DNA detection is accomplished by real-time PCR using the supplied primer/probe set specific for mitochondrial DNA sequence specific to Ovis aries. The target sequence, detected in the FAM™ dye channel, is not only specific for sheep, but also stable. Also included in each reaction mix is an internal positive control detected in the VIC® dye channel, which is used to verify the absence of PCR inhibitors.

By performing a separate reaction with the included positive control, users are able to verify the system and assay are working correctly. More importantly, it enables easy data interpretation. The positive control is provided as 0.1% ovine DNA in a background of fish DNA, and by comparing sample results to the control results, a threshold of detection is provided.

The assay is compatible with Applied Biosystems® StepOne™, StepOnePlus™, 7500, 7500 Fast, and 7300 real-time PCR systems.

Sample Preparation
DNA sample preparation for the RapidFinder™ Sheep ID Kit workflow is accomplished with the easy-to-use GMO Extraction Kit (Cat. No. 4466336). Using 10 g of sample rather than the smaller sample sizes accommodated by most other DNA isolation procedures minimizes sampling errors. The GMO Extraction Kit is compatible with raw and processed meat, as well as composite food samples.

Part of a Suite of Solutions For Meat Species Detection
The RapidFinder™ Sheep ID Kit is just one of our many available kits for the sensitive detection of various meat species in food and feed samples. Other RapidFinder™ meat species identification kits available include those for the detection of pork, beef, horse, ruminant, chicken, turkey, and poultry.

The RapidFinder™ Meat Species Identification Kits are made available through a partnership with IMEGEN (Instituto de Medicina Genomica).

RapidFinder™ Pork ID Kit

The RapidFinder™ Pork ID Kit enables the simple and reliable detection of pork in food and feed samples. The method works by detection of porcine, or Sus scrofa, DNA by real-time PCR. Unlike ELISA based methods, when used with the GMO Extraction Kit (Cat. No. 4466336) for sample preparation, the RapidFinder™ Pork ID Kit detects both raw and processed pork and provides sensitivity down to 0.01% porcine DNA. The included positive control, which comprises 0.1% porcine DNA, can be used as a reference to set the threshold of detection of the assay.

• Simple-real-time PCR based assay, no electrophoresis needed
• Flexible-porcine DNA detected in any food or feed sample, both raw and processed
• Reliable-Internal Positive Control (IPC) provides process control check for PCR inhibitors
• Fast-from sample to result in the same day

How the RapidFinder™ Pork ID Kit Works
Porcine DNA detection is accomplished by real-time PCR using the supplied primer/probe set specific for mitochondrial DNA sequence specific to Sus scrofa (including Sus scrofa domesticus, commonly referred to as Sus domesticus). The target sequence, detected in the FAM™ dye channel, is not only specific for pork, but also stable. Also included in each reaction mix is an internal positive control detected in the VIC® dye channel, which is used to verify the absence of PCR inhibitors.

By performing a separate reaction with the included positive control, users are able to verify the system and assay are working correctly. More importantly, it enables easy data interpretation. The positive control is provided as 0.1% porcine DNA in a background of fish DNA, and by comparing sample results to the control results, a threshold of detection is provided.

The assay is compatible with Applied Biosystems® StepOne™, StepOnePlus™, 7500, 7500 Fast, and 7300 real-time PCR systems.

Sample Preparation
DNA sample preparation for the RapidFinder™ Pork ID Kit workflow is accomplished with the easy-to-use GMO Extraction Kit (Cat. No. 4466336). Using 10 g of sample rather than the smaller sample sizes accommodated by most other DNA isolation procedures minimizes sampling errors. The GMO Extraction Kit is compatible with raw and processed meat, as well as composite food samples.

Quantification
If a positive result is obtained with the RapidFinder™ Pork ID Kit, most labs will go on to quantify the amount of porcine DNA in the sample. This can be accomplished using the RapidFinder™ Pork ID Kit in conjunction with the RapidFinder™ Quant Multi-Meat Set (Cat. No. A24399).

Part of a Suite of Solutions For Meat Species Detection
The RapidFinder™ Pork ID Kit is just one of our many available kits for the sensitive detection of various meat species in food and feed samples. Other RapidFinder™ meat species identification kits available include those for the detection of beef, horse, sheep, ruminant, chicken, turkey, and poultry.

The RapidFinder™ Meat Species Identification kits are made available through a partnership with IMEGEN (Instituto de Medicina Genomica).

RapidFinder™ Turkey ID Kit

The RapidFinder™ Turkey ID Kit enables the simple and reliable detection of turkey in food and feed samples. The method works by detection of turkey, or Meleagris gallopavo, DNA by real-time PCR. Unlike ELISA based methods, when used with the GMO Extraction Kit (Cat. No. 4466336) for sample preparation, the RapidFinder™ Turkey ID Kit detects both raw and processed turkey and provides sensitivity down to 0.01% turkey DNA. The included positive control in the kit, which comprises 0.1% turkey DNA, can be used as a reference to set the threshold of detection of the assay.

• Simple-real-time PCR based assay, no electrophoresis needed
• Flexible-turkey DNA detected in any food or feed sample, both raw and processed
• Reliable-Internal Positive Control (IPC) provides process control check for PCR inhibitors
• Fast-from sample to result in the same day

How the RapidFinder™ Turkey ID Kit Works
Turkey DNA detection is accomplished by real-time PCR using the supplied primer/probe set specific for mitochondrial DNA sequence specific to Meleagris gallopavo. The target sequence, detected in the FAM™ dye channel, is not only specific for turkey, but also stable. Also included in each reaction mix is an internal positive control detected in the VIC® dye channel, which is used to verify the absence of PCR inhibitors.

By performing a separate reaction with the included positive control, users are able to verify the system and assay are working correctly. More importantly, it enables easy data interpretation. The positive control is provided as 0.1% turkey DNA in a background of fish DNA, and by comparing sample results to the control results, a threshold of detection is provided.

The assay is compatible with Applied Biosystems® StepOne™, StepOnePlus™, 7500, 7500 Fast, and 7300 real-time PCR systems.

Sample Preparation
DNA sample preparation for the RapidFinder™ Turkey ID Kit workflow is accomplished with the easy-to-use GMO Extraction Kit (Cat. No. 4466336). Using 10 g of sample rather than smaller sample sizes accommodated by most other DNA isolation procedures minimizes sampling errors. The GMO Extraction Kit is compatible with raw and processed meat, as well as composite food samples.

Quantification
If a positive result is obtained with the RapidFinder™ Turkey ID Kit, most labs will go on to quantify the amount of turkey DNA in the sample. This can be accomplished using the RapidFinder™ Turkey ID Kit in conjunction with the RapidFinder™ Quant Multi-Meat Set (Cat. No. A24399).

Part of a Suite of Solutions For Meat Species Detection
The RapidFinder™ Turkey ID Kit is just one of our many available kits for the sensitive detection of various meat species in food and feed samples. Other RapidFinder™ meat species identification kits available include those for the detection of pork, beef, horse, sheep, ruminant, chicken, and poultry.

The RapidFinder™ Meat Species Identification Kits are made available through a partnership with IMEGEN (Instituto de Medicina Genomica).

RapidFinder™ Quant Multi-Meat Set

The RapidFinder™ Quant Multi-Meat Set, when used with one or more of the RapidFinder™ Meat ID kits listed below, enables the determination of the percentage of a target meat species with respect to total meat in food and feed samples. Using the included standards, the kit enables quantification of as little as 0.05% target species DNA as compared to other meat DNA in only a few hours.

The RapidFinder Quant Multi-Meat Set is designed to be used in combination with one or more of the following meat species-specific ID kits (available separately):

NameCatalog Number
RapidFinder™ Beef ID KitA24391
RapidFinder™ Pork ID KitA24392
RapidFinder™ Equine ID KitA15570
RapidFinder™ Poultry ID KitA24397
RapidFinder™ Chicken ID KitA24393
RapidFinder™ Turkey ID KitA24394


• Simple-real-time PCR based assay, no electrophoresis needed
• Flexible-compatible with any food or feed sample, both raw and processed
• Reliable-Includes multi-meat DNA quantification standard
• Fast-from sample to result in the same day

How It Works
The method relies on quantitative real-time PCR. First, a standard curve is created by serially diluting the included multi-meat DNA standard. Then assays for the target species DNA (from one of the kits listed above) and for all meat DNA (from the RapidFinder™ Quant Multi-Meat Set) are run side-by-side on both the food sample DNA and the DNA standards. This approach enables rapid and accurate quantification and reporting of the percentage of target meat species as compared to total meat in a sample. Included in each reaction mix is an internal positive control detected in the VIC® dye channel, which is used to verify the absence of PCR inhibitors.

The RapidFinder™ Quant Multi-Meat Set is compatible with Applied Biosystems® StepOne™, StepOnePlus™, 7500, 7500 Fast, and 7300 real-time PCR systems.

Sample Preparation
DNA sample preparation for the RapidFinder™ meat species identification and quantification workflow is accomplished with the easy-to-use GMO Extraction Kit (Cat. No. 4466336). Using 10 g of sample rather than the smaller sample sizes accommodated by most other DNA isolation procedures minimizes sampling errors. The GMO Extraction Kit is compatible with raw and processed meat, as well as composite food samples.