SMCC (succinimidyl 4-(N-maleimidomethyl)cyclohexane-1-carboxylate) - FAQs

What is the difference between SMCC and Sulfo-SMCC?

SMCC is an amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length cyclohexane-stabilized spacer arm (8.3 angstroms). Sulfo-SMCC is a similar compound but it has a sulfonate group on the NHS ring which makes it water-soluble whereas SMCC is water-insoluble and needs to be dissolved first in an organic solvent like DMF or DMSO. Subsequent dilution into aqueous reaction buffer is generally possible, and most protein reactants will remain soluble if the final concentration of organic solvent is less than 10%.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.