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Silencer™ Select Pre-Designed siRNA, 10 μmol - FAQs

View additional product information for Silencer™ Select Pre-Designed siRNA - FAQs (4404012, 4390816, 4390815, 4427037, 4444198, 4392422, 4390818, 4390817, 4390771, 4392421, 4392420)

12 product FAQs found

What controls should I use for my in vivo siRNA studies?

We offer the following in vivo siRNA controls:

Invitrogen In Vivo Negative Control #1 siRNA (Cat. No. 4457287, 5nmol; 4459405, 50 nmol; 4457289, 250 nmol)
Invitrogen In Vivo GAPDH Positive control siRNA (Cat. No. 4457288, 5 nmol; 4459407, 50 nmol, 4457291, 250 nmol)
Invitrogen In Vivo Factor VII Positive control siRNA (Cat. No. 4459408, 50 nmol; 4457292, 250 nmol)
Silencer Select Negative Control No. 1 siRNA, in vivo ready (Cat. No. 4404020, 250 nmol)
Silencer GAPDH Positive Control siRNA, in vivo ready (Cat. No. 4404025, 250 nmol)

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

How stable is Stealth siRNA, can I use it for in vivo research?

Stealth siRNA is markedly more stable in serum than unmodified siRNA. This increased stability is particularly beneficial in vivo where the RNAi may be exposed to more proteases. In collaboration with Intradigm, Stealth RNAi has been demonstrated to have activity in vivo when injected intra-tumorally.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

How can I order in vivo siRNA duplexes?

Pre-designed Stealth RNAi and Ambion In Vivo siRNA can be ordered directly from our pre-designed siRNA search interface (https://www.thermofisher.com/us/en/home/life-science/rnai/introduction-to-in-vivo-rnai.html?open=faq#search) to target human, mouse, or rat genes.

Custom siRNA designs for in vivo experiments can be ordered from two interfaces, depending on the product format being requested: Custom Ambion In Vivo siRNA is ordered from the GeneAssist Custom siRNA Builder (https://www.thermofisher.com/order/custom-genomic-products/tools/sirna/), while Stealth RNAi is ordered using the BLOCK-iT RNAi Express for in vivo synthetics (https://rnaidesigner.thermofisher.com/rnaiexpress/inVivoService.jsp).

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

Do I need endotoxin testing for my in vivo RNAi duplexes?

It is recommended to reduce the chance of eliciting an immune response.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

I want to order larger scales than the standard offering, whom do I contact?

Please contact rnairesearcher@invitrogen.com regarding large scale in vivo siRNA or fill out the quote request form (www.thermofisher.com/us/en/home/global/forms/custom-sirna-mirna-quote-request.html).

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

How should I deliver my in vivo RNAi molecules?

Several different approaches have been used for siRNA delivery, including various local delivery techniques and systemic delivery. We offer a lipid reagent, Invivofectamine 3.0 Transfection Reagent for in vivo siRNA systemic delivery. Learn more about this reagent here (https://www.thermofisher.com/us/en/home/life-science/rnai/introduction-to-in-vivo-rnai/invivofectamine-reagent.html).

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

Should I use siRNA or vectors for in vivo RNAi?

RNAi can be delivered using two different approaches-siRNA synthetic duplexes or siRNA expressed from plasmids or viral vectors (shRNA, miRNAi). siRNA are becoming the method of choice for the fast development of therapeutics. They are easy to use, easy to design, and easy to synthesize. siRNA can be rapidly identified and multiple genes can be targeted at the same time. With RNAi vectors, the expression will be steadier as a result of the possibility of stable integration of the plasmid into the genome, and they have the ability to target nondividing cells such as stem cells, lymphocytes and neurons. The drawbacks are the danger of oncogenic transformation from insertional mutagenesis, and unanticipated toxicity from long-term silencing of human genes and/or having high amounts of siRNA inside the cell (Grimm D. et al.: Nature 441: 537-541 (2006)).

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

I am planning on using siRNA for my in vivo experiments, what purity should they be?

For Stealth siRNA we recommend to select HPLC in vivo purity. For Invitrogen In Vivo siRNA, we recommend in vivo ready purity.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

Should I use labeled or unlabeled siRNA for my in vivo siRNA experiments?

We have demonstrated that labeling Stealth and Silencer siRNA does not hamper their knockdown potency. An alternative approach is to mix unlabeled duplexes with labeled control duplexes; this method is more commonly used with in vivo siRNA, and allows progression to clinical research unhindered by questions about the possible effects of a label.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

Should I use chemically modified duplexes for my in vivo siRNA experiments?

Chemically modified siRNA duplexes have a number of advantages over standard siRNA duplexes, including the minimization of off-target effects, enhanced stability, and reduced toxicity. For these reasons, chemically modified siRNA duplexes are recommended for in vivo siRNA experiments. For some pilot experiments, where the only aim is to determine biodistribution, unmodified siRNA and fluorescent controls are useful and slightly more cost-effective.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

Where should I add a fluorescent tag to minimize any negative functional effect on my siRNA?

For any RNAi duplex (miRNA or siRNA) there is a possibility that adding a modification will affect how the antisense strand interacts with RISC and aligns with its mRNA target, thus impacting functionality. For that reason, we recommend that you label the 5' end of the sense strand for siRNA (or passenger strand for miRNA). Since the 5' end of the antisense (guide strand) is what gets recognized by RISC, it is best to add the fluorescent modification to the end of the duplex on the strand that does not interact with RISC. You can also test a labeled vs. non-labeled RNAi duplex to determine if the modification is affecting the siRNA or miRNA functionality.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.

How can I find the GC content for the predesigned Silencer siRNA or Silencer Select siRNA sequences prior to purchase?

Please contact Technical Support at techsupport@thermofisher.com to request the GC content for one of our predesigned Silencer siRNA or Silencer Select siRNA sequences.

Find additional tips, troubleshooting help, and resources within our RNAi Support Center.