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EPISOMAL HUMAN IPSC LINE - FAQs

View additional product information for EPISOMAL HUMAN IPSC LINE - FAQs (A13700)

6 product FAQs found

Are the Gibco Episomal hiPSC Line product numbers A13700 and A13777 the same thing?

Yes, these are the same cells. A13777 is the catalog number to order one vial of cells with one product manual.
Part number A13700 cannot be ordered - this number is meant for Thermo Fisher Scientific internal tracking use only.

Can the Gibco Episomal hiPSC Line be grown feeder-free?

Yes, these cells can be cultures in feeder free conditions using StemPro hESC SFM and Geltrex Matrix.

What is the importance of Gibco Episomal hiPSC Line being "zero-footprint"?

Traditional reprogramming methods, such as lentiviral methods, integrate viral DNA into the iPSC genome. This viral DNA insertion can interrupt key genes and is passed on to the daughter cells where it remains part of the DNA of the new iPSC line. Gibco Episomal hiPSC Line does not have any permanent viral DNA integration (zero-footprint).

How was the Gibco Episomal hiPSC Line created?

The Gibco Episomal hiPSC Line is a non-viral, non-integrated line that was generated using cord blood-derived CD34+ progenitors with seven episomally expressed factors (Oct4, Sox2, Klf4, c-Myc, Nanog, Lin28, and SV40 T).

What passage number are the Gibco Episomal hiPSC Line?

The cells were originally launched as passage 26. The passage number of each lot is listed on the Certificate of Analysis.

Is it normal for the Gibco Episomal hiPSC Line to grow slowly when first thawed?

Yes, the cells are slow when first thawed but their doubling time will decrease with time. You should have at least 3 visible colonies by day 8.