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Phospho-CREB pSer129 / pSer133 Antibody (44-297G) in ChIP
ChIP- qPCR analysis of CREB [pSer133] was performed with 10µl of the CREB [pSer133] Rabbit polyclonal antibody (44297G) on sheared chromatin from 2 million HeLa cells treated with 50ng/ml of TNFalpha for one hour using the MAGnify? Chromatin Immunoprecipitation System (49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA from each ChIP sample was analyzed by StepOnePlus? Real-Time PCR System (4376600) with primers for the promoter of active YWHAZ, I-kB gene, used as positive control target, and the inactive GAPDH, used as negative control target. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.
![ChIP- qPCR analysis of CREB [pSer133] was performed with 10µl of the CREB [pSer133] Rabbit polyclonal antibody (44297G) on sheared chromatin from 2 million HeLa cells treated with 50ng/ml of TNFalpha for one hour using the MAGnify? Chromatin Immunoprecipitation System (49-2024). Normal Rabbit IgG was used as a negative IP control. The purified DNA from each ChIP sample was analyzed by StepOnePlus? Real-Time PCR System (4376600) with primers for the promoter of active YWHAZ, I-kB gene, used as positive control target, and the inactive GAPDH, used as negative control target. Data is presented as fold enrichment of the antibody signal versus the negative control IgG using the comparative CT method.](http://tools.thermofisher.com/content/sfs/gallery/low/44297G-CREB-pSpS129-133-ChIP_20150706154133.jpg)
