HPLC Protein Analysis

Analytical protein chromatography solutions for high-performance characterization and quantification

Analytical protein chromatography is a technique used to analyze and characterize proteins by separating them according to their physical and chemical attributes, including size, charge, and affinity. This approach enables the assessment of protein purity, quantification, and identification of alterations such as post-translational modifications (PTMs). Typical analytical methods encompass affinity chromatography, ion exchange chromatography, reversed-phase high-performance liquid chromatography (r-pHPLC), and size-exclusion chromatography.

Our portfolio of Thermo Scientific high-performance liquid chromatography (HPLC) solutions features POROS and CaptureSelect chemistries—widely used in bioprocessing to monitor monoclonal antibody titer and product yield from cell culture supernatant. POROS analytical chromatography columns enable rapid separation of biomolecules for analytical applications, while POROS CaptureSelect analytical columns help expand the advantages of high-speed, high-performance quantitation to antibody fragments and isotypes, biosimilars, and fusion proteins. Analytical columns can be operated on any HPLC or medium-pressure chromatography system.

mAb affinity columns

Discover our specialized HPLC columns designed for the high-resolution separation and analysis of monoclonal antibodies based on their affinity interactions.

Protein affinity columns

Find HPLC columns designed to separate and analyze proteins based on their specific binding interactions with ligands immobilized on the column matrix.
 

General purpose HPLC columns

Explore general-purpose analytical columns, including ion exchange, reverse phase, and activated types, for versatile separation and analysis of diverse chemical compounds.

Analytical chromatography

Understand how protein analytics using high-performance liquid chromatography supports your bioproduction workflow.
 

High-powered POROS and CaptureSelect chemistries

Discover the advantages of our leading-edge chromatography chemistries for efficient protein analysis.

Resources to keep you in the know

Visit the Analytics Knowledge Hub to discover a robust offering of technical content to further your pharma analytics understanding.
 

Additional bioprocessing resources

Discover areas where you can cut costs and gain efficiency using our integrated calculator.

Explore our collection of information and insights to support your drug development journey.

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For Research Use Only. Not for use in diagnostic procedures.