Uneven background stain removed by the Silver Stain Rescue Reagent

Dilutions of an unstained protein molecular weight marker were separated by SDS-PAGE using 4-12% gradient gels and stained with Thermo Scientific Pierce Silver Stain Kit (Part No. 24612). Rescue Reagent working solution for low/non-uniform background (0.1mL reagent A, 0.1mL Reagent B in 50mL water) was used. Stain removal was terminated after 55 minutes.