Comparison of purification of proteins expressed by the 1-Step CHO High-Yield IVT Kit and 1-Step Human High-Yield IVT Kit

GST-Streptokinase (SK) and GST- Erythropoietin (EPO) purified using glutathione agarose as shown in the schematic and eluted with 50 mM glutathione (left panel) were run on SDS-PAGE and stained with Coomassiee stain. On the right panel, GST-Retinoblastoma1 (Rb1) and GST-SK proteins were first bound to glutathione column and then on-column cleaved using HRV3C protease as shown in the schematic to elute pure proteins devoid of the tag.

GST-Streptokinase (SK) and GST- Erythropoietin (EPO) purified using glutathione agarose as shown in the schematic and eluted with 50 mM glutathione (left panel) were run on SDS-PAGE and stained with Coomassiee stain. On the right panel, GST-Retinoblastoma1 (Rb1) and GST-SK proteins were first bound to glutathione column and then on-column cleaved using HRV3C protease as shown in the schematic to elute pure proteins devoid of the tag.

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