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This is the fifth episode of our foundational series, Back to Basics. It will cover early methods for studying HLA, how complement-dependent cytotoxicity (CDC) was used for multiple applications, and the development of increasingly sensitive immunologic and genetic assays for HLA. Technologies highlighted in our rapid tour through history include ELISA, solid-phase bead microarray assays, flow cytometry, SSP, SSO, SBT, and NGS. A key takeaway is the functional significance of earlier assays and how understanding them provides insight into the HLA system and current methodologies.
Instructor:
Linda Olsen, MPH, CHS(ACHI), MLS(ASCP)CM,
Sr. Technical Training Specialist – Molecular, Thermo Fisher Scientific
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