O-glycosylation is a difficult post-translational modification to analyze. The heterogeneity of O-glycosylation can make MS analysis difficult. Currently, there is a growing interest in this type of glycosylation and a need for new tools. The OglyZOR enzyme is an O-glycosidase able to remove O-glycans from proteins under native conditions. Paired with PNGase F, a total deglycosylation of complex biopharmaceuticals can be achieved by middle-level MS.
OpeRATOR is protease that binds to O-glycosylated proteins and digests the protein backbone N-terminally to the glycosylated serine or threonine residues. OpeRATOR can be used to generate O-glycopeptides which can be analyzed by LC-MS for in-depth mapping of O-glycosylation sites and quantitative comparisons of O-glycosylation patterns in biopharmaceuticals.