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Quantitation using TMT isobaric tags is a powerful quantitative workflow that results in high throughput protein analysis at very low levels. Advances have been made across the entire proteomics workflow, most notably the development of the Thermo Scientific TMTpro 16plex Labeling Reagent Kit combined with state-of-the-art advancements in analytical mass spectrometry, that have enabled more accurate, high-throughput quantitative proteomics.
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Isobaric chemical tags, such as TMT tags, are used to quantify relative protein abundance changes in complex protein samples across multiple experimental conditions.