Digital PCR as Part of a Complete Genomics Workflow

Title: Aligning qPCR, dPCR, and NGS to unify and broaden the quantification revolution

Speaker: Paul Hung, PhD, Sr Director and General Manager

Location: Hyatt Regency Atlanta, Regency Ballroom 6

Time: Wednesday, September 29^th, 7–8 AM EST

Abstract: PCR technology laid the foundation for the modern and sophisticated methods of nucleic acid detection, quantification, and interrogation. Quantitative PCR (qPCR) and next-generation sequencing (NGS) are among the most used molecular techniques for these purposes, and recent developments in digital PCR (dPCR) technology, which facilitate highly sensitive and precise absolute quantification of nucleic acids, has accelerated its adoption in the field. Although typically compared and contrasted in terms of dynamic range, precision, and accuracy, qPCR, NGS, and dPCR technologies are all positioned to answer a different breadth of molecular questions. In this session, we will explore the benefits of each technology and how together each strength can be leveraged to tell a more compelling and complete molecular story.