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Delivery Solutions for Immune Cells to Enable and Advance Cell-Based Therapy

Presented by Sean Chang, Ph.D, Cell Biology Research and Development Scientist, Thermo Fisher Scientific

Date: Saturday, May 5, 2018

Presentation: 10:00 – 10:45 am

Room: 1

Abstract: Genetic manipulation of blood cells is key to understanding and developing treatments for a broad range of diseases such as cancers and autoimmune diseases. However, the difficulty of delivering molecules into these cells has been an obstacle to more rapid advancement. Here we present viral and non-viral delivery solutions for immune cells.

The Gibco LV-MAX Lentiviral Production System is the first system that provides a scalable and high-yield lentiviral vector production on a serum-free suspension platform, which facilitates the research from bench to clinic. Additionally, the Invitrogen Neon Transfection System offers non-viral delivery of DNA, RNA, and protein efficiently into a variety of blood cells, including human primary T cells, and achieves more than 90% knockout efficiency with CRISPR/Cas9 RNP delivery. More importantly, Neon provides highly efficient CRISPR/Cas9 gene knock-in with our newly developed all-in-one electroporation method, which is ideal for next generation cell therapy.


New Strategies for Cell-Based Immune Assay Design and Immunotherapy Monitoring

Presented by Chris Langsdorf, Staff Scientist, Thermo Fisher Scientific

Date: Sunday, May 6, 2018

Presentation: 1:45 – 2:30 pm

Location: Room 2

Abstract: With continued growth in development and approval of biologic drugs and cell therapies, comes a need for more robust and predictive cell-based assays. Join us for a discussion of a broad range of novel functional reagents and demonstration of their use in cell-based immunotherapy models using powerful cytometric instrumentation. High-throughput data acquisition streamlines complex experiments while simple protocols for multiplexed analysis provide deeper insight into cell behavior.

Flow cytometry and high content analysis experimental data will be presented that demonstrates performance in receptor occupancy, antibody internalization, and effector function assays using antibodies against CD20, CD71, and HER2 in breast cancer and leukemia models. This workshop will provide valuable additions to your portfolio of assays for immunology research.

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 I will be attending Delivery Solutions for Immune Cells to Enable and Advance Cell-Based Therapy.
 I will be attending New Strategies for Cell-Based Immune Assay Design and Immunotherapy Monitoring.