Preimplantation genetic testing – aneuploidy (PGT-A) is a rapidly growing area of fertility research commonly incorporated into IVF research workflows. PGT-A findings can determine chromosome presence and frequency in embryo biopsies, which provides scientific insight into reducing the time to pregnancy and decreasing miscarriage rates. Ion ReproSeq PGS kits for PGT-A leverage the speed and reliability of Ion Torrent next-generation sequencing (NGS) to offer important insight for future reproductive decisions.
Versatile and comprehensive—detect whole-chromosome, mosaic, and small copy number events, from just 6 pg of DNA from single or multiple cells
Scalable throughput—three kit configurations enable analysis of 16, 24, or 96 samples in a single run on a single platform
Rapid, end-to-end NGS workflow—cells to results in less than 10 hours* and minimized user error, with <2 hours* hands-on time
Enhanced interpretation of results with Ion Reporter Software—mosaicism detection, gender masking, and improved data plotting (for easier data interpretation)
Application note: Detection of aneuploidies, segmental aneuploidies, and mosaicism using Ion ReproSeq kits
ReproSeq PGS kits for reliable and rapid aneuploidy detection
Catherine Welch, MBA, TS (ABB)
Founder and Managing Partner of Sequence46
PGT-A simplified -- Comparison of leading
NGS solutions: Ion ReproSeq
PGS and VeriSeq PGS
Director of Preimplantation Genetics, NxGen MDx
Ion ReproSeq PGS kits include all materials for library construction, template preparation, and sequencing on the Ion GeneStudio S5 System, for cell to analysis in less than 10 hours*. Simple cartridge-loaded reagents, the straightforward user interface of the Ion GeneStudio S5 System, and automated template preparation on the Ion Chef System minimize user errors and facilitate rapid implementation of PGT research into your lab.
The Ion sequencing chips (Ion 510 Chip, Ion 520 Chip, and Ion 530 Chip) provide sample throughput flexibility and scalability to match your needs and enable cost-effective analysis of 16, 24, or 96 samples in a single sequencing run.
*Turnaround time for the Ion ReproSeq PGS Kit with Ion 510 Chips (16 samples/run) is less than 10 hours with less than 2 hours of hands on time
Combine the PGD-SEQ kit with Ion ReproSeq PGS kit using the Ion GeneStudio S5 System for a simple, scalable and automated sample-to-answer workflow solution that includes PGT-M and PGT-A results from a single research sample.
Torrent Suite Software coordinates all of the experiment planning and data processing necessary to complete your targeted sequencing workflow. The intuitive, web-based Torrent Browser enables users to plan, monitor, and view sequencing run results quickly and simply. Data generated on an Ion GeneStudio S5 System is first transferred to the Ion S5 Torrent Server for base calling, then automatically uploaded to Ion Reporter Software for further analysis.
The aneuploidy analysis workflow in Ion Reporter Software makes analyzing and reporting aneuploidy results simple and fast. This low-pass coverage analysis workflow processes samples by comparing them to a built-in bioinformatics control baseline to deliver the DNA ploidy status of each sample. Users can visualize aneuploidy profiles using the customized IGV Karyotype View and create their own interpretive report.
Figure 1. Aneuploidy detection from a single cell using the Ion ReproSeq PGS kits. Data shown in the top panel illustrate a deviation (in blue) from baseline, indicating 45 Mbp duplication of chromosome 9 (male). Data shown on bottom panel indicate a deletion (in red) of 48 Mbp on chromosome 13 (female).
For Research Use Only. Not for use in diagnostic procedures.