Passive Binding Surfaces—Solid Phase Guide
A wide variety of surfaces are available for performance optimization
Passive surfaces have a broad range of applications as they can bind to a variety of biomolecules. Passive binding is primarily suited for the immobilization of medium- to large-sized molecules, such as antibodies, which are capable of establishing several contact points. The exact molecular interaction sites are dependent on the specific matching of the biomolecule's structure with the polymer surface structure. A large variety of biomolecules can be immobilized on passive surfaces with good residual activity.
Your dedicated partner for In Vitro Diagnostics.
Clear Immuno Plates
- Thermo Scientific™ 96-Well Microtiter™ Microplates
- Clear C-Shaped Immuno Nonsterile 96-Well Plates
- Clear Flat-Bottom Immuno Nonsterile 384-Well Plates
- Clear Flat-Bottom Immuno Nonsterile 96-Well Plates
- Clear Flat-Bottom Immuno Sterile 96-Well Plates
- Clear Round-Bottom Immuno Nonsterile 96-Well Plates
Passive Binding Surface Categories
These surfaces are typically used for the adsorption of hydrophobic molecules such as lipid rich biomolecules.
The slight hydrophilicity of these plates enhances their ability to bind a diverse range of biomolecules, including glycoproteins, serum-containing samples and amphoteric molecules such as lipopolysaccarides. With these plates, non-specific adsorption of serum-containing samples are reduced, and this will improve the signal-to-noise (S/N) ratio and consequently, sensitivity levels.
Optimized to bind high amounts of IgG (polyclonal), these plates are ideal for antibody sandwich assays (e.g., ELISAs). In addition, they show increased binding of many other proteins and biomolecules that possess hydrophilic/hydrophobic characteristics.
The most hydrophilic in our portfolio, many hydrophilic proteins will bind with a high affinity to these plates. Binding does, however, tend to be more pH sensitive.