Technical Resources

21010 - Basal Medium Eagle (BME), no Glutamine

Components Molecular Weight Concentration (mg/L) mM
Amino Acids
L-Arginine hydrochloride 211.0 21.0 0.09952607
L-Cystine 2HCl 313.0 16.0 0.05111821
L-Histidine 155.0 8.0 0.051612902
L-Isoleucine 131.0 26.0 0.19847329
L-Leucine 131.0 26.0 0.19847329
L-Lysine hydrochloride 183.0 36.47 0.19928962
L-Methionine 149.0 7.5 0.05033557
L-Phenylalanine 165.0 16.5 0.1
L-Threonine 119.0 24.0 0.20168068
L-Tryptophan 204.0 4.0 0.019607844
L-Tyrosine disodium salt dihydrate 261.0 26.0 0.099616855
L-Valine 117.0 23.5 0.2008547
Vitamins
Biotin 244.0 1.0 0.0040983604
Choline chloride 140.0 1.0 0.007142857
D-Calcium pantothenate 477.0 1.0 0.002096436
Folic Acid 441.0 1.0 0.0022675737
Nicotinamide 122.0 1.0 0.008196721
Pyridoxal hydrochloride 204.0 1.0 0.004901961
Riboflavin 376.0 0.1 2.6595744E-4
Thiamine hydrochloride 337.0 1.0 0.002967359
i-Inositol 180.0 2.0 0.011111111
Inorganic Salts
Calcium Chloride (CaCl2) (anhyd.) 111.0 200.0 1.8018018
Magnesium Sulfate (MgSO4) (anhyd.) 120.0 97.7 0.81416667
Potassium Chloride (KCl) 75.0 400.0 5.3333335
Sodium Bicarbonate (NaHCO3) 84.0 2200.0 26.190475
Sodium Chloride (NaCl) 58.0 6800.0 117.24138
Sodium Phosphate monobasic (NaH2PO4-H2O) 138.0 140.0 1.0144928
Other Components
D-Glucose (Dextrose) 180.0 1000.0 5.5555553
Phenol Red 376.4 10.0 0.026567481

Reference:

1. Eagle, H. (1965) Proc. Exp. Biol. Med., 89:362.

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41010 - Basal Medium Eagle (BME), no Glutamine
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