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Fibronectin Antibody (MIF2601) in ELISA
Direct ELISA analysis of Ferritin was performed by coating wells of a 96-well plate with 100ul per well of Human Ferritin or BSA diluted in carbonate/bicarbonate buffer (Product ## 28382), starting at a concentration of 1ug/ml and serially diluting 2-fold to a concentration of 2ng/ml, overnight at 4C. Wells of the plate were washed, blocked with StartingBlock blocking buffer (Product ## 37538), and incubated with 100ul per well of a mouse anti-ferritin monoclonal antibody (Product ## MIF2601) at a concentration of 1ug/ml for 1 hour at room temperature. The plate was washed, then incubated with 100ul per well of an HRP-conjugated goat anti-mouse IgG secondary antibody (Product ## 31430) at a dilution of 1:25000 for 30 minutes at room temperature. Detection was performed using 1-Step Ultra TMB substrate (Product ## 34028) for 10 minutes at room temperature in the dark. The reaction was stopped with 0.16M sulfuric acid, and absorbances were read on a spectrophotometer at 450-550nm.
