PRDX1 Antibody (LFMA0073) in WB

Western blot analysis was performed on membrane enriched extracts (30 ug lysate) of HeLa (Lane 1), MCF7 (Lane 2), HCT 116 (Lane 3), A549 (Lane 4), A-431 (Lane 5) and Hep G2 (Lane 6). The blot was probed with 2-Cys Peroxiredoxin Mouse monoclonal Antibody (Product# LF-MA0073, 2 ug/ml) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 ug/ml, 1:4000 dilution). A 22 kDa band corresponding to 2-Cys Peroxiredoxin was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

Western blot analysis was performed on membrane enriched extracts (30 ug lysate) of HeLa (Lane 1), MCF7 (Lane 2), HCT 116 (Lane 3), A549 (Lane 4), A-431 (Lane 5) and Hep G2 (Lane 6). The blot was probed with 2-Cys Peroxiredoxin Mouse monoclonal Antibody (Product# LF-MA0073, 2 ug/ml) and detected by chemiluminescence using Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A28177, 0.25 ug/ml, 1:4000 dilution). A 22 kDa band corresponding to 2-Cys Peroxiredoxin was observed across the cell lines tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 4-12 % Bis-Tris gel (Product # NP0322BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with iBlot® 2 Dry Blotting System (Product # IB21001). The membrane was probed with the relevant primary and secondary Antibody following blocking with 5% skimmed milk. Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

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