TGF beta-1 Antibody (MA1-116) in WB

Western blot analysis of human TGF beta-1 was performed by loading 10ul of human serum from patients with multiple sclerosis (MS), leukemia, or asthma (left panel) and 2ug and 1ug of recombinant human TGF beta-1 (right panel) protein per well on a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with StartingBlock (TBS) Blocking Buffer (Product ## 37542) for at least 1 hour. The membrane was probed with a TGF beta-1 monoclonal antibody (Product ## MA1-116) at a concentration of 5ug/ml overnight at 4C on a rocking platform, washed in TBS-0.1%Tween-20, and probed with a goat anti-mouse IgG-HRP secondary antibody (Product ## 31430) at a dilution of 1:10,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura (Product ## 34075).

Western blot analysis of human TGF beta-1 was performed by loading 10ul of human serum from patients with multiple sclerosis (MS), leukemia, or asthma (left panel) and 2ug and 1ug of recombinant human TGF beta-1 (right panel) protein per well on a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked with StartingBlock (TBS) Blocking Buffer (Product ## 37542) for at least 1 hour. The membrane was probed with a TGF beta-1 monoclonal antibody (Product ## MA1-116) at a concentration of 5ug/ml overnight at 4C on a rocking platform, washed in TBS-0.1%Tween-20, and probed with a goat anti-mouse IgG-HRP secondary antibody (Product ## 31430) at a dilution of 1:10,000 for at least 1 hour. Chemiluminescent detection was performed using SuperSignal West Dura (Product ## 34075).

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