Phospho-ZAP70/Syk (Tyr319, Tyr352) Antibody (17900642) in Flow

LEFT: Intracellular staining of whole human blood left unstimulated (orange histogram) or stimulated with Anti-Human CD3 Functional Grade Purified (Product # 16-0038) and goat anti-mouse IgG for 5 minutes (purple histogram) with Anti-Human/Mouse phospho-ZAP-70/SYK (Y319/Y352) APC. The 1-step Fix/Lyse Solution (Product # 00-5333) and Permeabilization Buffer (Product # 00-8333) and protocols were used for staining. Lymphocytes in the CD3+ gate were used for analysis. RIGHT: Intracellular staining of untreated (orange histogram) or 5-minute hydrogen peroxide-activated sodium pervanadate-treated (purple histogram) mouse spleen cells with Anti-Human/Mouse phospho-ZAP-70/SYK (Y319/Y352) APC using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824) and protocol. Lymphocytes in the CD3+ gate were used for analysis.

LEFT: Intracellular staining of whole human blood left unstimulated (orange histogram) or stimulated with Anti-Human CD3 Functional Grade Purified (Product # 16-0038) and goat anti-mouse IgG for 5 minutes (purple histogram) with Anti-Human/Mouse phospho-ZAP-70/SYK (Y319/Y352) APC. The 1-step Fix/Lyse Solution (Product # 00-5333) and Permeabilization Buffer (Product # 00-8333) and protocols were used for staining. Lymphocytes in the CD3+ gate were used for analysis. RIGHT: Intracellular staining of untreated (orange histogram) or 5-minute hydrogen peroxide-activated sodium pervanadate-treated (purple histogram) mouse spleen cells with Anti-Human/Mouse phospho-ZAP-70/SYK (Y319/Y352) APC using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824) and protocol. Lymphocytes in the CD3+ gate were used for analysis.

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