PARP Antibody (436400) in WB

Knockdown of PARP was achieved by transfecting HeLa cells with PARP specific validated siRNA (Silencer® select Product ## s1097). Western blot analysis (Fig a) was performed using whole cell lysates from the PARP knock down cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with Anti-PARP Mouse Monoclonal Antibody (Product ## 436400, 1-2 µg/mL) and Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP conjugate (Product ## 626520, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram(Fig b). Loss of signal upon siRNA mediated knock down confirms that antibody is specific to PARP.

Knockdown of PARP was achieved by transfecting HeLa cells with PARP specific validated siRNA (Silencer® select Product ## s1097). Western blot analysis (Fig a) was performed using whole cell lysates from the PARP knock down cells (lane 3), non-specific scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blots were probed with Anti-PARP Mouse Monoclonal Antibody (Product ## 436400, 1-2 µg/mL) and Goat anti-Mouse IgG (H+L) Secondary Antibody, HRP conjugate (Product ## 626520, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram(Fig b). Loss of signal upon siRNA mediated knock down confirms that antibody is specific to PARP.

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