HeLa cells imaged with B-catenin antibody and goat anti-rabbit Qdot® 655
HeLa cells were plated on coverslips overnight. The next day the cells were fixed and permeabilized using the Image-iT® Fixation/Permeabilization Kit (Cat. No. R37602) according to protocol. Cells were then incubated with 3 µg/mL anti-B-catenin (Cat. No. 712700) for 60 mins at RT followed by 3 wash steps in dPBS. Cells were then incubated with a 1:1000 dilution of goat anti-rabbit Qdot® 655 (Cat. No. Q11422MP) secondary antibody for 60 mins followed by 3 wash steps in dPBS. Cells were labeled with ReadyProbes® reagents NucBlue® Live (Cat. No. R37605) and ActinRed™ 555 (Cat. No. R37112) according to protocol. Coverslips were then mounted using ProLong® Gold Antifade Reagent (Cat. No. P36930). Images were taken using the EVOS® FL Auto Imaging System and a 40X coverslip-corrected objective (Cat. No. AMEP-4699).