Linear Detection Range of Qubit®/Quant-iT™ RNA Assay

The Qubit®/Quant-iT™ RNA Assay has a linear detection range of 5–100 ng in sample volumes of 1–20 µL and is selective for RNA, even in the presence of an equal mass of DNA. The x-axis gives the mass of nucleic acid at a given point when DNA or RNA is assayed alone. In the 1:1 mixture, the total mass of nucleic acid at a given point is double what is stated on the x-axis.