RNF174 (MARCH4) is a multi-pass membrane protein mainly found in the membrane of Golgi apparatus. It is a complex enzyme with a RING-CH-type zinc finger. It acts as a ubiquitin-protein ligase mainly involved in third step of ubiquitin conjugation. The RINGv-type zinc finger domain is required for E3 ligase activity in which the E3 ubiquitin-protein ligase mediates ubiquitination of MHC-I and CD4, and promote their subsequent endocytosis and sorting to lysosomes via multivesicular bodies. It is mainly expressed in brain and placenta.
E3 ubiquitin-protein ligase MARCH4; MARCH-IV; membrane associated ring finger 4; membrane-associated ring finger (C3HC4) 4, E3 ubiquitin protein ligase; Membrane-associated RING finger protein 4; Membrane-associated RING-CH protein IV; RING finger protein 174; RING-type E3 ubiquitin transferase MARCH4; RNF174