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Invitrogen

CD117 (c-Kit) Monoclonal Antibody (YB5.B8), PE, eBioscience™

5 Published Figures
11 References
View all (207) c-Kit (CD117) antibodies

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Product Details

12-1179-42

Applications
Tested Dilution
Publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test
View 11 publications 11 publications
Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

YB5.B8

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1659666

Product Specific Information

Description: The YB5.B8 monoclonal antibody reacts with human CD117, also known as c-Kit, Steel factor receptor and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145 kDa molecule is expressed by hematopoietic progenitor cell subsets and mast cells. The interaction of c-Kit and Steel factor promotes proliferation and differentiation of hematopoietic progenitor cells and mast cell differentiation. CD117 is also expressed by melanocytes and plays a role in signaling and activation of these cells.

When interested in staining HSCs from peripheral blood, we recommend the use of clone 104D2. Please refer to Product # 17-1178.

Applications Reported: YB5.B8 has been reported for use in flow cytometric analysis.

Applications Tested: The YB5.B8 antibody has been tested by flow cytometric analysis of the TF-1 cell line, a human erythroleukemia cell line. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

KIT (c-KIT) is a proto-oncogene and a type 3 transmembrane receptor for MGF (mast cell growth factor, also known as stem cell factor). KIT was first identified as the cellular homolog of the feline sarcoma viral oncogene v-kit. KIT together with its ligand regulates growth and activation of a variety of hemopoietic and non-hemopoietic cells. Mutations in KIT are associated with gastrointestinal stromal tumors, mast cell disease, acute myelogenous leukemia, and piebaldism. Recently, deregulation of the KIT receptor TK by the prevalent activation loop mutation D816V has served as a focal point in therapeutic strategies aimed at curbing neoplastic mast cell growth. c-Kit is expressed in hematopoietic stem cells, germ cells, mast cells and gastrointestinal tract cajal cells. Upon binding of its ligand stem cell factor (SCF), c-kit dimerizes, resulting in receptor activation and autophosphorylation of various tyrosine residues including tyrosine 703 located on the cytoplasmic domain of the receptor. This modification allows docking of Grb2 and activation of the Ras/ERK signaling pathway. SCF/c-kit can activate multiple downstream signaling pathways including PI3K, PLC-gamma and JAK/STAT. c-kit receptor activation is essential for hematopoiesis, stem cell maintenance and gametogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Bioinformatics

Protein Aliases: c-Kit protooncogene; CD117; ckit; Mast/stem cell growth factor receptor Kit; p145 c-kit; PBT; Piebald trait protein; protein kinase; Proto-oncogene c-Kit; proto-oncogene tyrosine-protein kinase Kit; SCFR; Tyrosine-protein kinase Kit; unnamed protein product; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog; v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene-like protein

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Gene Aliases: C-Kit; CD117; KIT; MASTC; PBT; SCFR

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UniProt ID: (Human) P10721

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Entrez Gene ID: (Human) 3815

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Function(s)
nucleotide binding protease binding protein kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity stem cell factor receptor activity protein binding ATP binding kinase activity transferase activity growth factor binding cytokine binding SH2 domain binding protein homodimerization activity metal ion binding
Process(es)
ovarian follicle development hematopoietic progenitor cell differentiation myeloid progenitor cell differentiation lymphoid progenitor cell differentiation immature B cell differentiation mast cell chemotaxis positive regulation of cytokine production involved in immune response positive regulation of dendritic cell cytokine production glycosphingolipid metabolic process chemotaxis inflammatory response signal transduction transmembrane receptor protein tyrosine kinase signaling pathway spermatogenesis spermatid development cell proliferation positive regulation of cell proliferation germ cell migration regulation of cell shape gonad development visual learning male gonad development response to radiation positive regulation of gene expression programmed cell death cell migration cytokine-mediated signaling pathway stem cell population maintenance lamellipodium assembly actin cytoskeleton organization hemopoiesis B cell differentiation T cell differentiation erythrocyte differentiation melanocyte differentiation positive regulation of cell migration positive regulation of pseudopodium assembly positive regulation of mast cell cytokine production somatic stem cell population maintenance embryonic hemopoiesis ectopic germ cell programmed cell death intracellular signal transduction hematopoietic stem cell migration megakaryocyte development Fc receptor signaling pathway Kit signaling pathway erythropoietin-mediated signaling pathway regulation of cell proliferation positive regulation of tyrosine phosphorylation of STAT protein negative regulation of programmed cell death mast cell degranulation positive regulation of MAPK cascade pigmentation tongue development leukocyte activation positive regulation of Notch signaling pathway positive regulation of JAK-STAT cascade response to cadmium ion protein autophosphorylation developmental pigmentation positive regulation of long-term neuronal synaptic plasticity digestive tract development stem cell differentiation epithelial cell proliferation regulation of developmental process detection of mechanical stimulus involved in sensory perception of sound positive regulation of sequence-specific DNA binding transcription factor activity negative regulation of developmental process positive regulation of protein kinase B signaling cell chemotaxis mast cell differentiation mast cell proliferation positive regulation of mast cell proliferation melanocyte migration melanocyte adhesion positive regulation of pyloric antrum smooth muscle contraction regulation of bile acid metabolic process positive regulation of colon smooth muscle contraction positive regulation of small intestine smooth muscle contraction positive regulation of vascular smooth muscle cell differentiation negative regulation of reproductive process
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