Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Invitrogen
Description: The monoclonal antibody 135.4 recognizes ILT1, also known as CD85h, LIR7 and LILRA2. ILT1 is a member of the large family of ILT molecules (Immunologulin-like Transcripts) also known as Leukocyte Ig-like Receptors (LIRs) and Monocyte/Macrophage Ig-like Receptors (MIRs). ILT1 contains a short cytoplasmic domain. Through the cytoplasmic domain ILT1 associates with the Fc receptor gamma chain but not DAP12 resulting in a stimulatory signal. ILT1 may act as a receptor for MHC Class I antigens. Expression of ILT1 is mainly found on monocytes, macrophages, DC and weakly on granulocytes. ILT1 is thought to play a role in the TH1/Th2 balance as well as TLR signaling. The monoclonal antibody 135.4 has been reported to have activating properties upon crosslinking which result in calcium influx.
Applications Reported: This 135.4 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This 135.4 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Leukocyte Ig-like receptors (LIRs) are a family of immunoreceptors expressed predominantly on monocytes and B cells and at lower levels on dendritic cells and natural killer (NK) cells. All LIRs in subfamily B have an inhibitory function (LILRB1; MIM 604811). LIRs in subfamily A, with short cytoplasmic domains lacking an immunoreceptor tyrosine-based inhibitory motif (ITIM) and with trans membrane regions containing a charged arginine residue, may initiate stimulatory cascades. One member of subfamily A (LILRA3; MIM 604818) lacks a transmembrane region and is presumed to be a soluble receptor.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Protein Aliases: CD85 antigen-like family member H; CD85h; ILT-1; Immunoglobulin-like transcript 1; leucocyte Ig-like receptor A2; Leukocyte immunoglobulin-like receptor 7; Leukocyte immunoglobulin-like receptor subfamily A member 2; leukocyte immunoglobulin-like receptor subfamily A member 2 soluble; leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 2; LIR-7
Gene Aliases: CD85H; ILT1; LILRA2; LIR-7; LIR7
UniProt ID: (Human) Q8N149
Entrez Gene ID: (Human) 11027
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