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ROR gamma (t) Monoclonal Antibody (AFKJS-9), PE, eBioscience™
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FIGURE: 1 / 19
ROR gamma (t) Antibody (12-6988-82) in Flow
Surface staining of mouse thymocytes with Anti-Mouse CD4 FITC (Product # 11-0041-82) and Anti-Mouse CD8a APC (Product # 17-0081-82), followed by intracellular staining with 0.25 µg of Rat IgG2a K Isotype Control PE (Product # 12-4321-80) (left) or 0.25 µg of Anti-Human/Mouse ROR gamma (t) PE (right) using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. The histograms demonstrate staining of CD4+CD8+ double-positive cells (blue histogram) or CD4+CD8- single-positive cells (purple histogram).
Product Details
12-6988-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rhesus monkey
Published species
Baboon,
Human,
Mouse,
Pig,
Rhesus monkey
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
AFKJS-9
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The AFKJS-9 monoclonal antibody reacts with the mouse, human and rhesus monkey ROR gamma (t) protein. ROR gamma is a member of the retinoic acid-related orphan receptor (ROR) family, which also includes ROR alpha and ROR beta. ROR family proteins are ligand-dependent transcription factors that play roles in multiple physiological processes. ROR gamma is expressed in several tissues including liver, lung, muscle, heart and kidney. Furthermore, it was discovered that alternative transcription results in the expression of an isoform, ROR gamma (t), which is expressed exclusively in cells of the lymphoid compartment, namely CD4+CD8+ "double-positive" thymocytes, Th17 cells of the periphery and lymphoid tissue inducer (LTi) cells of lymphoid organs.
The ROR gamma (t) isoform differs from ROR gamma by three unique amino acids at its amino terminus. Therefore, the AFKJS-9 antibody will react with both the ROR gamma and ROR gamma (t) isoforms.
Applications Reported: This AFKJS-9 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This AFKJS-9 antibody has been tested by intracellular staining followed by flow cytometric analysis of mouse thymocytes and Th17-polarized splenocytes using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to BestProtocols®: Protocol B: One step protocol for (nuclear) intracellular proteins located under the Resources Tab online. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
ROR gamma, a NR1 Thyroid Hormone-Like receptor, has been shown to affect thymopoiesis, bone metabolism, T-cell apoptosis, and lymphoid organogenesis. ROR gamma has been shown to promote thymocyte survival by activating the expression of the antiapoptotic protein Bcl-x(L). ROR gamma is also required for the development of lymph nodes and Peyer's patches. It has been shown that ROR gamma(t), a thymus-specific isoform of ROR gamma from mouse, inhibits Fas ligand expression and cytokine secretion in immature thymocytes. ROR gamma binds as a monomer to response elements composed of a single core motif, GGTCA, preceded by a 6 bp AT-rich sequence. ROR gamma expression has been documented in mouse thymus, adipose, bone, skeletal muscle, liver, and kidney, and in human skeletal muscle. ESTs have been isolated from human tissue libraries, including cancerous head/neck, germ cell, and prostate, and normal colon, head/neck, lung, liver/spleen, and pancreas.
ROR gammat t (RORgt) is an isoform of RORC.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: MGC129539; Nuclear receptor ROR-gamma; Nuclear receptor RZR-gamma; Nuclear receptor subfamily 1 group F member 3; RAR-related orphan receptor C; retinoic acid-binding receptor gamma; retinoid-related orphan receptor gamma; Retinoid-related orphan receptor-gamma; ROR Gamma; ROR gamma t; RORC; RORgt; RP11-98D18.11-001; Thymus orphan receptor; TOR; unnamed protein product
Gene Aliases: EGK_01272; IMD42; NR1F3; RORC; RORG; RORgamma; RZR-GAMMA; RZRG; Thor; TOR
UniProt ID: (Human) P51449, (Mouse) P51450
Entrez Gene ID: (Rhesus monkey) 717052, (Human) 6097, (Mouse) 19885
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II transcription factor activity, sequence-specific DNA binding
transcriptional repressor activity, RNA polymerase II transcription regulatory region sequence-specific binding
DNA binding
transcription factor activity, sequence-specific DNA binding
RNA polymerase II transcription factor activity, ligand-activated sequence-specific DNA binding
protein binding
oxysterol binding
zinc ion binding
sequence-specific DNA binding
metal ion binding
transcription factor activity, direct ligand regulated sequence-specific DNA binding
sequence-specific double-stranded DNA binding
transcription coactivator binding
steroid hormone receptor activity
C4 zinc finger nuclear receptor
negative regulation of transcription from RNA polymerase II promoter
regulation of transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
xenobiotic metabolic process
circadian rhythm
regulation of gene expression
regulation of glucose metabolic process
regulation of steroid metabolic process
intracellular receptor signaling pathway
circadian regulation of gene expression
cellular response to sterol
T-helper cell differentiation
positive regulation of circadian rhythm
regulation of fat cell differentiation
positive regulation of transcription, DNA-templated
rhythmic process
lymph node development
Peyer's patch development
adipose tissue development
negative regulation of thymocyte apoptotic process
T-helper 17 cell differentiation
transcription, DNA-templated
protein phosphorylation
multicellular organism development
cell differentiation
interleukin-17 production
T cell differentiation in thymus
steroid hormone mediated signaling pathway
regulation of gamma-delta T cell differentiation
alpha-beta T cell differentiation
mucosal-associated lymphoid tissue development
regulation of transcription involved in cell fate commitment
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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