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CD217 (IL-17Ra) Monoclonal Antibody (PAJ-17R), PE, eBioscience™
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-Flow-20170216130641.jpg?time=20240507 "CD217 (IL-17Ra) Antibody in Flow Cytometry (Flow)")
FIGURE: 1 / 2
CD217 (IL-17Ra) Antibody (12-7182-82) in Flow
Staining of C57BL/6 bone marrow cells with Anti-Mouse Ly-6G (Gr-1) FITC (Product # 11-5931-82) and 0.25 µg of Rat IgG2a kappa Isotype Control PE (Product # 12-4321-80) (left) or 0.25 µg of Anti-Mouse IL-17 Receptor PE (right). Total viable cells were used for analysis.
-Flow-20170216130641.jpg?time=20240507)
-Flow-20170216130641.jpg?time=20240507?time=20240507 "CD217 (IL-17Ra) Antibody (12-7182-82) in Flow")
Product Details
12-7182-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2a
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
PAJ-17R
Conjugate
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The monoclonal antibody PAJ-17R recognizes mouse IL-17 receptor A (IL-17RA) also known as CD217. This single transmembrane domain-containing receptor can homo- or heterodimerize with IL-17RC or IL-17RB. IL-17RA binds to IL-17A, IL-17F(cytokines of the Th 17 lineage), or IL-17E (IL-25). Ligand binding to the single subunit of IL-17RA has been shown to induce homo- or heterodimerization of the receptor complex that in turn recruits Act-1 via the SEFIR domains leading to recruitment of TRAF6 and signaling through the NFkappaB and MAPK pathways.
Initial reports suggested ubiquitous expression of IL-17RA based on binding studies with IL-17-Fc and expression of mRNA. Interestingly, despite this, not all cells expressing the receptor respond to IL-17A. In particular, resting T cells do not respond. It is not known if the expression level of IL-17RA protein varies or if some cells express the receptor but are not able to activate downstream signaling events. Recent evidence suggests a more limited expression profile based on IL-17RA knock-out mice which display decreased numbers of neutrophils with impaired function in response to challenge with K. pneumonia or Candida albicans. This suggests IL-17RA plays a more dominant and critical role in neutrophil development and function. Our studies suggest that expression can be found on abundantly on Gr-1^dim population and many of the Gr-1^bright bone marrow cells, as well as some F4/80 positive cells. This staining with PAJ-17R is not detected in IL-17RA knock-out mice.
Applications Reported: This PAJ-17R antibody has been reported for use in flow cytometric analysis.
Applications Tested: This PAJ-17R antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
Interleukin 17A (IL-17A) is a proinflammatory cytokine secreted by activated T-lymphocytes. It is a potent inducer of the maturation of CD34-positive hematopoietic precursors into neutrophils. IL-17RA is a ubiquitous type I membrane glycoprotein that binds with low affinity to IL-17A and IL-17F. IL-17A and its receptor play a pathogenic role in many inflammatory and autoimmune diseases such as rheumatoid arthritis. Defects in IL-17RA are the cause of familial candidiasis type 5 (CANDF5) which is a rare disorder with altered immune responses and impaired clearance of fungal infections.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD127; CD217; IL-17 receptor A; IL-17RA; Interleukin-17 receptor A; MGC10262
Gene Aliases: AW538159; Cdw217; Il17r; Il17ra; VDw217
UniProt ID: (Mouse) Q60943
Entrez Gene ID: (Mouse) 16172
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