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Invitrogen
IL-17AF Monoclonal Antibody (B8KN8R), PE, eBioscience™
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FIGURE: 1 / 2
IL-17AF Antibody (12-9171-82) in Flow
Mouse splenocytes were cultured under Th17-polarizing conditions for 6 days then restimulated with the Cell Stimulation Cocktail (plus protein transport inhibitors) (Product # 00-4975-03) for 5 hours. The cells were then intracellularly stained with Anti-Mouse CD4 FITC (Product # 11-0042-82) and 0.125 µg of Rat IgG2a K Isotype Control PE (Product # 12-4321-80) (left) or 0.125 µg of Anti-Mouse IL-17AF PE (right), using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Viable cells in the lymphocyt... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
Product Details
12-9171-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
B8KN8R
Immunogen
Recombinant IL-17AF
Conjugate
PE
PE
PE
Excitation/Emission Max
565/576 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The B8KN8R monoclonal antibody reacts with mouse interleukin (IL)-17AF heterodimer. This antibody specifically reacts with this heterodimer and has no reactivity for IL-17A or IL-17F homodimers. IL-17A and IL-17F are well-characterized homodimeric cytokines secreted by T helper 17 (Th17) cells, gamma-delta T cells and several subsets of innate lymphoid cells. Somewhat less appreciated, IL-17A and IL-17F subunits can also form the heterodimer, IL-17AF. Together, these three dimers signal through the IL-17RA/IL-17RC receptor complex to mediate immune responses at mucosal interfaces and are found at lesion sites in inflammatory bowel disease, asthma, atopic dermatitis and rheumatoid arthritis.
Applications Reported: This B8KN8R antibody has been reported for use in intracellular staining followed by flow cytometric analysis.
Applications Tested: This B8KN8R antibody has been tested by intracellular staining and flow cytometric analysis of Th17-polarized mouse splenocytes, using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to Best Protocls: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resource Tab online. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
IL-17A and IL-17F are well-characterized homodimeric cytokines secreted by T helper 17 (Th17) cells, gamma-delta T cells and several subsets of innate lymphoid cells. Somewhat less appreciated, IL-17A and IL-17F subunits can also form the heterodimer, IL-17AF. Together, these three dimers signal through the IL-17RA/IL-17RC receptor complex to mediate immune responses at mucosal interfaces and are found at lesion sites in inflammatory bowel disease, asthma, atopic dermatitis and rheumatoid arthritis.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: cytokine; cytokine; IL-17F; cytotoxic T-lymphocyte-associated antigen 8; il 17; ILN; Interleukin; unnamed protein product
Gene Aliases: Ctla-8; Ctla8; IL-17; IL-17A; IL-17F; Il17
UniProt ID: (Mouse) Q62386, (Mouse) Q7TNI7
Entrez Gene ID: (Mouse) 16171, (Mouse) 257630
positive regulation of antimicrobial peptide production
inflammatory response
Notch signaling pathway
response to wounding
gene expression
positive regulation of keratinocyte proliferation
keratinocyte differentiation
positive regulation of interleukin-1 beta production
positive regulation of interleukin-12 production
positive regulation of interleukin-16 production
positive regulation of interleukin-23 production
positive regulation of interleukin-6 production
positive regulation of tumor necrosis factor production
interleukin-17A-mediated signaling pathway
keratinocyte proliferation
positive regulation of osteoclast differentiation
positive regulation of transcription from RNA polymerase II promoter
defense response to Gram-negative bacterium
defense response to Gram-positive bacterium
defense response to fungus
positive regulation of protein kinase B signaling
intestinal epithelial structure maintenance
cellular response to interleukin-1
cellular response to glucocorticoid stimulus
fibroblast activation
interleukin-17-mediated signaling pathway
granulocyte migration
negative regulation of inflammatory response to wounding
positive regulation of cytokine production involved in inflammatory response
positive regulation of bicellular tight junction assembly
negative regulation of interleukin-17-mediated signaling pathway
positive regulation of chemokine (C-X-C motif) ligand 1 production
positive regulation of cytokine production
negative regulation of angiogenesis
regulation of transforming growth factor beta receptor signaling pathway
regulation of granulocyte macrophage colony-stimulating factor production
regulation of interleukin-2 production
regulation of interleukin-6 production
regulation of interleukin-8 production
positive regulation of lymphotoxin A production
cartilage development
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