Invitrogen

CD45 Monoclonal Antibody (2D1), PE, eBioscience™

Advanced Verification

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

13 Published Figures

28 References

View all (396) CD45 antibodies

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Datasheet

Protocols

Questions & Answers

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CD45 Antibody in Flow Cytometry (Flow) — Knockout of CD45 was achieved by CRISPR-Cas9 genome editing using LentiArray™ Lentiviral sgRNA (Product # A32042, Assay ID CRISPR664203_LV) and LentiArray Cas9 Lentivirus (Product # A32064). Flow cytometry analysis of CD45 was performed by staining THP-1 CD45 Knock out cells with 0.25 µg Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™ (Product # 12-4714-82) or 0.25 µg CD45 Monoclonal Antibody (2D1), PE, eBioscience™ (Product # 12-9459-42, blue histogram). THP-1 Cas9 control cells were also stained with0.25 µg CD45 Monoclonal Antibody (2D1), PE, eBioscience™ (Product # 12-9459-42, pink histogram). Lossof signal was observed in the KOcells stained with CD45 antibody clone 2D1 but not in the control Cas9cells. Viable cells were used for analysis, as determined by Fixable Viability DyeeFluor™780 (Product # 65-0865-18).

CD45 Antibody — Antibody clone (2D1) specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. Lossof signal was observed for target protein in 2D1 KOcells (blue histogram) compared to the control Cas9cells (pink histogram) using CD45 antibody (2D1). Yellow histogram represents staining with the isotype control.

Product Details

12-9459-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.25 µg)/test

View 29 publications 29 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

2D1

Conjugate

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

RRID

AB_10718238

Product Specific Information

Description: The 2D1 monoclonal antibody reacts with all isoforms of human CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.

Applications Reported: This 2D1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 2D1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.25 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase (PTP) ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 is absent on non-hematopoietic cell lines, normal and malignant, non-hematopoietic tissues. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing, differ in their extracellular domains but share identical transmembrane and cytoplasmic domains. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45 has been shown to be an essential regulator of T- and B-cell antigen receptor signaling and suppresses JAK kinases to regulate cytokine receptor signaling. CD45 is also important in promoting cell survival by modulating integrin-mediated signal transduction pathway, DNA fragmentation during apoptosis and inhibition or upregulation of various immunological functions.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Flow Cytometry

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Product References

Gasdermin D-dependent platelet pyroptosis exacerbates NET formation and inflammation in severe sepsis.

Nature cardiovascular research

Su M,Chen C,Li S,Li M,Zeng Z,Zhang Y,Xia L,Li X,Zheng D,Lin Q,Fan X,Wen Y,Liu Y,Chen F,Luo W,Bu Y,Qin J,Guo M,Qiu M,Sun L,Liu R,Wang P,Hwa J,Tang WH

12-9459-42 was used in Flow cytometry/Cell sorting to demonstrate a requirement for GSDMD in triggering platelet pyroptosis in cecal ligation and puncture (CLP)-induced sepsis.

Tue Aug 16 00:00:00 EDT 2022

Plasma Extracellular Vesicle Subtypes May be Useful as Potential Biomarkers of Immune Activation in People With HIV.

Pathogens & immunity

Bazié WW,Boucher J,Vitry J,Goyer B,Routy JP,Tremblay C,Trottier S,Jenabian MA,Provost P,Alary M,Gilbert C

Published figure using CD45 monoclonal antibody (Product # 12-9459-42) in Flow Cytometry

Sat Apr 23 00:00:00 EDT 2022

VAP-PLGA microspheres (VAP-PLGA) promote adipose-derived stem cells (ADSCs)-induced wound healing in chronic skin ulcers in mice via PI3K/Akt/HIF-1α pathway.

Bioengineered

Jiang W,Zhang J,Zhang X,Fan C,Huang J

Published figure using CD45 monoclonal antibody (Product # 12-9459-42) in Flow Cytometry

Wed Dec 01 00:00:00 EST 2021

Transitions in lineage specification and gene regulatory networks in hematopoietic stem/progenitor cells over human development.

Cell reports

Roy A,Wang G,Iskander D,O'Byrne S,Elliott N,O'Sullivan J,Buck G,Heuston EF,Wen WX,Meira AR,Hua P,Karadimitris A,Mead AJ,Bodine DM,Roberts I,Psaila B,Thongjuea S

12-9459-42 was used in Flow cytometry/Cell sorting to demonstrate the utility of this dataset for understanding aberrant hematopoiesis through comparison to two cancers that present at distinct time points in postnatal life-juvenile myelomonocytic leukemia, a childhood cancer, and myelofibrosis, which classically presents in older adults.

Tue Sep 14 00:00:00 EDT 2021

Bcl-xL mutant promotes cartilage differentiation of BMSCs by upregulating TGF-β/BMP expression levels.

Experimental and therapeutic medicine

Xiao K,Yang L,Xie W,Gao X,Huang R,Xie M

Published figure using CD45 monoclonal antibody (Product # 12-9459-42) in Flow Cytometry

Thu Jul 01 00:00:00 EDT 2021

Microglia use TAM receptors to detect and engulf amyloid β plaques.

Nature immunology

Huang Y,Happonen KE,Burrola PG,O'Connor C,Hah N,Huang L,Nimmerjahn A,Lemke G

12-9459-42 was used in Flow cytometry/Cell sorting to investigate if the TAM system is an essential mediator of microglial recognition and engulfment of amyloid plaques and if TAM-driven microglial phagocytosis does not inhibit, but rather promotes, dense-core plaque development.

Sat May 01 00:00:00 EDT 2021

Genome-wide Screens Identify Lineage- and Tumor-Specific Genes Modulating MHC-I- and MHC-II-Restricted Immunosurveillance of Human Lymphomas.

Immunity

Dersh D,Phelan JD,Gumina ME,Wang B,Arbuckle JH,Holly J,Kishton RJ,Markowitz TE,Seedhom MO,Fridlyand N,Wright GW,Huang DW,Ceribelli M,Thomas CJ,Lack JB,Restifo NP,Kristie TM,Staudt LM,Yewdell JW

12-9459-42 was used in Flow Cytometry to identify the global regulatory networks controlling antigen presentation.

Tue Jan 12 00:00:00 EST 2021

Down-Regulated Exosomal MicroRNA-221 - 3p Derived From Senescent Mesenchymal Stem Cells Impairs Heart Repair.

Frontiers in cell and developmental biology

Sun L,Zhu W,Zhao P,Zhang J,Lu Y,Zhu Y,Zhao W,Liu Y,Chen Q,Zhang F

Published figure using CD45 monoclonal antibody (Product # 12-9459-42) in Flow Cytometry

Mon Sep 28 00:00:00 EDT 2020

Single residue in CD28-costimulated CAR-T cells limits long-term persistence and antitumor durability.

The Journal of clinical investigation

Guedan S,Madar A,Casado-Medrano V,Shaw C,Wing A,Liu F,Young RM,June CH,Posey AD

Published figure using CD45 monoclonal antibody (Product # 12-9459-42) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2020

Platelets Fuel the Inflammasome Activation of Innate Immune Cells.

Cell reports

Rolfes V,Ribeiro LS,Hawwari I,Böttcher L,Rosero N,Maasewerd S,Santos MLS,Próchnicki T,Silva CMS,Wanderley CWS,Rothe M,Schmidt SV,Stunden HJ,Bertheloot D,Rivas MN,Fontes CJ,Carvalho LH,Cunha FQ,Latz E,Arditi M,Franklin BS

12-9459-42 was used in Flow Cytometry to conclude that platelets provide an additional layer of regulation of inflammasomes and IL-1-driven inflammation.

Tue May 12 00:00:00 EDT 2020

CAR T Cells Targeting MISIIR for the Treatment of Ovarian Cancer and Other Gynecologic Malignancies.

Molecular therapy : the journal of the American Society of Gene Therapy

Rodriguez-Garcia A,Sharma P,Poussin M,Boesteanu AC,Minutolo NG,Gitto SB,Omran DK,Robinson MK,Adams GP,Simpkins F,Powell DJ

12-9459 was used in Flow cytometry/Cell sorting to indicate that MISIIR targeting for the treatment of ovarian cancer and other gynecologic malignancies is achievable using CAR technology.

Wed Feb 05 00:00:00 EST 2020

TGF‑β induces periodontal ligament stem cell senescence through increase of ROS production.

Molecular medicine reports

Fan C,Ji Q,Zhang C,Xu S,Sun H,Li Z

Published figure using CD45 monoclonal antibody (Product # 12-9459-42) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2019

Microglia innately develop within cerebral organoids.

Nature communications

Ormel PR,Vieira de Sá R,van Bodegraven EJ,Karst H,Harschnitz O,Sneeboer MAM,Johansen LE,van Dijk RE,Scheefhals N,Berdenis van Berlekom A,Ribes Martínez E,Kling S,MacGillavry HD,van den Berg LH,Kahn RS,Hol EM,de Witte LD,Pasterkamp RJ

Published figure using CD45 monoclonal antibody (Product # 12-9459-42) in Flow Cytometry

Tue Oct 09 00:00:00 EDT 2018

A fully defined static suspension culture system for large-scale human embryonic stem cell production.

Cell death & disease

Li X,Ma R,Gu Q,Liang L,Wang L,Zhang Y,Wang X,Liu X,Li Z,Fang J,Wu J,Wang Y,Li W,Hu B,Wang L,Zhou Q,Hao J

Published figure using CD45 monoclonal antibody (Product # 12-9459-42) in Flow Cytometry

Thu Aug 30 00:00:00 EDT 2018

PLoS neglected tropical diseases

Narasimhan PB,Akabas L,Tariq S,Huda N,Bennuru S,Sabzevari H,Hofmeister R,Nutman TB,Tolouei Semnani R

Published figure using CD45 monoclonal antibody (Product # 12-9459-42) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2018

PLoS neglected tropical diseases

Narasimhan PB,Akabas L,Tariq S,Huda N,Bennuru S,Sabzevari H,Hofmeister R,Nutman TB,Tolouei Semnani R

Published figure using CD45 monoclonal antibody (Product # 12-9459-42) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2018

EDAG promotes the expansion and survival of human CD34+ cells.

PloS one

Zhao K,Zheng WW,Dong XM,Yin RH,Gao R,Li X,Liu JF,Zhan YQ,Yu M,Chen H,Ge CH,Ning HM,Yang XM,Li CY

12-9459 was used in Flow cytometry/Cell sorting to provide novel insight into EDAG in regulation of expansion and survival of human hematopoietic stem/progenitor cells.

Tue Feb 06 00:00:00 EST 2018

Renal Sodium Gradient Orchestrates a Dynamic Antibacterial Defense Zone.

Cell

Berry MR,Mathews RJ,Ferdinand JR,Jing C,Loudon KW,Wlodek E,Dennison TW,Kuper C,Neuhofer W,Clatworthy MR

12-9459-42 was used in Flow Cytometry to reveal a critical accessory role for the homeostatic function of a vital organ in optimizing tissue defense.

Thu Aug 24 00:00:00 EDT 2017

Nrf2 Inhibits Periodontal Ligament Stem Cell Apoptosis under Excessive Oxidative Stress.

International journal of molecular sciences

Liu Y,Yang H,Wen Y,Li B,Zhao Y,Xing J,Zhang M,Chen Y

12-9459 was used in Flow cytometry/Cell sorting to analyze novel mechanisms underlying Nrf2-mediated anti-apoptosis in periodontal ligament stem cells in the periodontitis oxidative microenvironment.

Wed May 17 00:00:00 EDT 2017

Increased expression of triggering receptor expressed on myeloid cells-1 in the population with obesity and insulin resistance.

Obesity (Silver Spring, Md.)

Subramanian S,Pallati PK,Rai V,Sharma P,Agrawal DK,Nandipati KC

Published figure using CD45 monoclonal antibody (Product # 12-9459-42) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2017

Etanercept-Synthesising Mesenchymal Stem Cells Efficiently Ameliorate Collagen-Induced Arthritis.

Scientific reports

Park N,Rim YA,Jung H,Kim J,Yi H,Kim Y,Jang Y,Jung SM,Lee J,Kwok SK,Park SH,Ju JH

12-9459 was used in Flow cytometry/Cell sorting to develop a novel method to produce synthetic biological drugs from engineered MSCs, and investigate the anti-arthritic effect of these cells in a collagen-induced arthritis mouse model.

Fri Jan 13 00:00:00 EST 2017

Tumor immune microenvironment characterization in clear cell renal cell carcinoma identifies prognostic and immunotherapeutically relevant messenger RNA signatures.

Genome biology

Şenbabaoğlu Y,Gejman RS,Winer AG,Liu M,Van Allen EM,de Velasco G,Miao D,Ostrovnaya I,Drill E,Luna A,Weinhold N,Lee W,Manley BJ,Khalil DN,Kaffenberger SD,Chen Y,Danilova L,Voss MH,Coleman JA,Russo P,Reuter VE,Chan TA,Cheng EH,Scheinberg DA,Li MO,Choueiri TK,Hsieh JJ,Sander C,Hakimi AA

Published figure using CD45 monoclonal antibody (Product # 12-9459-42) in Flow Cytometry

Thu Nov 17 00:00:00 EST 2016

A Member of the Nuclear Receptor Superfamily, Designated as NR2F2, Supports the Self-Renewal Capacity and Pluripotency of Human Bone Marrow-Derived Mesenchymal Stem Cells.

Stem cells international

Zhu N,Wang H,Wang B,Wei J,Shan W,Feng J,Huang H

12-9459 was used in Flow cytometry/Cell sorting to determine NR2F2 plays an important role in the stemness maintenance of bone marrow-derived mesenchymal stem cells.

Tue Jan 19 00:00:00 EST 2016

Human adipose stem cell and ASC-derived cardiac progenitor cellular therapy improves outcomes in a murine model of myocardial infarction.

Stem cells and cloning : advances and applications

Davy PM,Lye KD,Mathews J,Owens JB,Chow AY,Wong L,Moisyadi S,Allsopp RC

12-9459 was used in Flow cytometry/Cell sorting to investigate the potential use of adipose-derived stem cells.

Wed Nov 25 00:00:00 EST 2015

A novel autosomal recessive TERT T1129P mutation in a dyskeratosis congenita family leads to cellular senescence and loss of CD34+ hematopoietic stem cells not reversible by mTOR-inhibition.

Aging

Stockklausner C,Raffel S,Klermund J,Bandapalli OR,Beier F,Brümmendorf TH,Bürger F,Sauer SW,Hoffmann GF,Lorenz H,Tagliaferri L,Nowak D,Hofmann WK,Buergermeister R,Kerber C,Rausch T,Korbel JO,Luke B,Trumpp A,Kulozik AE

12-9459 was used in Flow cytometry/Cell sorting to outline limitations of a potential dyskeratosis congenita therapy based on rapamycin.

Sun Nov 01 00:00:00 EDT 2015

Vascular niche promotes hematopoietic multipotent progenitor formation from pluripotent stem cells.

The Journal of clinical investigation

Gori JL,Butler JM,Chan YY,Chandrasekaran D,Poulos MG,Ginsberg M,Nolan DJ,Elemento O,Wood BL,Adair JE,Rafii S,Kiem HP

Published figure using CD45 monoclonal antibody (Product # 12-9459-42) in Flow Cytometry

Mon Mar 02 00:00:00 EST 2015

Mesenchymal stromal cells form vascular tubes when placed in fibrin sealant and accelerate wound healing in vivo.

Biomaterials

Mendez JJ,Ghaedi M,Sivarapatna A,Dimitrievska S,Shao Z,Osuji CO,Steinbacher DM,Leffell DJ,Niklason LE

12-9459 was used in Flow cytometry/Cell sorting to show that ADSCs are able to form tubular structures within fibrin gels, and may also contribute to faster wound healing.

Sun Feb 01 00:00:00 EST 2015

Epithelial cell differentiation of human mesenchymal stromal cells in decellularized lung scaffolds.

Tissue engineering. Part A

Mendez JJ,Ghaedi M,Steinbacher D,Niklason LE

Published figure using CD45 monoclonal antibody (Product # 12-9459-42) in Flow Cytometry

Sun Jun 01 00:00:00 EDT 2014

Aberrant expression of SALL4 in acute B cell lymphoblastic leukemia: mechanism, function, and implication for a potential novel therapeutic target.

Experimental hematology

Ueno S,Lu J,He J,Li A,Zhang X,Ritz J,Silberstein LE,Chai L

12-9459 was used in Flow cytometry/Cell sorting to show that SALL4 plays a key role in B-ALL cell survival and could be a potential novel target in B cell lymphoblastic leukemia treatment.

Tue Apr 01 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: CD45; CD45 antigen; L-CA; Leukocyte common antigen; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen; T220 and B220

Gene Aliases: B220; CD45; CD45R; GP180; L-CA; LCA; LY5; PTPRC; T200

UniProt ID: (Human) P08575

Entrez Gene ID: (Human) 5788

Molecular Function: protein phosphatase protein modifying enzyme