Staining of C57Bl/6 splenocytes with Anti-Mouse CD3e APC (Product # 17-0031-82) and 0.125 µg of Anti-Mouse V beta 10b TCR Biotin (right) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.

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Product Details

13-5805-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

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Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, lambda

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

B21.5

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

100 µg

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4°C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

AB_10670366

Product Specific Information

Description: The B21.5 monoclonal antibody reacts with the Vbeta10 T Cell Receptor (TCR) in mice of the b haplotype of the TCR Vbeta gene complex, which includes AKR, BALB/c, CBA, C3H/He, C57BL, DBA/1 and DBA/2 mice. It does not react with Vbeta10 in mice of the a or c haplotypes of the TCR Vbeta gene complex, including C57BR, C57L, SJL, and SWR mice.

Applications Reported: This B21.5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This B21.5 antibody has been tested by flow cytometric analysis of C57Bl/6 mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The ability of T cell receptors (TCR) to discriminate foreign from self-peptides presented by major histocompatibility complex (MHC) class II molecules is essential for an effective adaptive immune response. TCR recognition of self-peptides has been linked to autoimmune disease. Mutant self-peptides have been associated with tumors. Engagement of TCRs by a family of bacterial toxins know as superantigens has been responsible for toxic shock syndrome. Autoantibodies to V beta segments of T cell receptors have been isolated from patients with rheumatoid arthritis (RA) and systemic lupus erythematosus (SLE). The autoantibodies block TH1-mediated inflammatory autodestructive reactions and are believed to be a method by which the immune system compensates for disease (ref5). T Cell and TCR Diversity Most human T cells express the TCR alpha-beta and either CD4 or CD8 molecule (single positive, SP). A small number of T cells lack both CD4 and CD8 (double negative, DN). Increased percentages of alpha-beta DN T cells have been identified in some autoimmune and immunodeficiency disorders. Gamma-delta T cells are primarily found within the epithelium. They show less TCR diversity and recognize antigens differently than alpha-beta T cells. Subsets of gamma-delta T cells have shown antitumor and immunoregulatory activity.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Bioinformatics

Gene Aliases: TCRbeta; Tib

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Entrez Gene ID: (Mouse) 21577