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Invitrogen
CD262 (DR5) Monoclonal Antibody (MD5-1), Biotin, eBioscience™
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-Flow-20170216130641.jpg?time=20220826 "CD262 (DR5) Antibody in Flow Cytometry (Flow)")
FIGURE: 1 / 1
CD262 (DR5) Antibody (13-5883-82) in Flow
Staining of non-transfected (left) and mouse DR5 transfected (right) BHK cells with 0.06 µg of Golden Syrian Hamster IgG Isotype Control Biotin (Product # 13-4914-85) (open histogram) or 0.06 µg of Anti-Mouse CD262 (DR5) Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87). Total viable cells were used for analysis.
-Flow-20170216130641.jpg?time=20220826)
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
-Flow-20170216130641.jpg?time=20220826?time=20220826 "CD262 (DR5) Antibody (13-5883-82) in Flow")
Product Details
13-5883-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Mouse
Host/Isotype
Armenian hamster
/ IgG
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
MD5-1
Conjugate
Biotin
Biotin
Biotin
Form
Liquid
Concentration
0.5 mg/mL
Amount
100 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4°C, do not freeze
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Description: The MD5-1 monoclonal antibody reacts with mouse DR5, also known as TRAIL-R2, Apo2, TRICK2, and KILLER. DR5 binds to TRAIL, activates NF-kappaB, and induces TRAIL-mediated apoptosis. DR5 mRNA is expressed broadly by normal tissue and human antigen is also expressed by some tumor cell lines.
Applications Reported: The MD5-1 antibody has been reported for use in flow cytometric analysis.
Applications Tested: The MD5-1 antibody has been tested by flow cytometric analysis of mouse DR5-transfected cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Filtration: 0.2 µm post-manufacturing filtered.
Target Information
DR5 (Apo2, TRAIL-R2, TRICK2, KILLER) is a recently identified death domain containing receptor for TRAIL, which mediates TRAIL induced apoptosis. DR5 is a member of the TNF-receptor superfamily, and contains an intracellular death domain. DR5 can be activated by tumor necrosis factor-related apoptosis inducing ligand (TNFSF10/TRAIL/APO-2L), and transduces an apoptosis signal. Studies with FADD-deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by DR5. Two transcript variants encoding different isoforms of DR5 and one non-coding transcript have been found. Overexpression of DR5 induces apoptosis and activates NF-kappa-B. DR5 is expressed in a number of cell types, and to particularly high levels in lymphocytes and spleen. Diseases associated with DR5 dysfunction include squamous cell carcinoma, and diffuse infiltrative lymphocytosis syndrome.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: death receptor 5; dr-5; KILLER/DR5 TRAIL death-inducing receptor; sCD262; soluble CD262; unnamed protein product
Gene Aliases: DR5; KILLER; Ly98; MK; TRAILR2; TRICK2A; TRICK2B; TRICKB
UniProt ID: (Mouse) Q9QZM4
Entrez Gene ID: (Mouse) 21933
apoptotic process
signal transduction
extrinsic apoptotic signaling pathway via death domain receptors
response to endoplasmic reticulum stress
TRAIL-activated apoptotic signaling pathway
regulation of apoptotic process
positive regulation of apoptotic process
negative regulation of multicellular organismal process
intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress
Disclaimer
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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